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In vitro Study and Micropropagation of Ethnomedicinally Important Bryophyte: Plagiochasma appendiculatum Lehm. & Lindenb.

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Abstract

Well differentiated thalli of an ethnomedicinally potential liverwort Plagiochasma appendiculatum Lehm. & Lindenb. have been raised and multiplied in vitro in order to facilitate the availability of the living material in pure population for other experimental studies and maintenance of its germplasm. The ex plants (spores) inoculated into half strength Knop’s macronutrients and Nitsch trace elements along with 10 ppm freshly prepared ferric citrate, produced dichotomously branched thalli, while the same when supplemented with 1 % sucrose, produced a cluster of many well developed thalli within 12 weeks of culture. Multiplication of thalli was readily achieved by transferring of the apical portion of cultured thalli or innovations produced from cultured plants on fresh basal medium with above constituents. An enhanced growth has been observed in continuous light of 4,000–5,500 lux at a temperature of 21 ± 2 °C. In Murashige and Skoog medium explants showed improper growth, led to the formation of twisted and deformed thalli, while Gamborg B-5 medium produced callus like pulpy mass of undifferentiated tissue. Thalli developed in culture were acclimatized and transferred to soil for their further propagation where gametangial induction occurred at low temperature (16–18 °C) simultaneously with alternate light and dark condition of 10 and 14 h respectively.

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Acknowledgments

The authors are grateful to the Director, C.S.I.R.—National Botanical Research Institute, Lucknow for encouragement and providing facilities. Thanks are due to the Ministry of Environment & Forests, Govt. of India, New Delhi for providing financial assistance.

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  1. Bryology Laboratory, CSIR-National Botanical Research Institute, Lucknow, 226 001, India

    Vishal Awasthi, Virendra Nath & A. K. Asthana

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  1. Vishal Awasthi
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  2. Virendra Nath
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  3. A. K. Asthana
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Correspondence to A. K. Asthana.

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Awasthi, V., Nath, V. & Asthana, A.K. In vitro Study and Micropropagation of Ethnomedicinally Important Bryophyte: Plagiochasma appendiculatum Lehm. & Lindenb.. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 82, 405–412 (2012). https://doi.org/10.1007/s40011-012-0051-z

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