Biochimica et Biophysica Acta (BBA) - Bioenergetics
Regular paperMediation of glycoslated and partially-deglycosylated glucose oxidase of Aspergillus niger by a ferrocene-derivatised detergent
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Effects of N-linked glycans of bilirubin oxidase on direct electron transfer-type bioelectrocatalysis
2022, BioelectrochemistryCitation Excerpt :Conversely, enzyme deglycosylation is considered to be a good option for enhancing ET kinetics. Deglycosylation of enzymes near the electrode-active site shortens the distance between the electrode-active site buried inside the enzyme and the electrode, enhancing ET kinetics [46–49]. In addition, several studies have reported improvements in DET-type bioelectrocatalytic performance by deglycosylation with the effect of downsizing [50,51].
Engineering glucose oxidase for bioelectrochemical applications
2019, BioelectrochemistryCitation Excerpt :In 1989, Kalisz et al. [173] reported a deglycosylated GOx from P. amagasakiense oxidizing glucose at 0 V vs. Ag/ AgCl on graphite electrode. Later, Gratzel et al. [174] reported the immobilization of a partially deglycosylated GOx from A. niger in combination with a ferrocene derivatives redox mediator on a graphite electrode. They observed a 100 fold increase in the second-order rate constant compared to a non-deglycosylated GOx, indicating a better electron transfer.
Effect of deglycosylation on the mediated electrocatalytic activity of recombinantly expressed Agaricus meleagris pyranose dehydrogenase wired by osmium redox polymer
2014, Electrochimica ActaCitation Excerpt :However, when expressed in P. pastoris PDH becomes substantially more glycosylated (30%) [15], which does not promote electrochemical communication with electrodes. Thus, taking into account the beneficial effect of enzyme deglycosylation observed for both direct [17–22] and mediated electron transfer [23–25], it was demonstrated that deglycosylation of AmPDH increases significantly the current output of graphite electrodes modified with deglycosylated AmPDH and Os-RP [16]. The present study aims at providing a detailed comparison between amperometric biosensors based on recombinantly expressed glycosylated (gAmPDH) and deglycosylated PDH (dgAmPDH) “wired” with Os-RP.
Recombinant glucose oxidase from Penicillium amagasakiense for efficient bioelectrochemical applications in physiological conditions
2011, Journal of BiotechnologyCitation Excerpt :This overglycosylation can affect the catalytic properties of the enzyme (Frederick et al., 1990; Kohen et al., 1997) and can prevent its use for the construction of bioelectrochemical devices (Alvarez-Icaza et al., 1995; Courjean et al., 2009). Even though deglycosylation has only marginal effects on GOX biochemical properties, it can significantly improve the bioelectrochemical characteristics of enzyme-based electrodes (Akhtar and Bhakuni, 2003; Courjean et al., 2009; Fraser et al., 1992; Kalisz et al., 1997; Prévoteau et al., 2010). The first advantage of the recombinant yGOXpenag overexpressed in P. pastoris is that it displays similar glycosylation content (14 wt%) than the native enzyme (13 wt%) and can easily be deglycosylated without affecting its catalytic properties (Kalisz et al., 1997; Witt et al., 1998).
Breaking the barrier to fast electron transfer
2009, BioelectrochemistryTRANSITION METAL CHEMISTRY OF GLUCOSE OXIDASE, HORSERADISH PEROXIDASE, AND RELATED ENZYMES
2004, Advances in Inorganic ChemistryCitation Excerpt :This impediment has been overcome in part by showing that the electrocatalytic activity of ferrocenes toward GO stays high in the presence of neutral, positively, and negatively charged surfactants (87,88). These studies followed the report of Fraser et al. (89) on a ferrocene-derivatized detergent, 11-(ferrocenylundecyl)trimethylammonium bromide. It is an electron acceptor for GO(red) when electrochemically oxidized to the corresponding ferricenium ion.