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Antioxidant and Cytotoxic Activities of Aerial and Underground Parts of Hypericum Scabrum L.

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Abstract

We have studied Hypericum scabrum L. plant which belongs to the Hypericaceae family, naturally growing in Anatolia. The aim of this research is to investigate the chemical composition (total phenol, total flavonoid) of 70% methanol and water extracts prepared from the underground and flowering aerial parts to investigate the antioxidant and cytotoxic activity. In order to determine the antioxidant activities of the extracts, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activities and the inhibition of β-carotene/linoleic acid co-oxidation were studied. In cytotoxicity experiments, 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium (MTT) method was used on human lung cancer cell line (A549), human colon adenocarcinoma cell line (COLO 205) and mouse fibroblast (L929) cell line. When the chemical composition of the extracts was examined, the richest extract in terms of total phenolic content was found to be water extract prepared form the flowering part of the plant, and this extract was evaluated to be more active in DPPH radical scavenging activity test than the other extracts. In the cytotoxicity assays, 70% methanol extracts prepared from the flowering and root parts of the plant showed more inhibitory effects on cell viability than water extracts. Our experimental evidence suggests that these Hypericum extracts may be useful as antioxidant and cytotoxic agents.

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References

  • Abdollahi F, Shafaghat A, Salimi F (2012) Biological activity and a biflavonoid from Hypericum scabrum extracts. J Med Plants Res 6:2131–2135

    Google Scholar 

  • Apaydın YB (2006) Sıcaklık stresi oluşturulan broylerlerde yeme ilave edilen Taraxacum officinale L. ve Hypericum scabrum L. bitki ekstraktlarının bazı biyokimyasal parametreler üzerine etkileri. Atatürk Üniv. Ziraat Fak Derg 47(1):65–70

    Google Scholar 

  • Aras A, Khalid S, Jabeen S, Farooqi AA, Xu B (2018) Regulation of cancer cell signaling pathways by mushrooms and their bioactive molecules: overview of the journey from benchtop to clinical trials. Food Chem Toxicol 119:206–214

    Article  Google Scholar 

  • Ayan AK, Radušienė J, Çirak C, Ivanauskas L, Janulis V (2009) Secondary metabolites of Hypericum scabrum and Hypericum bupleuroides. Pharm Biol 47(9):847–853

    Article  Google Scholar 

  • Baytop T (1989) Türkiyede zehirli bitkiler Bitki Zehirlenmeleri ve Tedavi Yöntemleri. İstanbul Üniversitesi Eczacılık Fakültesi, İstanbul

    Google Scholar 

  • Butt G, Attar R, Tabassum S, Aras A, Qadir MI, Ozbey U, Alaaeddine N, Ozcelik B, Farooqi AA (2017) Regulation of signal transduction cascades by Pterostilbenes in different cancers: is it a death knell for oncogenic pathways. Cell Mol Biol 63(12):5–10

    Article  Google Scholar 

  • Dumlupınar Altınsoy B, Şeker Karatoprak G, Ocsoy I (2019) Extracellular directed AgNPs formation and investigation of their antimicrobial and cytotoxic properties. Saudi Pharm J 27(1):9–16

    Article  Google Scholar 

  • Farhan M, Malik A, Ullah MF, Afaq S, Faisal M, Farooqi AA, Biersack B, Schobert R, Ahmad A (2019) Garcinol sensitizes NSCLC cells to standard therapies by regulating EMT-modulating miRNAs. Int J Mol Sci 20(4):E800. https://doi.org/10.3390/ijms20040800

    Article  Google Scholar 

  • Farooqi AA, Ahmad A (2019) Gaze through the clinical lens: molecular and clinical advancements of botanicals. Future Med Chem 11(2):75–77

    Article  Google Scholar 

  • Farooqi AA, Jabeen S, Attar R, Yaylim I, Xu B (2018) Quercetin-mediated regulation of signal transduction cascades and microRNAs: natural weapon against cancer. J Cell Biochem 119(12):9664–9674

    Article  Google Scholar 

  • Farooqi AA, Qureshi MZ, Khalid S, Attar R, Martinelli C, Sabitaliyevich UY, Nurmurzayevich SB, Taverna S, Poltronieri P, Xu B (2019) Regulation of cell signaling pathways by berberine in different cancers: searching for missing pieces of an incomplete jig-saw puzzle for an effective cancer therapy. Cancers (Basel) 11:478. https://doi.org/10.3390/cancers11040478

    Article  Google Scholar 

  • Ganji A, Salehi I, Nazari M, Taheri M, Komaki A (2017) Effects of Hypericum scabrum extract on learning and memory and oxidant/antioxidant status in rats fed a long-term high-fat diet. Metab Brain Dis 32(4):1255–1265

    Article  Google Scholar 

  • Gyamfi MA, Yonamine M, Aniya Y (1999) Free-radical scavenging action of medicinal herbs from ghana, Thonningia sangu inea on experimentally ınduced liver ınjuries. Gen Pharmacol 32:661–667

    Article  Google Scholar 

  • Keser S, Keser F, Kaygili O, Tekin S, Demir E, Turkoglu I, Turkoglu S, Parlak AE, Yilmaz O, Karatepe M, Sandal S, Kirbag S (2018) Phytochemical compounds and antiradical, antimicrobial, and cytotoxic activities of the extracts from Hypericum scabrum L. Flowers. Nat Prod Res. https://doi.org/10.1080/14786419.2018.1493735

    Google Scholar 

  • Lazarova I, Zengin G, Gevrenova R, Nedialkov P, Aneva I, Aumeeruddy MZ, Mahomoodally MF (2019) A comparative study of UHPLC/Orbitrap MS metabolomics profiles and biological properties of Asphodeline taurica from Bulgaria and Turkey. J Pharm Biomed Anal 168:174–180

    Article  Google Scholar 

  • Lin X, Ozbey U, Sabitaliyevich UY, Attar R, Ozcelik B, Zhang Y, Guo M, Liu M, Alhewairini SS, Farooqi AA (2018) Maslinic acid as an effective anticancer agent. Cell Mol Biol (Noisy-le-grand) 64(10):87–91

    Article  Google Scholar 

  • Llorent-Martínez EJ, Zengin G, Lobine D, Molina-García L, Mollica A, Mahomoodally MF (2018) Phytochemical characterization, in vitro and in silico approaches for three Hypericum species. New J Chem 42(7):5204–5214

    Article  Google Scholar 

  • Lobo V, Patil A, Phatak A, Chandra N (2010) Free radicals, antioxidants and functional foods: impact on human health. Pharmacogn Rev 4(8):118–126

    Article  Google Scholar 

  • Naghibi F, Khalaj A, Mosaddegh M, Malekmohamadi M, Hamzeloo-Moghadam M (2014) Cytotoxic activity evaluation of some medicinal plants, selected from Iranian traditional medicine pharmacopoeia to treat cancer and related disorders. J Ethnopharmacol 155:230–239

    Article  Google Scholar 

  • Öztürk N, Tuncel M, Potoğlu-Erkara İ (2009) Phenolic compounds and antioxidant activities of some Hypericum species: a comparative study with H. perforatum. Pharm Biol 47(2):120–127

    Article  Google Scholar 

  • Qureshi MZ, Attar R, Romero MA, Sabitaliyevich UY, Nurmurzayevich SB, Ozturk O, Wakim LH, Lin X, Ozbey U, Yelekenova AB, Farooqi AA (2019) Regulation of signaling pathways by β-elemene in cancer progression and metastasis. J Cell Biochem. https://doi.org/10.1002/jcb.28624

    Google Scholar 

  • Re R, Pellegrini N, Proteggente A et al (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231–1237

    Article  Google Scholar 

  • Shafaghat A (2011) Antioxidant, antimicrobial activities and fatty acid components of flower, leaf, stem and seed of Hypericum scabrum. Nat Prod Commun 6:1739–1742

    Google Scholar 

  • Singleton VL, Orthofer R, Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol 299:152–178

    Article  Google Scholar 

  • Uysal A, Gunes E (2015) Antimicrobial and anti-MRSA effects of three extracts of some Hypericum species against standard microorganisms and methicillin resistant Staphylococcus aureus (MRSA) Strains. Curr Bioact Compd 11:146–151

    Article  Google Scholar 

  • Uysal S, Senkardes I, Mollica A, Zengin G, Bulut G, Dogan A, Glamoclija J, Sokovic M, Lobine D, Mahomoodally FM (2018) Biologically active compounds from two members of the Asteraceae family: Tragopogon dubius Scop. and Tussilago farfara L. J Biomol Struct Dyn 24:1–13

  • Vafi F, Bahramsoltani R, Abdollahi M, Manayi A, Abdolghaffari AH, Samadi N, Khanavi M (2016) Burn wound healing activity of Lythrum salicaria L. and Hypericum scabrum L. Wounds 28(12):448–458

    Google Scholar 

  • Velioğlu YS, Mazza G, Oomah BD (1998) Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 46:4113–4117

    Article  Google Scholar 

  • Zengin G, Bulut G, Mollica A, Picot-Allain CMN, Mahomoodally MF (2018a) In vitro and in silico evaluation of Centaurea saligna (K. Koch) Wagenitz—An endemic folk medicinal plant. Comput Biol Chem 73:120–126

    Article  Google Scholar 

  • Zengin G, Rodrigues MJ, Abdallah HH, Custodio L, Stefanucci A, Aumeeruddy MZ, Mollica A, Rengasamy KRR, Mahomoodally MF (2018b) Combination of phenolic profiles, pharmacological properties and in silico studies to provide new insights on Silene salsuginea from Turkey. Comput Biol Chem 77:178–186

    Article  Google Scholar 

  • Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555–559

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacognosy, Faculty of Pharmacy, Erciyes University, 38039, Kayseri, Turkey

    Gökçe Şeker Karatoprak, Hasan Çağrı Kaytan & Esra Köngül Şafak

  2. Ziya Eren Drug Application and Research Center, Erciyes University, Kayseri, Turkey

    Gökçe Şeker Karatoprak & Çiğdem Yücel

  3. Department of Pharmaceutical Technology, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey

    Çiğdem Yücel

  4. Department of Pharmaceutical Botany, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey

    Selen İlgün

  5. Department of Pharmacognosy, Faculty of Pharmacy, Eastern Mediterranean University, Via Mersin-10, Famagusta, North Cyprus, Turkey

    Müberra Koşar

Authors
  1. Gökçe Şeker Karatoprak
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  2. Çiğdem Yücel
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  3. Hasan Çağrı Kaytan
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  4. Selen İlgün
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  5. Esra Köngül Şafak
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  6. Müberra Koşar
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Correspondence to Gökçe Şeker Karatoprak.

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Şeker Karatoprak, G., Yücel, Ç., Kaytan, H.Ç. et al. Antioxidant and Cytotoxic Activities of Aerial and Underground Parts of Hypericum Scabrum L.. Iran J Sci Technol Trans Sci 43, 2107–2113 (2019). https://doi.org/10.1007/s40995-019-00717-1

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  • DOI: https://doi.org/10.1007/s40995-019-00717-1

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