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Misdiagnosis of tuberculosis associated with some species of nontuberculous mycobacteria by GeneXpert MTB/RIF assay

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Abstract

Out of 12 nontuberculous mycobacteria (NTM) species, 5 were identified as Mycobacterium tuberculosis (MTB) by GeneXpert at a bacterial load of 106. Notably, two species, including M. abscessus and M. smegmatis, were flagged as RIF-resistant MTB due to the high C(t) value of Probe E. In conclusion, our data have demonstrated that the misdiagnosis of MTB by GeneXpert assay is observed in five NTM species at a high bacterial load.

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References

  1. Theron G, Peter J, Calligaro G, Meldau R, Hanrahan C, Khalfey H, et al. Determinants of PCR performance (Xpert MTB/RIF), including bacterial load and inhibition, for TB diagnosis using specimens from different body compartments. Sci Rep. 2014;4:5658.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Boehme CC, Nabeta P, Hillemann D, Nicol MP, Shenai S, Krapp F, et al. Rapid molecular detection of tuberculosis and rifampin resistance. N Engl J Med. 2010;363:1005–15.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Moure R, Munoz L, Torres M, Santin M, Martin R, Alcaide F. Rapid detection of Mycobacterium tuberculosis complex and rifampin resistance in smear-negative clinical samples by use of an integrated real-time PCR method. J Clin Microbiol. 2011;49:1137–9.

    Article  PubMed  PubMed Central  Google Scholar 

  4. Drobniewski F, Nikolayevskyy V, Balabanova Y, Bang D, Papaventsis D. Diagnosis of tuberculosis and drug resistance: what can new tools bring us? Int J Tuberc Lung Dis. 2012;16:860–70.

    Article  CAS  PubMed  Google Scholar 

  5. World Health Organization. Xpert MTB/RIF assay for the diagnosis of pulmonary and extrapulmonary TB in adults and children: policy update. Geneva: World Health Organization; 2013.

    Google Scholar 

  6. Pang Y, Lu J, Wang Y, Song Y, Wang S, Zhao Y. Study of the rifampin monoresistance mechanism in Mycobacterium tuberculosis. Antimicrob Agents Chemother. 2013;57:893–900.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Helb D, Jones M, Story E, Boehme C, Wallace E, Ho K, et al. Rapid detection of Mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology. J Clin Microbiol. 2010;48:229–37.

    Article  CAS  PubMed  Google Scholar 

  8. Hillemann D, Rusch-Gerdes S, Boehme C, Richter E. Rapid molecular detection of extrapulmonary tuberculosis by the automated GeneXpert MTB/RIF system. J Clin Microbiol. 2011;49:1202–5.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Zhang Z, Pang Y, Wang Y, Cohen C, Zhao Y, Liu C. Differences in risk factors and drug susceptibility between Mycobacterium avium and Mycobacterium intracellulare lung diseases in China. Int J Antimicrob Agents. 2015;45:491–5.

    Article  CAS  PubMed  Google Scholar 

  10. Zhang Z, Wang Y, Pang Y, Liu C. Comparison of different drug susceptibility test methods to detect rifampin heteroresistance in Mycobacterium tuberculosis. Antimicrob Agents Chemother. 2014;58:5632–5.

    Article  PubMed  PubMed Central  Google Scholar 

  11. Lee H, Bang HE, Bai GH, Cho SN. Novel polymorphic region of the rpoB gene containing Mycobacterium species-specific sequences and its use in identification of mycobacteria. J Clin Microbiol. 2003;41:2213–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Mougari F, Guglielmetti L, Raskine L, Sermet-Gaudelus I, Veziris N, Cambau E. Infections caused by Mycobacterium abscessus: epidemiology, diagnostic tools and treatment. Expert Rev Anti Infect Ther. 2016;14:1139–54.

    Article  CAS  PubMed  Google Scholar 

  13. Tiberi S, De Lorenzo S, Centis R, Viggiani P, D’Ambrosio L, Migliori GB. Bedaquiline in MDR/XDR-TB cases: first experience on compassionate use. Eur Respir J. 2014;43:289–92.

    Article  PubMed  Google Scholar 

  14. World Health Organization. Toman’s Tuberculosis: Case detection, treatment and monitoring-questions and answers. 2004; Geneva: World Health Organization.

  15. Theron G, Pinto L, Peter J, Mishra HK, van Zyl-Smit R, Sharma SK, et al. The use of an automated quantitative polymerase chain reaction (Xpert MTB/RIF) to predict the sputum smear status of tuberculosis patients. Clin Infect Dis. 2012;54:384–8.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was supported by the National Key Research Program of China (2014ZX10003002) and the National Natural Science Foundation of China (81401739). We would like to thank members of the National Tuberculosis Reference Laboratory at the Chinese Center for Disease Control and Prevention for their technical assistance.

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Author notes

  1. Yu Pang and Jie Lu contributed equally to this study.

Authors and Affiliations

  1. National Tuberculosis Clinical Laboratory, Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, No. 97 Machang, Tongzhou District, Beijing, 101149, People’s Republic of China

    Yu Pang

  2. Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, Beijing, People’s Republic of China

    Jie Lu

  3. State Key Laboratory of Respiratory Diseases, Department of Clinical Laboratory, Guangzhou Chest Hospital, Guangzhou, People’s Republic of China

    Biyi Su

  4. National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155, Chang Bai Road, Changping District, Beijing, 102206, People’s Republic of China

    Huiwen Zheng & Yanlin Zhao

Authors
  1. Yu Pang
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  2. Jie Lu
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  3. Biyi Su
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  4. Huiwen Zheng
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  5. Yanlin Zhao
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Corresponding authors

Correspondence to Yu Pang or Yanlin Zhao.

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Funding

This work was supported by the Major State Basic Research Development Program of China (2014CB744403) and the National Natural Science Foundation of China (81401739).

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

This study was approved by the Ethic Committee of Chinese Center for Disease Control and Prevention.

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Pang, Y., Lu, J., Su, B. et al. Misdiagnosis of tuberculosis associated with some species of nontuberculous mycobacteria by GeneXpert MTB/RIF assay. Infection 45, 677–681 (2017). https://doi.org/10.1007/s15010-017-1044-x

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  • DOI: https://doi.org/10.1007/s15010-017-1044-x

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