Crystal structure of a soluble form of the human T cell coreceptor CD8 at 2.6 Å resolution

doi:10.1016/0092-8674(92)90085-Q

Cell

Volume 68, Issue 6, 20 March 1992, Pages 1145-1162

https://doi.org/10.1016/0092-8674(92)90085-Q Get rights and content

Abstract

A secreted fragment of the extracellular portion of human CD8α has been expressed in CHO cells, and a deglycosylated and proteolyzed form of this fragment has been crystallized. We report here the crystal structure of this fragment as refined at 2.6 Å resolution. The structure was solved by molecular replacement using a superposition of ten variable domains from immunoglobulin light chains as the search model. Only the N-terminal 114 amino acids of CD8α are visible in the electron density maps. The domain formed by these residues possesses a fold typical of immunoglobulin variable domains and associates to form F_v-like homodimers.

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Cell

ArticleCrystal structure of a soluble form of the human T cell coreceptor CD8 at 2.6 Å resolution

Abstract

Nucl. Instrum. Meth.

Cell

J. Mol. Biol.

J. Mol. Biol.

Adv. Immunol.

Cell

Cell

J. Mol. Biol.

Cell

Anal. Biochem.

Cell

Meth. Enzymol.

Meth. Enzymol.

Cell

J. Mol. Biol.

J. Mol. Struct.

Adv. Protein Chem.

Cell

J. Mol. Biol.

J. Biol. Chem.

Cell

J. Biol. Chem.

J. Biol. Chem.

Cell

Cell

Cell

Cell

Three dimensional structure of antibodies

Annu. Rev. Immunol.

Three-dimensional structure of Igs

Annu. Rev. Biochem.

Structure of the human class I histocompatibility antigen, HLA-A2

Nature

Carbohydrate composition of β-core

Endocrinology

A hypothetical model of the foreign antigen binding site of class II histocompatibility molecules

Nature

Extension of molecular replacement: a new search strategy based on Patterson correlation refinement

Acta Crystallogr.

Slow-cooling protocols for crystallographic refinement by simulated annealing

Acta Crystallogr.

Novel arrangement of immunoglobulin variable domains: X-ray crystallographic analysis of the λ-chain dimer Bence-Jones protein Loc

Biochemistry

The Lyt-2 molecule recognizes residues in the class I α3 domain in allogeneic cytotoxic T cell responses

J. Exp. Med.

Article
Crystal structure of a soluble form of the human T cell coreceptor CD8 at 2.6 Å resolution