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Does compatibility of wheat cultivars with Azospirillum brasilense strains affect drought tolerance?

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Abstract

Due to various physiological outcomes from different symbiosis combinations of wheat cultivars (Triticum aestivum L.) with Azospirillum brasilense strains, evaluation of compatibility of wheat cultivars with bacteria strains is essential to assess the improvement of wheat tolerance to drought condition. So, under control condition (80% of field capacity), the rate of compatibility of six wheat cultivars inoculated with A. brasilense Sp7 (as standard) or Sp245 (produce more abscisic acid) strains was determined based on the growth and biochemical parameters. According to the result Roshan-Sp245, Shahpasand-Sp7, Roshan-Sp7, and Shahpasand-Sp245 as the most compatible, most incompatible, less compatible and less incompatible were determined, respectively. In another experiment, selected combinations were compared at drought condition (40% of field capacity). All physiological parameters were significantly (P ≤ 0.05) affected due to compatibility or drought conditions. In average, under drought condition, root lengths affected positively in Roshan (tolerant) and negatively in Shahpasand (sensitive) cultivars inoculated with different strains of bacteria. Although, shoots protein significantly correlated with compatibility (r = 0.94), number of root branches correlated more with drought tolerance (r = 0.98). In Roshan-Sp245 as compatible pair, enzyme activity of TAL (tyrosine ammonia lyase) in roots, number of root branches, root’s protein, shoot’s PAL (phenylalanine ammonia lyase enzyme) and TAL activities were increased. In contrast, in Shahpasand-Sp7 as incompatible pair, these parameters decreased or did not change under drought condition. Therefore, higher tolerance to drought was obtained through the compatibility of associated pairs. However, significant correlation between compatibility, drought tolerance (r = 0.77) and different parameters were observed.

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References

  1. Amooaghaie R, Mostajeran A, Emtiazi G (2004) The effect of compatible and incompatible Azospirillum brasilense strains on proton efflux of intact wheat roots. Plant Soil 243:155–160

    Article  Google Scholar 

  2. Baldani VL, Baldani JI, Döbereiner J (1983) Effects of Azospirillum inoculation on root infection and nitrogen incorporation in wheat. Can J Microbiol 29:924–929

    Article  Google Scholar 

  3. Barnawal D, Bharti N, Pandey SS, Pandey A, Chanotiya CS, Kalra A (2017) Plant growth-promoting rhizobacteria enhance wheat salt and drought stress tolerance by altering endogenous phytohormone levels and TaCTR1/TaDREB2 expression. Physiol Plant 161:502–514

    Article  CAS  Google Scholar 

  4. Baron C, Zambryski PC (1995) The plant response in pathogenesis, symbiosis, and wounding: variations of a common theme? Annu Rev Gent 29:107–129

    Article  CAS  Google Scholar 

  5. Bashan Y, De-Bashan LE (2010) How the plant growth-promoting bacterium Azospirillum promotes plant growth—a critical assessment. Adv Agro 108:77–136

    Article  CAS  Google Scholar 

  6. Beaudoin-Eagan LD, Thorpe TA (1985) Tyrosine and phenylalanine ammonia lyase activities during shoot initiation in tobacco callus cultures. Plant Physiol 78:438–441

    Article  CAS  Google Scholar 

  7. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  8. Bray E (2000) Response to abiotic stress. In: Bailey-Serres J, Weretilnyk E (eds) Biochemistry and molecular biology of plants. American Society of Plant Physiologists, Rockville

    Google Scholar 

  9. Camilios-Neto D, Bonato P, Wassem R, Tadra-Sfeir MZ, Brusamarello-Santos LC, Valdameri G, Donatti L, Faoro H, Weiss VA, Chubatsu LS (2014) Dual RNA-seq transcriptional analysis of wheat roots colonized by Azospirillum brasilense reveals up-regulation of nutrient acquisition and cell cycle genes. BMC Genom 15:378–391

    Article  Google Scholar 

  10. Cohen AC, Bottini R, Pontin M, Berli FJ, Moreno D, Boccanlandro H, Travaglia CN, Piccoli PN (2015) Azospirillum brasilense ameliorates the response of Arabidopsis thaliana to drought mainly via enhancement of ABA levels. Physiol Plant 153:79–90

    Article  CAS  Google Scholar 

  11. Dobereiner J, Day JM (1976) Association symbiosis in tropical grasses: characterization of microorganisms and dinitrogen fixing sites. In: Newton WE, Nyman CJ (eds) Proceedings of the first international sypmosium on nitrogen fixation. Washington State University Press, Pullman, pp 518–538

    Google Scholar 

  12. El Siddig MA, Baenziger S, Dweikat I, El Hussein AA (2013) Preliminary screening for water stress tolerance and genetic diversity in wheat (Triticum aestivum L.) cultivars from Sudan. J Genet Eng Biotechnol 11:87–94

    Article  Google Scholar 

  13. Estabrook EM, Sengupta-Gopalan C (1991) Differential expression of phenylalanine ammonia lyase and chalcone synthase during soybean nodule development. Plant Cell 3:299–308

    CAS  PubMed  PubMed Central  Google Scholar 

  14. Gamas P, De Billy F, Truchet G (1998) Symbiosis-specific expression of two Medicago truncatula nodulin genes, MtN1 and MtN13, encoding products homologous to plant defense proteins. Mol Plant Microbe Int 11:393–403

    Article  CAS  Google Scholar 

  15. Hartmann A, Baldani JI (2006) The Genus Azospirillum. In: Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E (eds) The prokaryotes proteobacteria: alpha and beta subclasses. Springer, New York, pp 115–140

    Chapter  Google Scholar 

  16. Jahanbakhsh S, Chilan H, Razavi K (2017) Effects of water deficit on the physiological response, total protein, and gene expression of Rab17 in wheat (Triticum aestivum). J Plant Process Funct 5:35–42

    Google Scholar 

  17. Jain DK, Patriquin DG (1984) Root hair deformation, bacterial attachment, and plant growth in wheat-Azospirillum associations. Appl Environ Microb 48:1208–1213

    Article  CAS  Google Scholar 

  18. Kaur L, Zhawar VK (2015) Phenolic parameters under exogenous ABA, water stress, salt stress in two wheat cultivars varying in drought tolerance. Indian J Plant Physiol 20:151–156

    Article  Google Scholar 

  19. Maksimov I, Veselova S, Nuzhnaya T, Sarvarova E, Khairullin R (2015) Plant growth-promoting bacteria in regulation of plant resistance to stress factors. Russ J Plant Physl 62:715–726

    Article  CAS  Google Scholar 

  20. Mandal SM, Chakraborty D, Dey S (2010) Phenolic acids act as signaling molecules in plant-microbe symbioses. Plant Signal Behav 5:359–368

    Article  CAS  Google Scholar 

  21. Michiels K, Croes CL, Vanderleyden J (1991) Two different modes of attachment of Azospirillum brasilense Sp7 to wheat roots. J Gen Microbiol 137:241–246

    Article  Google Scholar 

  22. Nematalla MM, Younis ME, El-Shihaby OA, El-Bastawisy ZM (2002) Kinetin regulation of growth and secondary metabolism in waterlogging and salinity treated Vigna sinensis and Zea mays. Acta Physiol Plant 24:19–27

    Article  CAS  Google Scholar 

  23. Perrig D, Boiero ML, Masciarelli O, Penna C, Ruiz OA, Cassan FD, Luna MV (2007) Plant-growth-promoting compounds produced by two agronomically important strains of Azospirillum brasilense and implications for inoculant formulation. Appl Microbiol Biotechnol 75:1143–1150

    Article  CAS  Google Scholar 

  24. Rampino P, Pataleo S, Gerardi C, Mita G, Perrotta C (2006) Drought stress response in wheat: physiological and molecular analysis of resistant and sensitive genotypes. Plant, Cell Environ 29:2143–2152

    Article  CAS  Google Scholar 

  25. Saubidet MI, Fatta N, Barneix AJ (2002) The effect of inoculation with Azospirillum brasilense on growth and nitrogen utilization by wheat plants. Plant Soil 245:215–222

    Article  CAS  Google Scholar 

  26. Serraj R, Sinclair T (2002) Osmolyte accumulation: can it really help increase crop yield under drought conditions? Plant, Cell Environ 25:333–341

    Article  Google Scholar 

  27. Sriskandarajah S, Kennedy IR, Yu D, Tchan YT (1993) Effects of plant growth regulators on acetylene-reducing associations between Azospirillum brasilense and wheat. Plant Soil 153:165–178

    Article  CAS  Google Scholar 

  28. Steel RGD, Torrie JH (1960) Principles and procedures of statistics (with special reference to the biological sciences). McGraw-Hill Book Company, New York

    Google Scholar 

  29. Stets M, Alqueres S, Souza E, Schmid M, Hartmann A, Cruz L (2015) Quantification of Azospirillum brasilense FP2 bacteria in wheat roots by strain-specific quantitative PCR. Appl Environ Microb 81:6700–6709

    Article  CAS  Google Scholar 

  30. Sumner M (1990) Crop responses to Azospirillum inoculation. Adv Soil Sci 12:53–123

    Article  Google Scholar 

  31. Sweet HC, Bolton WE (1979) The surface decontamination of seeds to produce axenic seedlings. Am J Bot 66:692–698

    Article  CAS  Google Scholar 

  32. Thuler D, Floh E, Handro W, Barbosa H (2003) Plant growth regulators and amino acids released by Azospirillum sp. in chemically defined media. Lett Appl Microbiol 37:174–178

    Article  CAS  Google Scholar 

  33. Vacheron J, Desbrosses G, Bouffaud ML, Touraine B, Moënne-Loccoz Y, Muller D, Legendre L, Wisniewski-Dyé F, Prigent-Combaret C (2013) Plant growth-promoting rhizobacteria and root system functioning. Front Plant Sci 4:356–362

    Article  Google Scholar 

  34. Vasse J, De Billy F, Truchet G (1993) Abortion of infection during the Rhizobium meliloti—alfalfa symbiotic interaction is accompanied by a hypersensitive reaction. Plant J 4:555–566

    Article  Google Scholar 

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Acknowledgements

Authors wish to thank Dr. Michael Rothballer from AMP Research Unit Microbe-Plant Interactions for providing the bacterial strains. Financial support by the University of Isfahan is gratefully acknowledged.

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Authors and Affiliations

  1. Plant Science Division, Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran

    I. Dehghani & A. Mostajeran

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  1. I. Dehghani
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  2. A. Mostajeran
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Correspondence to A. Mostajeran.

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Communicated by A. Goyal.

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Dehghani, I., Mostajeran, A. Does compatibility of wheat cultivars with Azospirillum brasilense strains affect drought tolerance?. CEREAL RESEARCH COMMUNICATIONS 48, 121–129 (2020). https://doi.org/10.1007/s42976-019-00001-3

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  • DOI: https://doi.org/10.1007/s42976-019-00001-3

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