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Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina

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Abstract

In Argentina, more than 60 % of the tobacco crops are grown in the northwestern part of the country and where Rhizoctonia solani leads to a reduction in crop yield and quality. In this study, 35 isolates of Rhizoctonia were obtained from 32 tobacco fields in northwestern Argentina and characterized by both morphological and molecular approaches. Based on the variability in the ITS region, isolates were identified as R. solani (80 %), Waitea circinata var. zeae (Rhizoctonia zeae) (8 %) and binucleate Rhizoctonia (8 %). Most isolates of R. solani belonged to the anastomosis groups (AGs) AG 4 HG-I (44 %), AG 2-1 (41 %) and AG 4 HG-III (13 %). Isolates of binucleate Rhizoctonia belonged to AG-F and AG-P of Ceratobasidium sp. Morphological variability was higher within isolates of AG 2-1 and AG 4 HG-III than within those of AG 4 HG-I. Aggressiveness of the isolates towards tobacco seedlings was assessed in the greenhouse. Isolates of AG 2-1 were the most aggressive on leaves, causing target spot, whereas isolates of AG 4 HG-I were the most aggressive on stems and roots, causing damping-off.

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Acknowledgments

We thank C. Sanchez for statistical advice. Thanks are also given to Instituto Nacional de Tecnología Agropecuaria for providing a doctorate fellowship for the first author and support by means of research grants (PNIN082511 and PRSalJu09).

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Authors and Affiliations

  1. Instituto Nacional de Tecnología Agropecuaria (INTA) EEA Salta, Ruta Nac. 68 Km 172 (4403) Cerrillos, Salta, Argentina

    Guadalupe E. Mercado Cárdenas & Marta Z. Galván

  2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), CCT-Salta. Av. Bolivia 5150 (4400), Salta, Argentina

    Marta Z. Galván

  3. IMYZA, INTA Castelar, Buenos Aires, Argentina

    Viviana A. Barrera

  4. Laboratorio de Genética Evolutiva, Departamento de Ecología, Genética y Evolución, UBA, Buenos Aires, Argentina

    Marcela S. Rodriguero

  5. FAUBA, Buenos Aires, Argentina

    Marcelo A. Carmona

  6. IPAVE, INTA, Córdoba, Argentina

    Guillermo J. March

  7. UNT, Tucumán, Argentina

    Ana C. Ramallo

  8. Department of Plant Pathology, North Carolina State University, Raleigh, NC, 27695, USA

    H. David Shew

Authors
  1. Guadalupe E. Mercado Cárdenas
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  2. Marta Z. Galván
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  3. Viviana A. Barrera
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  4. Marcela S. Rodriguero
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  5. Marcelo A. Carmona
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  6. Guillermo J. March
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  7. Ana C. Ramallo
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  8. H. David Shew
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Correspondence to Guadalupe E. Mercado Cárdenas.

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Section Editor: Adalberto Café-Filho

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Mercado Cárdenas, G.E., Galván, M.Z., Barrera, V.A. et al. Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina. Trop. plant pathol. 40, 160–168 (2015). https://doi.org/10.1007/s40858-015-0035-7

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  • DOI: https://doi.org/10.1007/s40858-015-0035-7

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