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Virulence characteristics and antimicrobial resistance of Aeromonas veronii biovar sobria 312M, a clinical isolate

  • Bacterial Fungal and Virus Molecular Biology - Research Paper
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Abstract

Aeromonas are bacteria widely distributed in the environment, and some species are able to cause infections in humans, of which diarrhea is the most common. The objective of this study was to evaluate the presence of virulence and antimicrobial resistance associated characteristics in A. veronii biovar sobria strain 312M isolated from diarrheal stools. For this, the genome sequencing and phenotypical tests were performed. The draft genome annotation revealed several complete pathways associated with carbon metabolism and a mucin-desulfating sulfatase which may contribute to intestine colonization, and a large number of virulence-associated genes encoding structures associated with adhesion, toxins, and secretion systems. The strain exhibited swimming and swarming motility, biofilm formation, and hemolytic activity. It was resistant to ampicillin, ampicillin/sulbactam, and amoxicillin-clavulanic acid. Although a cphA gene encoding a narrow-spectrum carbapenase was identified in the strain genome, no carbapenemase activity was detected in the antimicrobial susceptibility test. When compared with other A. veronii with complete genomes, the main differences in virulence characteristics are related to lateral flagella and type III and VI secretion systems; the antimicrobial resistance spectrum also varied among strains. The results indicated that A. veronii biovar sobria 312M presents high virulence potential and resistance to limited classes of antimicrobials.

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Acknowledgments

This work was supported by the Brazilian Program of National Institutes of Science and Technology - INCT/Brazilian Research Council - CNPq/MCT and Fundação Araucária. We thank Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for scholarships.

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Authors and Affiliations

  1. Departamento de Análises Clínicas, Universidade Federal do Paraná, Av. Prefeito Lothário Meisner 635, Curitiba, Paraná, CEP 80.210-170, Brazil

    Karoline de C. Prediger, Cibelle B. Dallagassa, Bárbara Moriel, Bruno Stefanello Vizzotto, Waldemar Volanski, Dayane Alberton & Cyntia M. T. Fadel-Picheth

  2. Universidade Franciscana, Santa Maria, Rio Grande do Sul, Brazil

    Bruno Stefanello Vizzotto

  3. Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Av. Coronel Francisco H. dos Santos 100, Curitiba, Paraná, CEP 81.531-980, Brazil

    Emanuel M. Souza & Fábio O. Pedrosa

  4. Programa de Pós-Graduação em Bioinformática, Universidade Federal do Paraná, Rua Alcides Vieira Arcoverde 1225, Curitiba, Paraná, CEP 81.520-260, Brazil

    Vinícius Weiss & Dieval Guizelini

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  1. Karoline de C. Prediger
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  2. Cibelle B. Dallagassa
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Correspondence to Cyntia M. T. Fadel-Picheth.

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Prediger, K.d.C., Dallagassa, C.B., Moriel, B. et al. Virulence characteristics and antimicrobial resistance of Aeromonas veronii biovar sobria 312M, a clinical isolate. Braz J Microbiol 51, 511–518 (2020). https://doi.org/10.1007/s42770-019-00180-5

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