Robust G2 pausing of adult stem cells in Hydra
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open access
Keywords
Flow cytometry method
Epithelial stem cells
Interstitial stem cells
G2 pausing
Cell cycling
AEP_cnnos1 and AEP_Icy1 transgenic Hydra
Abbreviations
ASCs
adult stem cells
BLI
BrdU-labeling index
BrdU
5-bromo-2׳-deoxyuridine
CV
coefficient of variation
DM
dissociation medium
eGFP
enhanced green fluorescent protein
ESCs
embryonic stem cells
FC
flow cytometry
FSC
forward scatter
HS
heat-shock
IPs
interstitial progenitors
ISCs
interstitial stem cells
MECs
myoepithelial cells
NDZ
nocodazole
PI
propidium iodide
SCs
stem cells
SSC
side scatter
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Copyright © 2014 International Society of Differentiation. Published by Elsevier Inc.