Germline autonomy of maternal-effect mutations altering the embryonic body pattern of Drosophila

doi:10.1016/0012-1606(86)90179-X

Developmental Biology

Volume 113, Issue 2, February 1986, Pages 443-448

https://doi.org/10.1016/0012-1606(86)90179-X Get rights and content

Abstract

Nine maternal-effect loci of Drosophila melanogaster were tested in germline mosaics to determine whether the wildtype gene activity is required in somatic or germline components of the maternal ovary. Mutations in these loci affect the anterior-posterior or dorso-ventral body pattern. In all nine loci (torso, trunk, exuperantia, vasa, valois, staufen, tudor, dorsal, Toll) a mutant genotype in the germ cells is sufficient to produce all aspects of the mutant embryonic phenotype, even when those germ cells are surrounded by wildtype somatic tissues.

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Citation Excerpt :
Also known as ddx4, the vasa gene encodes for a member of a family of ATP-dependent RNA helicases that belongs to the DEAD (Asp–Glu–Ala–Asp)-box protein family. The vasa mRNA transcript has been detected in the germ cells of a wide range of organisms (Schupbach and Wieschaus, 1986; Hay et al., 1988; Liang et al., 1994; Olsen et al., 1997; Castrillon et al., 2000). In teleosts, there is a large abundance of vasa that can be evaluated for the identification of germ cell lineages, including PGC, ASGs, and oogonia, in various species (Braat et al., 2004).
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^☆: This research was supported by United States Public Health Service Grant 5RO1 HD15587 to Eric Wieschaus, by Swiss National Science Foundation Grant 3.460.79 to R. Nöthiger, and by a postdoctoral fellowship from the Swiss National Science Foundation.

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Developmental Biology

Abstract

References (18)

Modifying effects of ultraviolet irradiation on the development of abnormal body patterns in centrifuged insect embryos (Smittia sp., Chironomidae, Diptera)

Dev. Biol

Maternal effect mutations that alter the spatial coordinates of the embryos of Drosophila melanogaster

Clonal analysis of the tissue specificity of recessive female-sterile mutations of Drosophila melanogaster using a dominant female-sterile mutation Fs(1)K1237

Dev. Biol

Specification of the basic body pattern in insect embryogenesis

Adv. Ins. Physiol

Autosomal mutations that interfere with sex determination in somatic cells of Drosophila have no direct effect on the germline

Dev. Biol

A clonal analysis of the roles of somatic cells and germline during oogenesis in Drosophila

Dev. Biol

The phenogenetics of a temperature sensitive, autosomal dominant, female sterile gene in Drosophila melanogaster

Dev. Biol

Information for the dorsal-ventral pattern of the Drosophila embryo is stored as maternal mRNA

Nature (London)

Genetic analysis of three dominant female sterile mutations located on the X chromosome of Drosophila melanogaster

Genetics

Cited by (195)

A biscoumarinyl hydrazone based nontoxic fluorescent dye for direct binding and imaging of actin in living cells and organism

Comparative transcriptional analysis uncovers molecular processes in early and mature somatic cyst cells of Drosophila testes

Identification and characterization of a new germline-specific marker vasa gene and its promoter in the giant freshwater prawn Macrobrachium rosenbergii

Composite morphogenesis during embryo development

Characterization of a vasa homolog in Mekong giant catfish (Pangasianodon gigas): Potential use as a germ cell marker

Seasonal composition of immature germ cells in the Yesso scallop identified by vasa-like gene (my-vlg) and protein expression, with evidence of irregular germ cell differentiation accompanied with a high mortality event

Full paperGermline autonomy of maternal-effect mutations altering the embryonic body pattern of Drosophila☆

Abstract

Dev. Biol

Dev. Biol

Adv. Ins. Physiol

Dev. Biol

Dev. Biol

Dev. Biol

Information for the dorsal-ventral pattern of the Drosophila embryo is stored as maternal mRNA

Nature (London)

Genetic analysis of three dominant female sterile mutations located on the X chromosome of Drosophila melanogaster

Genetics

Full paper
Germline autonomy of maternal-effect mutations altering the embryonic body pattern of Drosophila☆