Elsevier

Brain Research

Volume 298, Issue 2, 30 April 1984, Pages 235-241
Brain Research

A sensitive low artifact TMB procedure for the demonstration of WGA-HRP in the CNS

https://doi.org/10.1016/0006-8993(84)91423-9Get rights and content

Abstract

A TMB reaction with increased sensitivity and lower artifact can be obtained by dividing the Mesulam reaction into two stages. Use of the modified reaction with HRP conjugated wheat germ agglutinin (WGA-HRP) yields a powerful technique for anterograde and retrograde tracing.

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