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Immobilization of penicillin G acylase on a novel paramagnetic composite carrier with epoxy groups

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Abstract

In this work, Fe3O4 particles were first prepared by a reversed microemulsion method, and then the prepared nanoparticles were modified with 3-(trimethoxysilyl) propyl methacrylate (KH-570), a silane coupling agent, to enhance the dispersibility and biocompatibility of Fe3O4 particles. To get the graft ratio quantitatively, and further achieve the maximum loading capacity of KH-570, the characterization method for the graft ratio (Gr) was systemically explored first; reformative silicon molybdenum chromogenic (RSMC) was designed and presented a good result. In addition, the influence of operation conditions on the graft ratio was investigated and optimized. Results showed the graft ratio of KH-570 on magnetic Fe3O4 nanoparticles arrived at 178.51% under optimal conditions. In addition, it displayed KH-570 grafted onto the surface of Fe3O4 nanoparticles successfully, and only one silicon hydroxyl was bonded on the surface of Fe3O4 nanoparticles under optimal modifications. Finally, a magnetic composite carrier was prepared by grafting glycidyl methacrylate (GMA) onto the surface of the modified nanoparticles. Further, the composite was used to immobilize penicillin G acylase (PGA); in addition, the effect of GMA dosage on enzyme activity (EA), enzyme activity recovery rate (EAR), and enzyme loading rate (ELR) was studied. It was found that when the amount of GMA was 9.0%, the EA, EAR, and ELR were 9208 U/g, 88 mg/g, and 64.08%, respectively. In addition, the immobilized PGA still possess superparamagnetism, which can be well recycled and reused.

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Funding

This work was supported by National Natural Science Foundation of China (Grant No. 51563015).

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Authors and Affiliations

  1. State Key Laboratory of Gansu Advanced Non-ferrous Metal Materials, Lanzhou University of Technology, Lanzhou, 730050, China

    Xiayun Zhang, Zhenbin Chen, Ke Li, Dengmin Xie, Wenming Zhou & Ting Wang

  2. School of Materials Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, China

    Xiayun Zhang, Zhenbin Chen, Ke Li, Dengmin Xie, Wenming Zhou & Ting Wang

  3. School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, China

    Xiayun Zhang, Zhongduo Yang & Zhizhong Li

  4. School of Chemistry and Materials Science, Ludong University, Yantai, 264025, China

    Songmei Ma

  5. Department of Physics and Engineering, Frostburg State University, Frostburg, MD, 21532, USA

    Robert Burns & Zhen Liu

  6. Soft Matter and Molecular Biophysics Group, Department of Applied Physics, University of Santiago de Compostela, 15782, Santiago de Compostela, USA

    Juan M. Ruso

  7. Guangzhou Key Laboratory for Surface Chemistry of Energy Materials, New Energy Research Institute, School of Environment and Energy,, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, 5100067, China

    Zhenghua Tang

  8. Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, Guangdong Provincial Engineering and Technology Research Center for Environmental Risk Prevention and Emergency Disposal, School of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, 5100067, China

    Zhenghua Tang

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  1. Xiayun Zhang
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Zhang, X., Chen, Z., Li, K. et al. Immobilization of penicillin G acylase on a novel paramagnetic composite carrier with epoxy groups. Adv Compos Hybrid Mater 2, 720–734 (2019). https://doi.org/10.1007/s42114-019-00099-3

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