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Comparative toxic effects of Eucalyptus globulus L. (Myrtales: Myrtaceae) and its green synthesized zinc oxide nanoparticles (ZnONPs) against Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae)

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Abstract

Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) causes quantitative and qualitative losses to stored cereals. The excessive use of synthetic residual insecticides led to the development of resistance. These pesticides have adverse effect on the environment. Green synthesized nanoparticles can be a good replacement of synthetic insecticides. Nanoparticles of ZnO prepared using the extract of Eucalyptus globulus L. (Myrtale: Myrtaceae) were never evaluated against R. dominica. Therefore, the present study was planned to evaluate the insecticidal potential of leaf extract of E. globulus and green synthesized zinc oxide nanoparticles (ZnONPs) against adults of R. dominica. Green synthesized ZnONPs were prepared using leaves extract of E. globulus as a simple, non-toxic and ecofriendly green material. Characterization of nanoparticles was carried out using UV- visible spectroscopy, Particle Size Analysis (PSA) and Scanning Electron Microscope (SEM) analysis. Insecticide bioassays were conducted using six concentrations (300, 600, 900, 1200, 1500, and 1800 ppm) of E. globulus extract. Similarly six dose rates (100, 200, 300, 400, 500 and 600 ppm) of green synthesized ZnONPs were evaluated after four exposure periods (3, 7, 11 and 15 days). The insect mortality due to E. globulus leaf extract was 62.5% against 1800 ppm dose rate while 80.5% mortality of insect was observed against 600 ppm dose rate of ZnONPs after 15 days exposure period. The LC50 for leaf extract of E. globulus and green synthesized ZnONPs were 1043.06 and 202.11 ppm respectively. Post treatment growth inhibition of R. dominica was 75.7% and 87.0% against extract and ZnONPs of E. globulus after 30 days. These results indicate that E. globulus leaf extract and green synthesized ZnONPs are effective against R. dominica and can be used as an eco-friendly approach for stored product pest management.

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The data sets used and/or analyzed during the current study are available from the corresponding author on request.

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Authors and Affiliations

  1. Department of Entomology, University of Agriculture, Faisalabad, Pakistan

    Muhammad Amir Siddique, Mansoor ul Hasan & Muhammad Sagheer

  2. Department of Plant Pathology, University of Agriculture, Faisalabad, Pakistan

    Shahbaz Talib Sahi

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  1. Muhammad Amir Siddique
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  2. Mansoor ul Hasan
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  3. Muhammad Sagheer
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  4. Shahbaz Talib Sahi
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Contributions

MAS and MH designed and conducted the experiment and collected the data. MAS, MH, and MS analyzed the data. MAS and MH wrote the manuscript. MAS and STS helped in apprehending the idea of this research, designing the layout of experiment and improving the write-up, format and language of this manuscript. MH and MS reviewed the manuscript, add and improved result section, edited the format of the tables according to the format of the journal. This final manuscript was ultimately perused, scrutinized and approved for final submission by all authors.

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Correspondence to Muhammad Amir Siddique.

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Siddique, M.A., Hasan, M.u., Sagheer, M. et al. Comparative toxic effects of Eucalyptus globulus L. (Myrtales: Myrtaceae) and its green synthesized zinc oxide nanoparticles (ZnONPs) against Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae). Int J Trop Insect Sci 42, 1697–1706 (2022). https://doi.org/10.1007/s42690-021-00691-5

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