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Desiccation tolerance in bryophytes: the rehydration proteomes of Bryum argenteum provide insights into the resuscitation mechanism

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Abstract

Bryum argenteum Hedw. is a desiccation tolerant bryophyte and belongs to one of the most important components of the biological soil crusts (BSCs) found in the deserts of Central Asia. Limited information is available on rehydration-responsive proteins in desiccation tolerant plants. As a complement to our previous research analyzing the rehydration transcriptome, we present a parallel quantitative proteomic effort to study rehydration-responsive proteins. Bryophyte gametophores were desiccated (Dry) and rehydrated for 2 h (R2) and 24 h (R24). Proteins from Dry, R2 and R24 gametophores were labeled by isobaric tags for relative and absolute quantitation (iTRAQ) to determine the relative abundance of rehydration-responsive proteins. A total of 5503 non-redundant protein sequences were identified and 4772 (86.7%) protein sequences were annotated using Gene Ontology (GO) terms and Pfam classifications. Upon rehydration 239 proteins were elevated and 461 proteins were reduced as compared to the desiccated protein sample. Differentially up-regulated proteins were classified into a number of categories including reactive oxygen species scavenging enzymes, detoxifying enzymes, Late Embryogenesis Abundant (LEA) proteins, heat shock proteins, proteasome components and proteases, and photosynthesis and translation related proteins. Furthermore, the results of the correlation between transcriptome and proteome revealed the discordant changes in the expression between protein and mRNA.

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Acknowledgements

This research was financially supported by the Scientific Service Project of Chinese Academy of Sciences (TSS-2015-014-FW-4-3) and the National Science Foundation of China-Xinjiang Talent Youth Project (U1403302).

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Authors and Affiliations

  1. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, China

    Bei Gao, Daoyuan Zhang, Xiaoshuang Li, Honglan Yang, Yuqing Liang & Yuanming Zhang

  2. School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China

    Bei Gao, Moxian Chen & Jianhua Zhang

  3. Department of Biology, Faculty of Science, Hong Kong Baptist University, Hong Kong, China

    Jianhua Zhang

  4. Department of Plant Biology, Southern Illinois University-Carbondale, Carbondale, IL, 62901-6509, USA

    Wood Andrew

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  1. Bei Gao
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  2. Daoyuan Zhang
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  3. Xiaoshuang Li
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  4. Honglan Yang
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  9. Wood Andrew
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Correspondence to Daoyuan Zhang.

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Gao, B., Zhang, D., Li, X. et al. Desiccation tolerance in bryophytes: the rehydration proteomes of Bryum argenteum provide insights into the resuscitation mechanism. J. Arid Land 10, 152–167 (2018). https://doi.org/10.1007/s40333-017-0033-3

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  • DOI: https://doi.org/10.1007/s40333-017-0033-3

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