Elsevier

Brain Research

Volume 367, Issues 1–2, 5 March 1986, Pages 301-308
Brain Research

Three-dimensional representation and cortical projection topography of the nucleus basalis (Ch4) in the macaque: concurrent demonstration of choline acetyltransferase and retrograde transport with a stabilized tetramethylbenzidine method for horseradish peroxidase

https://doi.org/10.1016/0006-8993(86)91607-0Get rights and content

Abstract

Ninety-six percent of the nucleus basalis neurons that project to the neocortex contain choline acetyltransferase. These projections from the cholinergic component of the nucleus basalis (Ch4) are topographically organized so that each cortical area receives most of its cholinergic input from a different Ch4 sector. The three-dimensional reconstruction of these sectors reveals the presence of a complex structure. A stabilization procedure that was used in these experiments maintains all the advantages of the tetramethylbenzidine method for horseradish peroxidase while eliminating the vulnerability of the reaction-product to high pH and dehydrating agents.

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Supported in part by the ADRDA Faculty Scholar Award (to E.J.M.), the Javits Neuroscience Investigator Award; the McKnight Foundation, an Alzheimer's Research Center NIA AG05134, NS-17661 and HD-04583.

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We want to thank Leah Christie, Terry Martin, Rick Plourde and Marcia Williams for expert secretarial, technical and artistic assistance. We also want to thank Dr. D.B. Rye who helped us with the histochemical and immunohistochemical preparations.

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