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Biofabrication of aligned structures that guide cell orientation and applications in tissue engineering

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Abstract

The organized alignment of cells in various tissues plays a significant role in the maintenance of specific functions. To induce such an alignment, ideal scaffolds should simulate the characteristics and morphologies of natural tissues. Aligned structures that guide cell orientation are used to facilitate tissue regeneration and repair. We here review how various aligned structures are fabricated, including aligned electrospun nanofibers, aligned porous or channeled structures, micropatterns and combinations thereof, and their application in nerve, skeletal muscle, tendon, and tubular dentin regeneration. The future use of aligned structures in tissue engineering is also discussed.

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Acknowledgements

This work was financially supported by the National Key Research and Development Program of China (2018YFA0703000), the National Natural Science Foundation of China (81670972, 31872752), Key Research and Development Program of Zhejiang, China (2017C01054, 2018C03062, 2017C01063), and Postdoctoral Science Foundation of China (2020TQ0257, 2020M681896).

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  1. Kejie Lu and Ying Qian have contributed equally to this work.

Authors and Affiliations

  1. The Affiliated Hospital of Stomatology, School of Stomatology, Zhejiang University School of Medicine, and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou, 310006, China

    Kejie Lu, Ying Qian, Jiaxing Gong, Ziyu Zhu, Mengfei Yu & Huiming Wang

  2. The State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, Hangzhou, 310028, China

    Jun Yin & Liang Ma

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  1. Kejie Lu
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KL, YQ, MY and HW designed the research and drafted the manuscript. JG and ZZ helped organize the manuscript. KL, JG, YQ, ZZ, JY, LM, MY and HW revised and finalized the paper. All authors have read and approved the final manuscript and, therefore, have full access to all the data in the study and take responsibility for the integrity and security of the data.

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Lu, K., Qian, Y., Gong, J. et al. Biofabrication of aligned structures that guide cell orientation and applications in tissue engineering. Bio-des. Manuf. 4, 258–277 (2021). https://doi.org/10.1007/s42242-020-00104-5

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