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Chemical composition and antifungal activity of five essential oils and their major components against Fusarium oxysporum f. sp. albedinis of Moroccan palm tree

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Abstract

The effects of essential oils (EOs) and their eight major components extracted from five aromatic and medicinal plant (AMP) species on the development of Fusarium oxysporum f. sp. albedinis, the phytopathogenic fungus responsible for fusarium wilt (referred to as Bayoud in Morocco) on date palm in Moroccan oases (Errachidia region), were studied. The EOs were extracted from the plants by hydrodistillation and chemically analyzed by gas chromatography–mass spectrometry. The major chemical components extracted from the EOs of each AMP were: Origanum compactum (carvacrol 29.26%, thymol 18.52%, O-cymene 11.89%, γ-terpinene 10.77%); Thymus satureioides (borneol 22.73%, carvacrol 16.96%, α-terpineol: 12.20%); Rosmarinus officinalis (eucalyptol 26.45%, camphor 15.51%, α- and β-pinene 21.31%); Lavandula dentata (linalool 30.22%, linalyl anthranilate 23.47%, eucalyptol 9.17%); Myrtus communis (myrtenyl acetate 50.28%, eucalyptol 15.01%). The antifungal activity of the isolated EOs and their major components in comparison to that of two synthetic fungicides was evaluated by calculating the minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) of each antifungal product. The results of this analysis showed that the EOs of O. compactum and T. satureioides were able to inhibit the growth of F. oxysporum at very low concentrations (MFC 5 and 13 μl ml−1, respectively); these values were relatively better than those of the mancozeb synthetic fungicide (MFC 6 μl ml−1) and very much better than the EOs of R. officinalis, L. dentata and M. communis (MFC 40 μl ml−1). Thymol and carvacrol were effective fungicides at a very low concentration (MFC 0.94 and 2.08 μl ml−1, respectively), followed in increasing concentration by α-terpineol (MFC 10 μl ml−1), linalool (MFC 13.00 μl ml−1) and borneol (MFC 26.67 μl ml−1). α-Pinene, eucalyptol and myrtenyl acetate were shown to have comparable fungicide properties, but at high concentrations ranging from 20 to 80 μl ml−1. The effectiveness of the EO of Origanum and Thymus species would therefore appear to be linked to the abundant presence of carvracrol and thymol compounds. Given the damage caused by F. oxysporum on date palm trees and other plants in Morocco, as well as the environmental and health impacts of fungicides, the use of O. compactum and T. satureioides EOs or their major components (thymol and carvacrol) against this pathogen could be an alternative to commercially available synthetic fungicides.

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References

  • Alam MS, Kaur G, Jabbar Z, Javed K, Athar M (2007) Eruca sativa seeds possess antioxidant activity and exert a protective effect on mercuric chloride induced renal toxicity. Food Chem Toxicol 45(6):910–920

    Article  Google Scholar 

  • Badawy EIM, Samir A (2014) Composition and antimicrobial activity of essential oils isolated from Egyptian plants against plant pathogenic bacteria and fungi. Ind Crops Prod 52:776–782

    Article  Google Scholar 

  • Bakhy K, Benlhabib O, Bighelli A, Casanova J, Tomi F, Al Faiz Ch (2014) Yield and chemical variability of the essential oil isolated from aerial parts of wild Origanum compactum Benth. from Moroccan Western Rif. Am J Essent Oils Nat Prod 1(4):9–17

    Google Scholar 

  • Ben Hsouna A, Hamdi N, Miladi R, Abdelkafi S (2014) Myrtus communis essential oil: chemical composition and antimicrobial activities against food spoilage pathogens. Chem Biodivers. 11(4):571–580

    Article  Google Scholar 

  • Chifiriuc C, Grumezescu V, Grumezescu AM, Saviuc C, Lazăr V, Andronescu E (2012) Hybrid magnetite nanoparticles/Rosmarinus officinalis essential oil nanobiosystem with antibiofilm activity. Nanoscale Res Lett 7:209

    Article  Google Scholar 

  • Chryssavgi G, Vassiliki P, Athanasios M, Kibouris T, Komaitis M (2008) Essential oil composition of Pistacia lentiscus L. and Myrtus communis L.: evaluation of antioxidant capacity of methanolic extracts. Food Chem 107:1120–1130

    Article  Google Scholar 

  • Clinical and Laboratory Standards Institute (CLSI) (2008). Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; approved standard-second edition. CLSI document M38-A2. CLSI, Wayne

  • Dafereraa DJ, Ziogasb BN, Polissiou MG (2003) The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusarium sp. and Clavibacter michiganensis sp. michiganensis. Crop Prot 22:39–44

    Article  Google Scholar 

  • Djerbi M (1988) Les maladies du palmier dattier. Projet régional de lutte contre le Bayoud. FAO, Alger

    Google Scholar 

  • Djerbi M, Sedra MH, Idrissi Ammari MA (1985) Caractéristiques culturales et identification du Fusarium oxysporum f.sp. albedinis, agent causal du Bayoud. Annales de l’Institut National de la Recherche Agronomique de Tunisie 58(1):1–8

    Google Scholar 

  • Dob T, Dahmane D, Tayeb B, Chelghoum C (2015) Chemical composition of the essential oil of Lavandula dentata L. from Algeria. Int J Aromather 15:110–114

    Article  Google Scholar 

  • El Bouzidi L, Chaima AJ, Bekkouche K, Hassani L, Wohlmuth H, Leach D, Abbad A (2013) Chemical composition, antioxidant and antimicrobial activities of essential oils obtained from wild and cultivated Moroccan Thymus species. Ind Crops Prod 43:450–456

  • Essarioui A, Sedra MH (2017) Lutte contre la maladie du bayoud par solarisation et fumigation du sol. Une expérimentation dans les palmeraies du Maroc. Cah Agric 26(4):45010

    Article  Google Scholar 

  • Fennane M, Ibn Tattou M, Mathez J, Ouyahia A, El Oualidi J (1999) Flore pratique du Maroc: manuel de détermination des plantes vasculaires 1 Pteridophyta, Gymnospermae, Angiospermae (Lauraceae—Neuradaceae). Travaux de l’Institut Scientifique de Rabat, Série. Botanique no. 36, vol. 1. Morocco

  • Fernandez D, Lourd M, Ouinten M, Tantaoui A, Geige JP (1995) Le Bayoud du palmier dattier, une maladie qui menace la phœniciculture. Phytoma La Défense des Végétaux 469:36–40

    Google Scholar 

  • Fraternale D, Giamperi L, Ricci D (2003) Chemical composition and antifungal activity of essential oil obtained from in vitro plants of Thymus mastichina L. J Essent Oil Res 15(4):278–281

    Article  Google Scholar 

  • Ghanmi M, Satrani MF, Thevenon K, Elyounssi M (2015) Essential oils from Origanum compactum as an alternative active ingredient against wood decay fungi. Nat Prod Indian J 11(4):135–142

    Google Scholar 

  • Imelouane B, Elbachiri A, Ankit M, Benzeid H, Khedid K (2009) Physico-chemical compositions and antimicrobial activity of essential oil of Eastern Moroccan Lavandula dentata. Int J Agric Biol 11(2):113–118

    Google Scholar 

  • Magan N, Olsen M (2004) Mycotoxins in food. Detection and control. Woodhead Publishing/Elsevier, Cambridge

    Book  Google Scholar 

  • Manganyi MC, Regnier T, Olivier EI (2015) Antimicrobial activities of selected essential oils against Fusarium oxysporum isolates and their biofilms. South Afr J Bot 99:115–121

    Article  Google Scholar 

  • Mothana RA, Alsaid MS, Hasoon SS, Al-Mosaiyb NM, Al-Rehaily AJ, Al-Yahya MA (2012) Antimicrobial and antioxidant activities and gas chromatography mass spectrometry (GC/MS) analysis of the essential oils of Ajugabracteosa Wall. Ex Benth. and Lavandula dentata L. growing wild in Yemen. J Med Plants Res 6(15):3066–3071

    Google Scholar 

  • Murali ND, Mathela CS (1978) Antifungal study of some indigenous essential oils. Indian Perfum 4:291–292

    Google Scholar 

  • Mimica-Dukić N, Bugarin D, Grbović S, Mitić-Ćulafić D, Vuković-Gačić B, Orčić D, Jovin E, Couladis M (2010) Essential oil of Myrtus communis L. as a potential antioxidant and antimutagenic agents. Molecules 15:2759–2770

    Article  Google Scholar 

  • Ochoa-Velascoa CE, Navarro-Cruza AR, Vera-López O, Paloub E Avila-Sosa R (2018) Growth modeling to control (in vitro) Fusarium verticillioides and Rhizopus stolonifer with thymol and carvacrol. Revista Argentina de Microbiología 50(1):70–74

    Article  Google Scholar 

  • Panizzi L, Flamini G, Cioni PL, Morelli I (1993) Composition and antimicrobial properties of essential oils of four Mediterranean Lamiaceae. J Ethnopharmacol 39(1993):167–170

    Article  Google Scholar 

  • Pavela R (2009) Larvicidal property of essential oils against Culex quinquefasciatus Say (Diptera: Culicidae). Ind Crops Prod 30:311–315

    Article  Google Scholar 

  • Perry NB, Anderson RE, Brennan NJ, Douglas MH, Heaney AJ, McGrimpsey JA, Smallfield BM (1999) Essential oil from Dalmation sage (Salvia officinalis L.), variations among individuals, plant parts, seasons and sites. J Agric Food Chem 47:2048–2054

    Article  Google Scholar 

  • Pintore G, Usai M, Bradesi P, Juliano C, Boatto G, Tomi F, Chessa M, Riccardo Cerri R, Casanova J (2002) Chemical composition and antimicrobial activity of Rosmarinus officinalis L. oils from Sardinia and Corsica. Flavour fragr J 17:15–19

    Article  Google Scholar 

  • Pramila DM, Xavier R, Marimuthu K, Kathiresan S, Khoo ML, Senthilkumar M, Sathya K, Sreeramanan S (2012) Phytochemical analysis and antimicrobial potential of methanolic leaf extract of peppermint (Menthapiperita). J Med Plants Res 6:331–335

    Article  Google Scholar 

  • Rattanapitigorn P, Arakawa M, Tsuro M (2006) Vanillin enhances the antifungal effect of plant essential oils against Botrytis cinerea. Int J Aromather 16:193–198

    Article  Google Scholar 

  • Santamarina P, Roselló J, Sempere F, Giménez S, Amparo M Blázquez (2015) Commercial Origanum compactum Benth and Cinnamomum zeylanicum Blume essential oils against natural mycoflora in Valencia rice. Nat Prod Res 29(23):2215–2218

    Article  Google Scholar 

  • Sbayou H, Oubrim N, Bouchrif B, Ababou B, Boukachabine K, Amghar S (2014) Chemical composition and antibacterial activity of essential oil of Origanum compactum against foodborne bacteria. Int J Eng Res Technol 3(1):3562–3567

    Google Scholar 

  • Sedra MH (1993a) Caractéristiques morphologiques et culturales de Fusarium oxysporum f.sp. albedinis, agent de la fusariose vasculaire (Bayoud) du Palmier Dattier. Revue Al Awamia (INRA, MAROC) ISSN: 0572-2721, no 83 , pp 209–223

  • Sedra MH (1993b) La Fusariose vasculaire du palmier dattier: Possibilités d’identification du Fusarium oxysporum f.sp. albedinis sur la base de ses caractéristiques morphologiques et culturales en relation avec son pouvoir pathogène. Revue Al Awamia (INRA, MAROC) ISSN: 0572-2721, no 82 , pp 71–87

  • Sedra MH (2003a) Le bayoud du palmier dattier en Afrique du Nord. FAO, RNE/SNEA, Tunis. Editions FAO sur la protection des plantes. Imprimerie Signes, Tunis

  • Sedra MH (2003b) Le palmier dattier base de la mise en valeur des oasis au Maroc. Techniques phoénicicoles et Création d’oasis. Edit INRA Maroc. Imprimerie Al Watania, Marrakech

    Google Scholar 

  • Sedra MH (2003c) Date palm cultivation, characterization and classification of main Mauritanian varieties. AOAD, Al-Khartoum

    Google Scholar 

  • Sedra MH (2005) Caractérisation des clones sélectionnés du palmier dattier et prometteurs pour combattre la maladie du Bayoud. In: Boulanouar B, Kradi C (eds) Actes du Symposium International sur le Développement Durable des Systèmes Oasiens, 8 August to 10 March 2005, Erfoud, Morocco, pp 72–79

  • Sghir F, Touati J, Mouria B, Ouazzani Touhami A, Filali-Maltouf A, El Modafar C, Moukhli A, Benkirane R, Douira A (2016) Variation in pathogenicity of Fusarium oxysporum f. sp. albedinis on two cultures associated with date palm of Moroccan oasis. World J Pharm Life Sci 2(3):56–68

    Google Scholar 

  • Singh UP, Singh HB, Singh RB (1980) The fungicidal effect of neem (Azadirachta indica) extracts on some soil borne pathogens of gram (Cicer arietinum). Mycologia 72(6):1077–1093

    Article  Google Scholar 

  • Tantaoui Elaraki A, Lattaoui N, Errifi A, Benjilali B (1993) Composition and antimicrobial activity of the essential oils of Thymus broussonettii T zygis and T satureioides. J Essent Oil Res 5(1):45–53

    Article  Google Scholar 

  • Tantaoui A, Fernandez D (1993) Comparaison entre Fusarium oxysporum f. sp. albedinis et Fusarium oxysporum des sols de palmeraies par l’étude du polymorphisme de longueur des fragments de restriction (RFLP). Phytopathol Mediterr 1:235–244

    Google Scholar 

  • Velluti A, Sanchis V, Ramos AJ, Egido J, Marln S (2003) Inhibitory effect of cinnamon, clove, lemongrass, oregano and palmarose essential oils on growth and fumonisin B1 production by Fusarium proliferatumin in maize grain. J Food Microbiol 89:145–154

    Article  Google Scholar 

  • Yang VW, Clausen CA (2008) Inhibitory effect of essential oils on decay fungi and mold growth on wood. Proc Am Wood Prot Soc 103:62–70

    Google Scholar 

  • Zyani M, Mortabit D, El Abed S, Remmal A, Koraichi SI (2011) Antifungal activity of five plant essential oils against wood decay fungi isolated from an old house at the medina of Fez. Int Res J Microbiol 2(3):104–108

    Google Scholar 

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Acknowledgements

This research was undertaken as part of a Moroccan PPR2/2016/35 (Project supported by MESRSFC and CNRST) and as part of Portugal–Porto University in the context of Erasmus mobility.

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Authors and Affiliations

  1. Research Team on “Biology, Environment and Sustainable Development”, Department of Matter and Life Sciences, Ecole Normale Superieure, Abdelmalek Essaadi University (UAE), 35900, Tétouan, Morocco

    Abderrahmane Rahmouni, Mustapha Khaddor & Abdelfettah Maouni

  2. Research Team in Plant Biotechnology, Faculty of Science, Abdelmalek Essaadi University, Tetouan, Morocco

    Rabah Saidi

  3. Laboratory of Microbiology, Biological Sciences Department, Faculty of Pharmacy of the University of Porto (FFUP), Porto, Portugal

    Eugénia Pinto

  4. Center of Investigation in Chemistry (CIQ), Faculty of Sciences, University of Porto, Porto, Portugal

    Esteves Da Silva Joaquim Carlos Gomes

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  1. Abderrahmane Rahmouni
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Correspondence to Abdelfettah Maouni.

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Communicated by Philippe Michaud, Chief Editor.

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Rahmouni, A., Saidi, R., Khaddor, M. et al. Chemical composition and antifungal activity of five essential oils and their major components against Fusarium oxysporum f. sp. albedinis of Moroccan palm tree. Euro-Mediterr J Environ Integr 4, 27 (2019). https://doi.org/10.1007/s41207-019-0117-x

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