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Tupistra nutans Wall. root extract, rich in phenolics, inhibits microbial growth and α-glucosidase activity, while demonstrating strong antioxidant potential

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Abstract

The current study was performed to determine the phenolic compounds composition, antioxidant and antimicrobial activity, and α-glucosidase inhibitory effect of the root extract of Tupistra nutans Wall., in order to validate its use in traditional medicine. HPLC–MS/MS analysis showed the presence of ferulic acid, protocatechuic acid, p-hydroxybenzoic acid, p-coumaric acid, salicylic acid, chlorogenic acid, caffeic acid, l-phenylalanine as the predominant phenolic compounds. The antioxidant potentials of T. nutans were determined in different solvent extract and found to vary in a concentration-dependent manner. Ethyl acetate-soluble fraction exhibited the highest amount of phenolic acids, flavonoids, antioxidant activity against the DPPH, reduced Fe3+/ferric cyanide complexes to the ferrous form and inhibitory effect on α-glucosidase. Butanol fraction showed the strongest ABTS radical scavenging ability. Methanolic root extract showed higher reduction capability of ferric ions than the other solvent fractions. A significant and higher positive correlation was found between total phenolic content and total flavonoid content with α-glucosidase inhibition and DPPH using ethyl acetate solvent than the other solvent fractions. All the tested microorganisms: Staphylococcus aureus Rosenback (ATCC13150), Salmonella enterica typhimurium Kauffmann and Edwads (ATCC14028), Escherichia coli Castllani and Chalmers (ATCC35150), and fungi: Candida albicans Robin and Berkhout (KTCC7965), Aspergillus fumigatus Fresenius (KTCC6145), A. flavus var. flavus Link ex Fries (KTCC6143), A. niger van Tieghem (KTCC6317) were susceptible to the root extracts at a concentration of 0.12–0.25 mg mL−1. This study may allow us to understand indigenous medicinal values of T. nutans. Furthermore, these results showed that T. nutans is a good source of antioxidants along with antimicrobial and antidiabetic activities and could be used as an important bioresource for the pharmaceutical and food industries.

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Acknowledgements

This study was supported by the KU Research Professor program.

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Authors and Affiliations

  1. Department of Applied Life Science, Konkuk University, Seoul, 143-701, South Korea

    Ill-Min Chung, Seung-Hyun Kim & Bimal Kumar Ghimire

  2. Bioherb Research Institute, Kangwon National University, Chuncheon, 200-701, South Korea

    Chang Yeon Yu

  3. Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, 200-701, South Korea

    Ramachandran Chelliah & Deog-Hwan Oh

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  1. Ill-Min Chung
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  2. Ramachandran Chelliah
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  4. Seung-Hyun Kim
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BKG performed designing of experiment and writing of manuscript. CYY supervised the experiment. RC and D-HO performed the antimicrobial activities. S-HK and I-MC performed the phytochemical determination, analysis and editing of manuscript.

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Correspondence to Bimal Kumar Ghimire.

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Chung, IM., Chelliah, R., Oh, DH. et al. Tupistra nutans Wall. root extract, rich in phenolics, inhibits microbial growth and α-glucosidase activity, while demonstrating strong antioxidant potential. Braz. J. Bot 42, 383–397 (2019). https://doi.org/10.1007/s40415-019-00547-w

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