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Green Silver Nanoparticles for Phytopathogen Control

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Plant diseases bring radical problem in the agriculture sector. Phytopathogens mediate diseases that pose considerable loss of yield and quality deterioration which eventually bring down the crop yield and the rural economy. The present study is, thus, focused on developing the optimized protocol for the synthesis of silver nanoparticles (AgNPs) by green chemistry approach and revealing their antimicrobial potential against phytopathogens. The synthesis of AgNPs was carried out by using aqueous plant extracts of three medicinal and aromatic plants, namely Allium cepa (onion), Allium sativum (garlic) and Zingiber officinale (ginger). AgNPs were characterized by various analytical techniques including UV–visible spectra, PSA, FTIR, TEM and XRD analysis. The AgNPs were spherical with size ranging from 1 to 10 nm, crystalline in nature and relatively stable up to 3 months after synthesis. The AgNPs conferred strong antimicrobial activity against selective bacterial and fungal phytopathogens. The antimicrobial activity of the AgNPs was observed against Erwinia sp., Pseudomonas syringe, Bacillus megaterium, Fusarium graminearum, F. avenaceum and F. culmorum. The effective concentration against bacterial pathogens was found to be between 50 µg/ml (garlic/Erwinia sp.) and 130 µg/ml (onion/B. megaterium). In the case of fungal pathogens, the range was 90 µg/ml (garlic/F. avenaceum) to 110 µg/ml (onion/F. graminearum) for an effective dose.

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Abbreviations

AgNPs:

Silver nanoparticles

FTIR:

Fourier transform infrared

MAS:

Marker-assisted selection

TEM:

Transmission electron microscope

XRD:

X-ray diffraction

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Acknowledgements

The authors gratefully acknowledge the Department of Chemistry, Lovely Professional University, for providing all the necessary facilities to carry out the research.

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Authors and Affiliations

  1. School of Biotechnology and Biosciences, Lovely Professional University, Phagwara, Punjab, 144411, India

    Nitu Gautam, Neha Salaria, Kajal Thakur, Sarvjeet Kukreja & Umesh Goutam

  2. Central Instumentation Laboratory, Central University of Punjab, Bathinda, Punjab, 151001, India

    Neha Yadav

  3. Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar, Haryana, 125001, India

    Rakesh Yadav

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  1. Nitu Gautam
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  2. Neha Salaria
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  3. Kajal Thakur
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Correspondence to Umesh Goutam.

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Significance Statement

Higher crop losses due to phytopathogens have been a major problem in agriculture. Due to the limitations of conventional methods for disease control, there is a need for alternate approaches. The urge for effective novel approach has shifted the focus towards nanobiotechnology. Chemical synthesis of nanoparticles is considered as non-eco-friendly and expensive. Therefore, biosynthesis of nanoparticles through plants has appeared as a solution of above mentioned problem. So, the present study was focused on synthesis and use of metal nanoparticles against phystopathogens, which could play promising role in crop protection and disease management.

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Gautam, N., Salaria, N., Thakur, K. et al. Green Silver Nanoparticles for Phytopathogen Control. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 90, 439–446 (2020). https://doi.org/10.1007/s40011-019-01115-8

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  • DOI: https://doi.org/10.1007/s40011-019-01115-8

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