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Dysregulation of miR-202-3p Affects Migration and Invasion of Endometrial Stromal Cells in Endometriosis via Targeting ROCK1

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A Correction to this article was published on 25 April 2022

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Abstract

Dysregulation of microRNAs in endometrial cells plays a pivotal role in the pathogenesis of endometriosis (EM). This study aims to investigate the implication of aberrant miR-202-3p expression in EM and the underlying mechanisms. We demonstrated that miR-202-3p was significantly downregulated in eutopic endometrium of EM in comparison to normal endometrial samples (P < 0.05). Primary endometrial stromal cells (ESCs) isolated from eutopic or ectopic endometrium also showed a significant decrease in miR-202-3p level compared to ESCs from normal endometrium (P < 0.01). Functional studies using MTT, wound healing assay and transwell assay indicated that overexpression of miR-202-3p greatly impaired cell viability, migration, and invasion, whereas suppression of miR-202-3p exhibited the opposite effects (P < 0.05 or P < 0.01). miR-202-3p mimics or inhibitors transfection significantly decreased or increased expression of Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), respectively, in ESCs (P < 0.01). Using dual luciferase reporter assay, we validated ROCK1 as a direct target of miR-202-3p. Moreover, negative correlations between miR-202-3p and ROCK1 mRNA/protein levels were determined in both eutopic and normal control endometrium (P < 0.01). In conclusion, these findings suggest that suppression of miR-202-3p in ESCs results in enhanced cell viability, invasion, and migration at least partially via upregulation of its target ROCK1, which eventually contributes to the development of endometriosis.

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Acknowledgments

The authors thank the support for this research from National Natural Science Foundation of China (NSFC) (No. 81100411 to M.Z.), Zhongnan Hospital of Wuhan University Science, Technology and Innovation Seed Fund (No. cxpy20160038 to M.Z.), Hubei Province health and family planning scientific research project (No. WJ2018H0007 to M.Z.), and Health Commission of Hubei Province scientific research project (No. WJ2019Q048 to M.Z.)

Funding

The present study was supported by National Natural Science Foundation of China (NSFC) (No. 81100411 to M.Z.), Zhongnan Hospital of Wuhan University Science, Technology and Innovation Seed Fund (No. cxpy20160038 to M.Z.), Hubei Province health and family planning scientific research project (No. WJ2018H0007 to M.Z.), Health Commission of Hubei Province scientific research project (No. WJ2019Q048 to M.Z.)

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Authors and Affiliations

  1. Reproductive Medicine Center, Zhongnan Hospital, Wuhan University, No.169 East Lake Road, Wuhan, 430071, Hubei, China

    Ming Zhang, Yuanzhen Zhang, Li Li, Ling Ma & Chun Zhou

  2. Hubei Clinical Research Center for Prenatal Diagnosis and Birth Health, Wuhan, 430071, Hubei Province, People’s Republic of China

    Ming Zhang & Yuanzhen Zhang

  3. Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan University, Wuhan, 430071, Hubei Province, People’s Republic of China

    Ming Zhang & Yuanzhen Zhang

  4. Department of Obstetrics & Gynecology, Zhongnan Hospital, Wuhan University, Wuhan, 430071, Hubei Province, People’s Republic of China

    Yuanzhen Zhang

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  1. Ming Zhang
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  2. Yuanzhen Zhang
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  3. Li Li
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  4. Ling Ma
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MZ performed most of the experiments and contributed to study design and manuscript drafting; YZ revised the manuscript, LL was responsible for data analysis, LM and CZ provided aid with collection and processing of samples. All authors reviewed and approved the final manuscript.

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Correspondence to Ming Zhang.

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Zhang, M., Zhang, Y., Li, L. et al. Dysregulation of miR-202-3p Affects Migration and Invasion of Endometrial Stromal Cells in Endometriosis via Targeting ROCK1. Reprod. Sci. 27, 731–742 (2020). https://doi.org/10.1007/s43032-019-00079-4

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