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LncRNA BLACAT1 Is Upregulated in Cervical Squamous Cell Carcinoma (CSCC) and Predicts Poor Survival

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Abstract

The function of oncogenic lncRNA BLACAT1 has been studied in several types of cancer, while its role in cervical squamous cell carcinoma (CSCC) is unknown. We showed that BLACAT1 was upregulated in CSCC tissue comparing to adjacent non-cancer tissue of CSCC patients. BLACAT1 expression in CSCC tissues was not affected by HPV infection. Patients with higher BLACAT1 level in CSCC tissues showed a significantly lower overall 5-year survival rate. BLACAT1 expression was inversely correlated with several tumor-suppressive miRNAs, such as miR-424 and miR-143. miR-424 and miR-143 overexpression did not significantly affect BLACAT1, while BLACAT1 overexpression caused downregulated miR-424 and miR-143. Overexpression of miR-424 and miR-143 led to inhibited migration and invasion, while BLACAT1 overexpression led to promoted migration and invasion of CSCC cells. In addition, overexpression of miR-424 and miR-143 attenuated the effects of BLACAT1 overexpression. Therefore, BLACAT1 overexpression may promote CSCC by downregulating miR-424 and miR-143.

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References

  1. Siegel RL, Miller KD, Jemal A. Cancer statistics, 2017[J]. CA Cancer J Clin. 2017;67(1):7–30.

    Article  Google Scholar 

  2. Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin. 2011;61:69–90.

    Article  Google Scholar 

  3. Wentzensen N, Schiffman M, Palmer T, et al. Triage of HPV positive women in cervical cancer screening[J]. J Clin Virol. 2016;76:S49–55.

    Article  Google Scholar 

  4. Rodriguez-Carunchio L, Soveral I, Steenbergen RDM, et al. HPV-negative carcinoma of the uterine cervix: a distinct type of cervical cancer with poor prognosis[J]. BJOG. 2015;122(1):119–27.

    Article  CAS  Google Scholar 

  5. Malagón T, Kulasingam S, Mayrand MH, Ogilvie G, Smith L, Bouchard C, et al. Age at last screening and remaining lifetime risk of cervical cancer in older, unvaccinated, HPV-negative women: a modelling study[J]. Lancet Oncol. 2018;19(12):1569–78.

    Article  Google Scholar 

  6. Machalek DA, Wark JD, Tabrizi SN, Hopper JL, Bui M, Dite GS, et al. Genetic and environmental factors in invasive cervical cancer: design and methods of a classical twin study[J]. Twin Res Hum Genet. 2017;20(1):10–8.

    Article  Google Scholar 

  7. Wang SS, Gonzalez P, Yu K, Porras C, Li Q, Safaeian M, et al. Common genetic variants and risk for HPV persistence and progression to cervical cancer[J]. PLoS One. 2010;5(1):e8667.

    Article  Google Scholar 

  8. Gutschner T, Diederichs S. The hallmarks of cancer: a long non-coding RNA point of view[J]. RNA Biol. 2012;9(6):703–19.

    Article  CAS  Google Scholar 

  9. Spizzo R, Almeida MI, Colombatti A, Calin GA. Long non-coding RNAs and cancer: a new frontier of translational research?[J]. Oncogene. 2012;31(43):4577–87.

    Article  CAS  Google Scholar 

  10. Qi P, Du X. The long non-coding RNAs, a new cancer diagnostic and therapeutic gold mine[J]. Mod Pathol. 2013;26(2):155–65.

    Article  CAS  Google Scholar 

  11. Aalijahan H, Ghorbian S. Long non-coding RNAs, and cervical cancer. Exp Mol Pathol. 2019;106:7–16.

    Article  CAS  Google Scholar 

  12. Huang FX, Chen HJ, Zheng FX, Gao ZY, Sun PF, Peng Q, et al. LncRNA BLACAT1 is involved in chemoresistance of non-small cell lung cancer cells by regulating autophagy[J]. Int J Oncol. 2018;54(1):339–47.

    PubMed  Google Scholar 

  13. Wu X, Zheng Y, Han B, Dong X. Long noncoding RNA BLACAT1 modulates ABCB1 to promote oxaliplatin resistance of gastric cancer via sponging miR-361[J]. Biomed Pharmacother. 2018;99:832–8.

    Article  CAS  Google Scholar 

  14. Shan D, Shang Y, Hu T. Long noncoding RNA BLACAT1 promotes cell proliferation and invasion in human cervical cancer. Oncol Lett. 2018;15(3):3490–5.

    PubMed  PubMed Central  Google Scholar 

  15. Wang CH, Li YH, Tian HL, Bao XX, Wang ZM. Long non-coding RNA BLACAT1 promotes cell proliferation, migration and invasion in cervical cancer through activation of Wnt/β-catenin signaling pathway[J]. Eur Rev Med Pharmacol Sci. 2018;22(10):3002–9.

    Google Scholar 

  16. Xu J, Li Y, Wang F, Wang X, Cheng B, Ye F, et al. Suppressed miR-424 expression via upregulation of target gene Chk1 contributes to the progression of cervical cancer[J]. Oncogene. 2013;32(8):976–87.

    Article  CAS  Google Scholar 

  17. Liu L, Yu X, Guo X, Tian Z, Su M, Long Y, et al. miR-143 is downregulated in cervical cancer and promotes apoptosis and inhibits tumor formation by targeting Bcl-2[J]. Mol Med Rep. 2012;5(3):753–60.

    CAS  PubMed  Google Scholar 

  18. Lau YM, Cheung TH, Yeo W, Mo F, Yu MY, Lee KM, et al. Prognostic implication of human papillomavirus types and species in cervical cancer patients undergoing primary treatment[J]. PLoS One. 2015;10(4):e0122557.

    Article  Google Scholar 

  19. Paraskevopoulou MD, Hatzigeorgiou AG. Analyzing miRNA–lncRNA interactions[M]//long non-coding RNAs. New York, NY: Humana Press; 2016. p. 271–86.

    Book  Google Scholar 

  20. Liz J, Esteller M. lncRNAs and microRNAs with a role in cancer development[J]. Biochim Biophys Acta Gene Regul Mech 2016, 1859.

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Author notes

  1. Hailing Cheng and Jun Tian contributed equally to this work.

Authors and Affiliations

  1. Department of Gynecology, Huaihe Hospital, Henan University, Kaifeng, 475000, China

    Hailing Cheng, Jun Tian, Chen Wang, Lu Ren & Ning Wang

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  1. Hailing Cheng
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  2. Jun Tian
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  3. Chen Wang
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  4. Lu Ren
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  5. Ning Wang
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Correspondence to Ning Wang.

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The authors declare that they have no competing interests.

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Supplemental file 1

Original images of cell invasion and migration (DOCX 14494 kb)

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Cheng, H., Tian, J., Wang, C. et al. LncRNA BLACAT1 Is Upregulated in Cervical Squamous Cell Carcinoma (CSCC) and Predicts Poor Survival. Reprod. Sci. 27, 585–591 (2020). https://doi.org/10.1007/s43032-019-00058-9

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  • DOI: https://doi.org/10.1007/s43032-019-00058-9

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