Elsevier

Brain Research

Volume 479, Issue 1, 6 February 1989, Pages 162-166
Brain Research

Short communication
Steady-state levels of pro-dynorphin-related end-products from the brain of the amphibian, Xenopus laevis

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Abstract

Steady-state analyses of prodynorphin-derived opioid peptides were conducted on acid extracts of the brain of the frog, Xenopus laevis. Radioimmunoassays specific for dynorphin A(1–17), dynorphin A(1–8), α-neoendorphin and dynorphin B coupled with gel filtration chromatography and reverse phase high performance liquid chromatography were used. The major prodynorphin-related end-product detected was α-neoendorphin. Leu-enkephalin was also detected. Since the Xenopus proenkephalin precursor does not contain the Leu-enkephalin sequence, these data suggest that some of the prodynorphin-related end-products had been cleaved to yield Leu-enkephalin.

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This research was supported in part by NIH Grant HD23100 and BRS Grant RR07138.

1

The authors wish to thank Dr. H. Akil and Dr. S. Watson (University ofMichigan) and Dr. I. Lindberg (Louisiana State University) for their generous gifts of antisera which made this project possible. We alson thank Virginia Christensen for her help in the preparation of the figures for this manuscript.

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