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Comparative transcriptomic analysis provides insights into transcription mechanisms of Vibrio parahaemolyticus T3SS during interaction with HeLa cells

  • Food Microbiology - Research Paper
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Abstract

Vibrio parahaemolyticus is an important foodborne pathogenic bacterium that harbors the type III secretion system 1 (T3SS1) as an essential virulence factor. However, the pathogenesis and infection mechanism mediated by T3SS1 are not entirely clarified. Similar to previous studies on other T3SS-positive bacteria, the T3SS1 needle is a major extracellular component in V. parahaemolyticus. We recently showed that the needle gene-deletion mutant (ΔvscF) exhibited markedly decreased cytotoxicity and effector translocation during interaction with HeLa cells. To further elucidate the pathogenesis of T3SS1 during host cell infection, bacterial RNA was extracted from wild-type POR-1 and ΔvscF mutants under infected condition for comparative RNA sequencing analysis in HeLa cell. The results showed that 120 differentially expressed genes (DEGs) were identified in the ΔvscF-infected group. These encoded proteins of DEGs, such as VP2088, VP2089, and VP2091, were annotated as ABC transporter system, whereas VP0757, VP1123, and VP1289 may be new transcriptional regulators. In addition, the downregulation of T3SS1 had a positive influence on the expression of T3SS2. Moreover, the transcription of the basal body is unaffected by the needle, and there was a close relation among the tip, translocon, and needle, because bacterial adenylate cyclase two-hybrid system (BACTH system) assay indicated the interaction of VP1656, VP1670, VP1693, and VP1694 (VscF). This study provides insights into transcription mechanism of T3SS1 upon infecting HeLa cell, which is expected to better clarify the T3SS1 virulent mechanism.

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Acknowledgements

We thank ENAGO (www.enago.cn) for providing linguistic assistance during the preparation of this manuscript and Dr. Dejun Ji (Yangzhou University) for useful comments on checking spelling and grammar. We also thank DECODE GENOMICE Biotechnology Corporation for Transcriptome sequencing.

Funding

This study was funded by the National Natural Science Foundation of China (31871893), the National Key Research and Development Program of China (2017YFF0208600), the Jiangsu Agricultural Independent Innovation Project (SCX (18) 2011), the National “Youth Top-notch Talent” Support Program (W0270187), the Introduction of Nanjing Agricultural University Scientific Research Grants Project (804121), the Central Guidance for Local Science and Technology Development (No. YDZX20173100004528), the Science and Technology Joint Project of the Yangtze River Delta (No.17395810102), the Jiangsu Collaborative Innovation Center of Meat Production and Processing, and the Yangzhou Hanjiang Science and Technology Bureau program (2020).

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  1. MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China

    Lele Lian, Wanjun Li, Tingyue Xue, Jianluan Ren, Fang Tang, Yongjie Liu, Feng Xue & Jianjun Dai

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  1. Lele Lian
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Lele Lian: conceptualization; data curation; writing original draft. Wanjun Li, Tingyue Xue: formal analysis. Jianluan Ren, Fang Tang, Yongjie Liu: project administration. Feng Xue, Jianjun Dai: conceptualization; funding acquisition.

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Lian, L., Li, W., Xue, T. et al. Comparative transcriptomic analysis provides insights into transcription mechanisms of Vibrio parahaemolyticus T3SS during interaction with HeLa cells. Braz J Microbiol 53, 289–301 (2022). https://doi.org/10.1007/s42770-021-00627-8

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