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Rapid quantification of astilbin in rat plasma by liquid chromatography-tandem mass spectrometry and its application to pharmacokinetic study

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Abstract

Astilbin is a potential immunosuppressive agent with minor cytotoxicity. Its oral bioavailability is supposed to be rather low and therefore a sensitive analytical method is required for its pharmacokinetic study after oral administration. A simple, sensitive and rapid liquid chromatography-tandem mass spectrometry(LC-MS/MS) method was developed and validated for the determination of astilbin in rat plasma. Plasma samples were subjected to liquid-liquid extraction with ethyl acetate and separated by reversed phase high performance liquid chromatography(HPLC) with methanol-0.01%(volume fraction) formic acid(50:50, volume ratio) as mobile phase. Quantitive determination was achieved on negative LC-MS/MS by a multiple reaction moitoring method with transitions m/z 449.1→150.9(quantifier) and m/z 449.1→284.9(qualifier) for astilbin and m/z 128.9→42.0 for internal standard(IS). A lower limit of quantification(LLOQ) of ng/mL was achieved within a short cycle time of 3.4 min. The method was successfully applied to a pharmacokinetic study involving oral and intravenous administrations of 6 mg/kg astilbin to six rats.

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Authors and Affiliations

  1. School of Life Sciences, Jilin University, Changchun, 130012, P. R. China

    Lei Yin, Yun-hui Zhang, Sen Zhao, Long-mei Cheng, Mei-yun Shi, Yan Yang & Xi-dong Liu

  2. Clinical Pharmacology Center, Research Institute of Translational Medicine, the First Hospital of Jilin University, Changchun, 130061, P. R. China

    Lei Yin & Yan-tong Sun

  3. National Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun, 130012, P. R. China

    Yan Yang

  4. Key Laboratory for Molecular Enzymology and Engineering, the Ministry of Education, Jilin University, Changchun, 130012, P. R. China

    Yan Yang

  5. School of Pharmaceutical Sciences, Jilin University, Changchun, 130021, P. R. China

    Yan-tong Sun

  6. School of Pharmacy, University of Otago, Dunedin, 9054, New Zealand

    J. Paul Fawcett

Authors
  1. Lei Yin
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  2. Yun-hui Zhang
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  3. Sen Zhao
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  4. Long-mei Cheng
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  5. Mei-yun Shi
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  6. Yan Yang
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  7. Yan-tong Sun
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  8. Xi-dong Liu
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  9. J. Paul Fawcett
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Corresponding authors

Correspondence to Yan Yang or Yan-tong Sun.

Additional information

Supported by the National Natural Science Foundation of China(No.81102383), the Science and Technology Major Specialized Projects for “Significant New Drugs Creation” of the 12th Five-year Plan of China(No.2012ZX09303-015) and the National Key Technology R&D Program of the Ministry of Science and Technology, China(No.2012BAI30B00).

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Yin, L., Zhang, Yh., Zhao, S. et al. Rapid quantification of astilbin in rat plasma by liquid chromatography-tandem mass spectrometry and its application to pharmacokinetic study. Chem. Res. Chin. Univ. 29, 1078–1082 (2013). https://doi.org/10.1007/s40242-013-3166-8

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  • DOI: https://doi.org/10.1007/s40242-013-3166-8

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