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In Vitro Selection of Cell Lines in Punica granatum L. (Daru) Against Bacterial Blight

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Abstract

Disease resistant cell lines of wild pomegranate were selected against bacterial blight caused by Xanthomonas axonopodis pv. punicae. Callus from cotyledon explants was exposed to purified toxic culture filtrate produced by bacteria. Initiation, maintenance of callus as well as selection process was carried out on MS medium supplemented with 2.0 mg/l BAP and 4.0 mg/l NAA. Resistant lines were selected at 40 % level of culture filtrate after two cycles of selection. Microshoots from selected calli were regenerated on medium containing 2.0 mg/l BAP, 1.5 mg/l Kinetin and 3.0 mg/l GA3. Fresh weight of callus increased progressively up to third subculture passage while shoot bud induction from selected calli was observed only after third subculturing. The selected microshoots were rooted on half strength MS medium containing 400 mg/l activated charcoal medium. After in vitro testing of shoots regenerated from selected calli four resistant plantlets were obtained.

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Acknowledgments

This study was conducted in the Laboratory of Department of Biotechnology and the research was financially fully supported by Dr. Yashwant Singh Parmar University of Horticulture and Forestry.

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Authors and Affiliations

  1. Department of Biotechnology, Dr. Y.S. Parmar University of Horticulture and Forestry, Solan, HP, 173 230, India

    Garima Kumari, Kamlesh Kanwar, Madhvi Soni & Deepika Sharma

Authors
  1. Garima Kumari
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  2. Kamlesh Kanwar
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  3. Madhvi Soni
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  4. Deepika Sharma
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Correspondence to Madhvi Soni.

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Kumari, G., Kanwar, K., Soni, M. et al. In Vitro Selection of Cell Lines in Punica granatum L. (Daru) Against Bacterial Blight. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 87, 591–597 (2017). https://doi.org/10.1007/s40011-015-0637-3

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  • DOI: https://doi.org/10.1007/s40011-015-0637-3

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