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Fas/FasL gene polymorphism in patients with Hashimoto’s thyroiditis in Turkish population

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Abstract

Objective

Hashimoto’s disease is a polygenic disorder with complex etiopathogenesis. Apoptosis is proposed as one of its mechanisms. The Fas/Fas ligand cascade represents a major pathway initiating apoptosis. This study aims to evaluate the influence of Fas and FasL gene polymorphism in Hashimoto’s thyroiditis in Turkish population.

Materials and methods

A total of 112 patients with Hashimoto’s thyroiditis and 112 cases of healthy control people were included in this study. The evaluation of genotype for Fas -670 A/G and FasL 843 C/T gene polymorphism was performed by using PCR-RFLP method.

Results

The FAS genotype and gene allele frequency distribution did differ between the control group (AA 36.6 %, AG 50.0 %, GG 13.4 %, A 61.6 %, G 38.4 %) and the Hashimoto’s thyroiditis patients (AA 21.4 %, AG 50.9 %, GG 27.7 %, A 46.9 %, G 53.1 %) (p < 0.01). The evaluation of FasL genotype and gene allele frequency did not show statistically significant difference between the patient group (CC 27.7 %, CT 45.5 %, TT 26.8 %, C 50.4 %, T 49.6 %) and control group (CC 33.9 %, CT 44.6 %, TT 21,4 %, C 56.3 %, T 43.8 %) (p > 0.05).

Conclusions

Gene polymorphism of Fas and G allele frequency may play a role in the regulation of apoptosis in thyroid autoimmune disorders. There is a need for further studies to clarify the genetic role of apoptosis in HT.

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Authors and Affiliations

  1. Department of Endocrinology and Metabolic Diseases, Ege University Medical School, 35100, Bornova, Izmir, Turkey

    M. Erdogan

  2. Department of Endocrinology and Metabolic Diseases, Necmettin Erbakan University Medical School, Konya, Turkey

    M. Kulaksizoglu

  3. Department of Internal Medicine, Numune Training and Research Hospital, Adana, Turkey

    S. Ganidagli

  4. Laboratory of Molecular Medicine, Department of Pediatrics, Ege University Medical School, Bornova, Izmir, Turkey

    A. Berdeli

Authors
  1. M. Erdogan
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  2. M. Kulaksizoglu
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  3. S. Ganidagli
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  4. A. Berdeli
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Correspondence to M. Erdogan.

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The authors declare no conflict of interest.

Ethical approval

Consent forms and protocols were approved by the Ethical Committee of Adana Numune Education and Research Hospital. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the authors.

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Informed consent was obtained from all individual participants included in the study.

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Erdogan, M., Kulaksizoglu, M., Ganidagli, S. et al. Fas/FasL gene polymorphism in patients with Hashimoto’s thyroiditis in Turkish population. J Endocrinol Invest 40, 77–82 (2017). https://doi.org/10.1007/s40618-016-0534-5

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  • DOI: https://doi.org/10.1007/s40618-016-0534-5

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