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Light-emitting diodes (LED) increase the stomata formation and chlorophyll content in Musa acuminata (AAA) ‘Nanicão Corupá’ in vitro plantlets

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Abstract

Micropropagation of banana by means of different morphogenetic routes is an important source of genetically uniform, pest- and disease-free planting materials of Musa spp. Morphogenesis of in vitro grown plants are affected by light quality, light quantity and photoperiod. Light-emitting diode (LED) lighting systems have several unique advantages, including their peak spectral output, which closely coincides with the red absorption peak of chlorophyll and the reported wavelengths for maximum photosynthetic efficiency. The present study was aimed to investigate the effect of two LED lighting treatments (white LED and deep red/white LED) compared to conventional fluorescent lamps on the stomata formation and chlorophyll (Chl) levels in micropropagated banana plantlets through organogenesis. Both LED lighting evaluated increased the levels of total Chl, Chl-a and Chl-b in banana in vitro plantlets, indicating no statistical difference between them, but being higher then plantlets subjected to fluorescent lamps. The number of stomata was also increased equally by both LED lighting treatments, indicating increased formation of stomata in both abaxial and adaxial leaf surfaces when compared to fluorescent lamps. Although all treatments presented 100 % survival in the acclimatization phase, LED lighting show promising for micropropagation of banana due to its known superior light quality, low energy consumed, little heat generated and long operating lifetime.

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References

  • Bairu MW, Stirk WA, Doležal K, Van Staden J (2007) Optimizing the micropropagation protocol for the endangered Aloe polyphylla: can meta-topolin and its derivatives serve as replacement for benzyladenine and zeatin? Plant Cell, Tissue Organ Cult 90:15–23

    Article  CAS  Google Scholar 

  • Bairu MW, Stirk WA, Doležal K, Van Staden J (2008) The role of topolins in micropropagation and somaclonal variation of banana cultivars ‘Williams’ and ‘Grand Naine’ (Musa spp. AAA). Plant Cell, Tissue Organ Cult 95:373–379

    Article  CAS  Google Scholar 

  • Banerjee N, Vuylsteke DR, De Langhe EAL (1986) Meristem tip culture of Musa: histomorphological studies of shoot bud proliferation. In: Withers LA, Alderson PG (eds) Plant tissue culture and its agricultural applications. Butterworths, London, pp 139–147

    Chapter  Google Scholar 

  • Baroja-Fernandez E, Aguirreolea J, Martinkova H, Hanus J, Strnad M (2002) Aromatic cytokinins in micropropagated potato plants. Plant Physiol Biochem 40:217–224

    Article  CAS  Google Scholar 

  • Bhagyalakshmi N, Singh NS (1995) Role of liquid versus agar-gelled media in mass propagation and ex vitro survival in bananas. Plant Cell, Tissue Organ Cult 41:71–73

    Article  Google Scholar 

  • Bogaert I, van Cauter S, Werbrouck SPO, Doležal K (2006) New aromatic cytokinins can make the difference. Acta Hortic 725:265–270

    CAS  Google Scholar 

  • Briggs WR, Olney MA (2001) Photoreceptors in plant photomorphogenesis to date, five photochromes, two cryptochrome, one phototropin and one superchrome. Plant Physiol 125:85–88

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Clouse SD (2001) Integration of light and brassinosteroid signals in etiolated seedling growth. Trends Plant Sci 6:443–445

    Article  CAS  PubMed  Google Scholar 

  • Cronauer S, Krikorian AD (1983) Somatic embryos from cultured tissues of triploid plantains (Musa‘ABB’). Plant Cell Rep 2:289–291

    Article  CAS  PubMed  Google Scholar 

  • Cronauer S, Krikorian AD (1988) Plant regeneration via somatic embryogenesis in the seeded diploid banana Musa ornata Roxb. Plant Cell Rep 7:23–25

    Article  Google Scholar 

  • Dewir YH, Chakrabarty D, Hahn EJ, Paek KY (2006) The effects of paclobutrazol, light emitting diodes (LEDs) and sucrose on flowering of Euphorbia millii plantlets in vitro. Eur J Hortic Sci 71:240

    CAS  Google Scholar 

  • Dhed’a D, Dumortier F, Panis B, Vuylsteke D, De Langhe E (1991) Plant regeneration in cell suspension cultures of cooking banana ‘Bluggoe’ cultivar (Musa spp. ABB group). Fruits 46:125–135

    Google Scholar 

  • Escalant JV, Teisson C (1989) Somatic embryogenesis and plants from immature zygotic embryos of the species Musa acuminata and Musa bulbisiana. Plant Cell Rep 7:665–668

    CAS  PubMed  Google Scholar 

  • Heo J, Lee C, Chakrabarty D, Paek K (2002) Growth responses of marigold and salvia bedding plants as affected by monochromic or mixture radiation provided by a light emitting diode (LED). Plant Growth Regul 38:225–230

    Article  CAS  Google Scholar 

  • Jao RC, Fang W (2004) Growth of potato plantlets in vitro is different when provided concurrent versus alternating blue and red light photoperiods. HortScience 39:380–382

    Google Scholar 

  • Jao RC, Lai CC, Fang W, Chang SF (2005) Effects of red light on the growth of Zantedeschia plantlets in vitro and tuber formation using light-emitting diodes. HortScience 40(2):436–438

    Google Scholar 

  • Kim SJ, Hahn EJ, Heo JW, Paek KY (2004) Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Sci Hortic 101:143–151

  • Kozai T, Smith MAL (1995) Environmental control in plant tissue culture—general introduction and overview. In: Aitken-Christie J, Kozai J, Smith MAL (eds) Automation and environmental control in plant tissue culture. Kluwer Academic Publishers, Dordrecht, pp 301–318

    Chapter  Google Scholar 

  • Kurilčik A, Miklušytė-Čanova R, Dapkūnienė S, Žilinskaitė S, Kurilčik G, Tamulaitis PD, Žukauskas A (2008) In vitro culture of Chrysanthemum plantlets using light-emitting diodes. Cent Eur J Biol 3:161–167

    Google Scholar 

  • Lee SH, Tewari RK, Hahn EJ, Paek KY (2007) Photon flux density and light quality induce changes in growth, stomatal development, photosynthesis and transpiration of Withania somnifera (L.) Dunal. plantlets. Plant Cell, Tissue Organ Cult 90:141–151

    Article  CAS  Google Scholar 

  • Li H, Xu Z, Tang C (2010) Effect of light-emitting diodes on growth and morphogenesis of upland cotton (Gossypium hirsutum L.) plantlets in vitro. Plant Cell, Tissue Organ Cult 103:155–163

    Article  Google Scholar 

  • Martin KP, Pachathundikandi S, Zhang CL, Slater A, Madassery J (2006) RAPD analysis of a variant of banana (Musa sp.) cv. Grande naine and its propagation via shoot tip culture. In Vitro Cell Dev Biol Plant 42:188–192

    Article  CAS  Google Scholar 

  • Martins M, Sarmento D, Oliveira MM (2004) Genetic stability of micropropagated almond plantlets as assessed by RAPD and ISSR markers. Plant Cell Rep 23:492–496

    Article  CAS  PubMed  Google Scholar 

  • Moon HK, Park SK, Kim YW, Kim CS (2006) Growth of Tsuru-rindo (Tripterospermum japonicum) cultured in vitro under various sources of light-emitting diode (LED) irradiation. J Plant Biol 49:174–179

    Article  Google Scholar 

  • Morel GM, Wetmore RH (1951) Tissue culture of monocotyledons. Am J Bot 38:138–140

    Article  CAS  Google Scholar 

  • Moshe R, Dalia E (2007) On the effect of light on shoot regeneration in petunia. Plant Cell, Tissue Organ Cult 89:49–54

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Navarro C, Escobedo RM, Mayo A (1997) In vitro plant regeneration from embryonic cultures of a diploid and a triploid, Cavendish banana. Plant Cell, Tissue Organ Cult 51:17–25

    Article  Google Scholar 

  • Nhut DT, Takamura T, Watanabe H, Tanaka M (2001) Efficiency of a novel culture system by using light-emitting diode (LED) on in vitro and subsequent growth of micropropagated banana plantlets. Acta Hortic 616:121–127

    Google Scholar 

  • Nhut DT, Takamura T, Watanabe H, Okamoto K, Tanaka M (2003) Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs). Plant Cell, Tissue Organ Cult 73:43–52

    Article  CAS  Google Scholar 

  • Nhut DT, Takamura T, Watanabe H, Okamoto K, Tanaka M (2005) Artificial light source using light-emitting diodes (LEDs) in the efficient micropropagation of Spathiphyllum plantlets. Acta Hortic 692:137–142

    Google Scholar 

  • Poudel PR, Tamura H, Kataoka I, Mochioka R (2008) Phenolic compounds and antioxidant activities of skins and seeds of five wild grapes and two hybrids native to Japan. J Food Compost Anal 21:622–625

    Article  CAS  Google Scholar 

  • Ray T, Dutta I, Saha P, Das S, Roy SC (2006) Genetic stability of three economically important micropropagated banana (Musa spp.) cultivars of lower Indo-Gangetic plains, as assessed by RAPD and ISSR markers. Plant Cell, Tissue Organ Cult 85:11–21

    Article  CAS  Google Scholar 

  • Schuerger AC, Brown CS, Stryjewski EC (1997) Anatomical features of pepper plants (Capsicum annuum L.) grown under red light-emitting diodes supplemented with blue or far-red light. Ann Bot 79:273–282

  • Shin KS, Murthy HN, Heo JW, Hahn EJ, Paek KY (2008) The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plants. Acta Physiol Plant 30:339–343

    Article  CAS  Google Scholar 

  • Topchiy NM, Sytnik SK, Syvash OO, Zolotareva OK (2005) The effect of additional red irradiation on the photosynthetic apparatus of Pisum sativum. Photosynthetica 43:451–456

    Article  CAS  Google Scholar 

  • Tripathy BC, Brown CS (1995) Root-shoot interaction in the greening of wheat seedlings grown under red light. Plant Physiol 107:407–411

    CAS  PubMed Central  PubMed  Google Scholar 

  • Venkatachalam L, Sreedhar RV, Bhagyalakshmi N (2007) Genetic analyses of micropropagated and regenerated plantlets of banana as assessed by RAPD and ISSR markers. In Vitro Cell Dev Biol Plant 43:267–274

    Article  CAS  Google Scholar 

  • Wellburn AR (1994) The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol 144:307–313

    Article  CAS  Google Scholar 

  • Werbrouck SPO, Strnad M, van Onckelen HA, Debergh PC (1996) Meta-topolin, an alternative to benzyladenine in tissue culture. Physiol Plant 98:291–297

    Article  CAS  Google Scholar 

  • Wilken D, Gonzalez EJ, Gerth A, Gómez-Kosky R, Schumann A, Claus D (2014) Effect of immersion systems, lighting, and TIS designs on biomass increase in micropropagating banana (Musa spp. cv’.Grande naine’AAA). In Vitro Cell Dev Biol Plant 50:582–589

    Article  CAS  Google Scholar 

  • Wongnok A, Piluek C, Techasilpitak T, Tantivivat S (2008) Effects of light emitting diodes on micropropagation of Phalaenopsis orchids. Acta Hortic 788:149–156

    Google Scholar 

  • Zeiger E (1984) Blue light and stomatal function. In: Senger H (ed) Blue light effects in biological systems. Springer, Berlin, pp 484–494

    Chapter  Google Scholar 

Download references

Acknowledgments

This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Fundação de Amparo a Pesquisa e Inovação do Estado de Santa Catarina (FAPESC). The authors thank Dr. Gilmar Roberto Zaffari (EPAGRI) for providing banana plants.

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    1. Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Universidade Federal de Santa Catarina, Rodovia Admar Gonzaga, Km 3, Florianópolis, SC, 88034-001, Brazil

      Leila do Nascimento Vieira, Hugo Pacheco de Freitas Fraga, Karina Goulart dos Anjos, Catarina Corrêa Puttkammer, Ramon Felipe Scherer, Dorival Almeida da Silva & Miguel Pedro Guerra

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    1. Leila do Nascimento Vieira
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    2. Hugo Pacheco de Freitas Fraga
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    Correspondence to Miguel Pedro Guerra.

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    Leila do Nascimento Vieira and Hugo Pacheco de Freitas Fraga have contributed equally to this work.

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    do Nascimento Vieira, L., de Freitas Fraga, H.P., dos Anjos, K.G. et al. Light-emitting diodes (LED) increase the stomata formation and chlorophyll content in Musa acuminata (AAA) ‘Nanicão Corupá’ in vitro plantlets. Theor. Exp. Plant Physiol. 27, 91–98 (2015). https://doi.org/10.1007/s40626-015-0035-5

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