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Electrochemiluminescence Analysis of Hydrogen Peroxide Using L012 Modified Electrodes

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Abstract

In this paper, a luminol analog, L012, with a high-electrochemiluminescence (ECL) illumination is covalently assembled at indium tin oxide (ITO) surface that exhibits the ECL response to hydrogen peroxide in the solution. The ITO slide is firstly functioned with amine group that react with glutaraldehyde to introduce aldehyde group at the surface. Upon the exposure to L012 with amine group, the reaction between the aldehyde group and the amine group results in the linkage of L012 at the electrode surface. In the presence of hydrogen peroxide, enhanced ECL from L012 at the electrode surface is observed that is linearly related with the concentration of hydrogen peroxide. The detection limit is determined to be 4.3 μM (S/N = 3). The successful establishment of ECL response to hydrogen peroxide using L012 modified electrode will provide a new functioned ECL surface for the future ECL imaging of hydrogen peroxide release from single cells.

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Authors and Affiliations

  1. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China

    Yuling Wang, Dechen Jiang & Hong-Yuan Chen

Authors
  1. Yuling Wang
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  2. Dechen Jiang
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  3. Hong-Yuan Chen
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Correspondence to Dechen Jiang.

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Wang, Y., Jiang, D. & Chen, HY. Electrochemiluminescence Analysis of Hydrogen Peroxide Using L012 Modified Electrodes. J. Anal. Test. 4, 122–127 (2020). https://doi.org/10.1007/s41664-020-00134-z

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  • DOI: https://doi.org/10.1007/s41664-020-00134-z

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