Abstract
Botrytis bunch rot, caused by Botrytis cinerea, is one of the most destructive diseases of grapes (Vitis vinifera L.). A phenotyping assay was designed under laboratory conditions to analyze grape-B. cinerea interactions. A conidial suspension of concentration 1 × 105 conidia/ml was prepared and used to inoculate berries from two different grape varieties: V. aestivalis-derived ‘Norton’, a North American hybrid, and V. vinifera ‘Cabernet Sauvignon’, a European grape. The differences in the infection reaction between these two cultivars were visible 10 days after inoculation. Berries from Norton had a low average disease incidence (7.5 %) and disease severity (3.7 %). In contrast, berries from Cabernet Sauvignon were completely susceptible, exhibiting an average disease incidence and severity greater than 90 %. This phenotyping assay can be further applied to evaluate disease progression in a mapping population, developed by crossing V. aestivalis-derived ‘Norton’ and V. vinifera ‘Cabernet Sauvignon’.
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Acknowledgments
We thank James English for providing metallic racks and technical support. We also thank Marilyn Odneal and Kevin Fort for valuable discussions and constructive comments on the manuscript. This work was supported by Agriculture and Food Research Initiative Competitive Grant no. 2013-67014-21360 from the USDA National Institute of Food and Agriculture.
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Section Editor: Adalberto Café
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Sapkota, S., Chen, LL., Schreiner, K. et al. A phenotypic study of Botrytis bunch rot resistance in Vitis aestivalis-derived ‘Norton’ grape. Trop. plant pathol. 40, 279–282 (2015). https://doi.org/10.1007/s40858-015-0028-6
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DOI: https://doi.org/10.1007/s40858-015-0028-6