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Role of lipopolysaccaride extracted from Alcaligenes faecalis as elicitor for the induction of plant defense against fusarium wilt

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Abstract

In the present investigation, emphasis was placed on the efficacy of lipopolysaccharide (LPS) extracted from the plant beneficial rhizobacterium Alcaligenes faecalis MSS8 for the induction of disease resistance by enhancing immune response in plants against phytopathogen Fusarium oxysporum f. sp. lycopersici (FOL). Characterization and proficiency of the rhizobacterial LPS for the induced defense response in plant against FOL was revealed by the gel activity staining of Peroxidase (PO) and Polyphenol oxidase (PPO) isoforms as compared to the untreated and only FOL treated plants. It has been observed that after 48 h of FOL challenge inoculation, in the LPS pretreated plant there was significant induction of PO and PPO isoforms. Furthermore, RT-PCR analysis for the study of relative gene expression of pathogenesis related (PR) proteins confirms the result of gel activity staining. It has been observed that expression level of LeChi9, LeGluB, LePR1a was significantly upregulated in LPS treated plants 24 h after FOL inoculation and maximum level was observed at 48 h as compared to the control and FOL treated plants. This clearly indicates the role of LPS extracted from A. faecalis as a potential biocontrol agent against fusarium wilt.

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Acknowledgements

We are thankful to Department of Microbiology and Biotechnology, Gujarat University to encourage and help us with the required facility and work and GUJCOST MRP for financial support. Special thanks to Prof. Vrinda S. Thaker and her research students, UGC-CAS Department of Biosciences, Saurashtra University, Rajkot for her support and guidance during the RT- PCR analysis of this study.

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Authors and Affiliations

  1. Department of Microbiology, University School of Sciences, Gujarat University, Ahmedabad, Gujarat, 380 009, India

    Stuti Patel & Meenu Saraf

  2. UGC-CAS Department of Biosciences, Saurashtra University, Rajkot, 360 005, India

    Vrinda Thaker & Ranjan Kuvad

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  1. Stuti Patel
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  2. Vrinda Thaker
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  3. Ranjan Kuvad
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  4. Meenu Saraf
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Correspondence to Meenu Saraf.

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Patel, S., Vrinda Thaker, Kuvad, R. et al. Role of lipopolysaccaride extracted from Alcaligenes faecalis as elicitor for the induction of plant defense against fusarium wilt. J Plant Pathol 102, 351–357 (2020). https://doi.org/10.1007/s42161-019-00425-0

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