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High Resolution Melting Analysis is Very Useful to Identify Breast Cancer Type 1 Susceptibility Protein (BRCA1) c.4964_4982del19 (rs80359876) Founder Calabrian Pathogenic Variant on Peripheral Blood and Buccal Swab DNA

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An Erratum to this article was published on 07 March 2017

Abstract

Introduction

Detection of pathogenic variants in hereditary breast and ovarian cancer-related breast cancer type 1 and type 2 susceptibility proteins (BRCA1/2) genes is an effective strategy in cancer prevention and treatment. Some ethnic and geographical regions show different BRCA1/2 mutation spectrum and prevalence. In Italy, elucidation of founder effect in BRCA1/2 genes can have an impact on the management of hereditary cancer families on a healthcare system level, making genetic testing more affordable and cost effective in certain regions.

Methods

The purpose of this paper is to develop a rapid, low-cost, high-throughput single-tube technology for genotyping the Italian founder mutation c.4964_4982del19 (rs80359876) in the BRCA1 gene, starting from peripheral blood and/or buccal swab DNA.

Results

Heterozygote samples for c.4964_4982del19 variant were easily and unambiguously identified by the altered shape of the melting curves and were clearly distinguished by a change in melting temperature that differed by approximately 5 °C. The same results were obtained both with DNA from peripheral blood than buccal swab.

Conclusions

We provide evidence about application of high-resolution melting analysis (HRMA) in unambiguously genotyping of the founder BRCA1 c.4964_4982del19 variant (rs80359876) in individuals from the Calabria region of Italy. In fact, HRMA was confirmed to be particularly suitable for the identification of BRCA1 c.4964_4982del19 variant, making this approach useful in clinical molecular diagnostics.

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Authors and Affiliations

  1. Laboratory of Clinical Molecular and Personalized Diagnostics, Institute of Biochemistry and Clinical Biochemistry, Teaching and Research Hospital ‘Agostino Gemelli’ Foundation, Rome, Italy

    Angelo Minucci, Maria De Bonis, Elisa De Paolis, Leonarda Gentile, Concetta Santonocito, Paola Concolino, Flavio Mignone & Ettore Capoluongo

Authors
  1. Angelo Minucci
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  2. Maria De Bonis
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  3. Elisa De Paolis
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  4. Leonarda Gentile
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  5. Concetta Santonocito
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  6. Paola Concolino
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  7. Flavio Mignone
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  8. Ettore Capoluongo
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Corresponding authors

Correspondence to Angelo Minucci or Ettore Capoluongo.

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Conflict of interest

The authors (AM, MDB, EDP, LG, CS, PC, FM and EC) declare they have no competing interests.

Funding

No funding to declare.

Ethical approval and informed consent

The study was performed ensuring compliance of human studies with the Helsinki Declaration.

Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/s40291-017-0273-0.

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Minucci, A., De Bonis, M., De Paolis, E. et al. High Resolution Melting Analysis is Very Useful to Identify Breast Cancer Type 1 Susceptibility Protein (BRCA1) c.4964_4982del19 (rs80359876) Founder Calabrian Pathogenic Variant on Peripheral Blood and Buccal Swab DNA. Mol Diagn Ther 21, 217–223 (2017). https://doi.org/10.1007/s40291-017-0262-3

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  • DOI: https://doi.org/10.1007/s40291-017-0262-3

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