Skip to main content
SpringerLink
Log in

Molecular and Cultural Characterization of Alternaria brassicae Infecting Cauliflower in Uttar Pradesh, India

  • Research Article
  • Published:
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

The Alternaria blight or leaf blight (Alternaria brassicae) causes severe damage to cauliflower at curd formation and seed setting stage. Roving survey revealed that the disease incidence on cauliflower in different farmer’s fields ranged from 10 to 40 % with an average incidence of 20 % in Uttar Pradesh. Twenty-three (23) isolates of A. brassicae were collected from different cultivars in Uttar Pradesh and characterized for cultural, morphological, pathogenic and molecular variations. Based on the pathogenicity, isolates of A. brassicae were rated as virulent or less virulent based on the percentage disease incidence on cv. Hajipur local. Most of the isolates showed smooth mycelial growth with circular, irregular margin and without concentric zonation. The colony colour is white, dark brown to light brown and pinkish in white. Significant morphological variations in conidial length, conidial width, and number of horizontal septa were observed in all the isolates. The maximum length of conidia ranged from 150 to 122 μm with 8 to 9 transverse and 2 vertical septation. Further the genetic diversity of isolates based on RAPD-PCR using sixteen random primers were produced on clarity, repeatability and the number of polymorphic bands in all the isolates. Cluster analysis of DNA fragments was performed using NTSYSpc V2.2 based on UPGMA method and Jacard coefficient. Based on the analysis the isolates represented four major groups with 75 % similarity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Meena PD, Rani A, Meena R, Sharma P, Gupta R, Chowdappa P (2012) Aggressiveness, diversity and distribution of Alternaria brassicae isolates infecting oilseed Brassica in India. Afr J Microbiol 6:5249–5525

    Google Scholar 

  2. Pandey KK, Pandey PK, and Satpathy S (2002) Integrated management of disease and insects of tomato, chilli and cole crops. Technical bulletin-9, p 7

  3. Pandey KK, Pandey PK, Kalloo G, Banerjee MK (2003) Resistance to early blight of tomato with respect to various parameters of disease epidemics. J Gen Plant Pathol 69(6):364–371

    Article  Google Scholar 

  4. Simmons EG (1967) Typification of Alternaria, Stemphylium, and Ulocladium. Mycologia 59:67–92

    Article  CAS  PubMed  Google Scholar 

  5. Meena PD, Chattopadhyay C, Kumar VR, Meena RL, Rana US (2005) Spore behaviour in atmosphere and trends in variability of Alternaria brassicae population in India. J Mycol Plant Pathol 35:511

    Google Scholar 

  6. Raeder U, Broda P (1985) Rapid preparation of DNA from filamentous fungi. Lett Appl Microbiol 1:17–20

    Article  CAS  Google Scholar 

  7. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 18:6531–6535

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Rolhf FJ (1990) NTSYSPc, Numerical taxonomy and multivariant analysis system. Version 2.02. Appl. Biostatics New York

  9. Sneath PA, Sokal RR (1973) Numerical Taxonomy. Freeman, San Francisco, p 573

    Google Scholar 

  10. Sharma TR, Singh BM (1994) Induction of sporulation in a non-sporulating isolate of Alternaria brassicae (Berk.) Sacc Plant Dis Res 9(1):84–86

  11. Sharma M, Swati D, Bhati DSP, Chowdappa R, Selvamani P (2013) Morphological, cultural, pathogenic and molecular studies of Alternaria brassicae infecting cauliflower and mustard in India. Afr J Microbiol Res 7(26):3351–3363

    Google Scholar 

  12. Zhang J, Zhang T, Wang Jin Zhang JZ, Zhang TY, Wang J (1997) Classification of Alternaria species on their cultural characters. J Zhejiang Agri Univ 23(5):511–514

    Google Scholar 

  13. Swati D, Sharma P (2012) Host age as predisposing factor for incidence of black leaf spot of cauliflower caused by Alternaria brassicae and Alternaria brassicicola. Indian Phytopathol 65(1):71–75

    Google Scholar 

  14. Sharma TR, Tewari JP (1995) Detection of genetic variation in Alternaria brassicae by RAPD fingerprints. J Plant Biochem Biotechnol 4:105–107

    Article  CAS  Google Scholar 

  15. Sharma TR, Tewari JP (1998) RAPD analysis of three Alternaria species pathogenic to crucifers. Mycol Res 102:807–814

    Article  CAS  Google Scholar 

  16. Pramila GP, Mohd T, Taj G, Mal R, Kumar A (2014) Morphological, cultural, pathogenic and molecular variability amongst Indian mustard isolates of Alternaria brassicae in Uttarakhand. Afr J Biotechnol 13(3):441–448

    Article  Google Scholar 

Download references

Acknowledgments

The authors duly acknowledge the enthusiasm and support rendered by Director, IIVR during the course of the study. There is no conflict of interest regarding the conducted research work.

Author information

Authors and Affiliations

  1. Indian Institute of Vegetable Research, Varanasi, India

    Sujoy Saha, Ruchi Garg, V. Venkataravanappa, P. K. Mishra, A. B. Rai & R. P. Singh

Authors
  1. Sujoy Saha
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ruchi Garg
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. Venkataravanappa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. K. Mishra
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. B. Rai
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. R. P. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ruchi Garg.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Saha, S., Garg, R., Venkataravanappa, V. et al. Molecular and Cultural Characterization of Alternaria brassicae Infecting Cauliflower in Uttar Pradesh, India. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 86, 485–495 (2016). https://doi.org/10.1007/s40011-014-0472-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40011-014-0472-y

Keywords

Search

Navigation