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Polydeoxyribonucleotide and Polynucleotide Improve Tendon Healing and Decrease Fatty Degeneration in a Rat Cuff Repair Model

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Tissue Engineering and Regenerative Medicine Aims and scope

A Correction to this article was published on 08 July 2023

This article has been updated

Abstract

Background:

After surgical repair of chronic rotator cuff tears, healing of the repaired tendons often fails and is accompanied by high-level fatty degeneration. Our purpose was to explore the effects of polydeoxyribonucleotide (PDRN) and polynucleotide (PN) on tendon healing and the reversal of fatty degeneration in a chronic rotator cuff tear model using a rat infraspinatus.

Methods:

Sixty rats were randomly assigned to the following three groups (20 rats per group: 12 for histological evaluation and 8 for mechanical testing): saline + repair (SR), PDRN + repair (PR), and PN + repair (PNR). The right shoulder was used for experimental intervention, and the left served as a control. Four weeks after detaching the infraspinatus, the torn tendon was repaired. Saline, PDRN, and PN were applied to the repair sites. Histological evaluation was performed 3 and 6 weeks after repair and biomechanical analysis was performed at 6 weeks.

Results:

Three weeks after repair, the PR and PNR groups had more CD168-stained cells than the SR group. The PR group showed a larger cross-sectional area (CSA) of muscle fibers than the SR and PNR groups. Six weeks after repair, the PR and PNR groups showed more adipose cells, less CD68-stained cells, and more parallel tendon collagen fibers than the SR group. The PR group had more CD 68-stained cells than the PNR group. The PR group showed a larger CSA than the SR group. The mean load-to-failure values of the PR and PNR groups were higher than that of the SR group, although these differences were not significant.

CONCLUSION:

PDRN and PN may improve tendon healing and decrease fatty degeneration after cuff repair.

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Acknowledgements

We thank Jun Sub Jung, a research agent for valuable advices. Jung-Taek Hwang have received the National Research Foundation of Korea (NRF) Grant. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT (NRF-2016R1C1B2007014).

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Author notes

  1. Jung-Taek Hwang and Sang-Soo Lee are co-first authors.

Authors and Affiliations

  1. Department of Orthopedic Surgery, Chuncheon Sacred Heart Hospital, Hallym University Medical College, 77, Sakju-ro, Chuncheon-si, Gangwon-do, 24253, Republic of Korea

    Jung-Taek Hwang, Sang-Soo Lee, Binod Sherchan & Jiss Joseph Panakkal

  2. Department of Pathology, Chuncheon Sacred Heart Hospital, Hallym University Medical College, Chuncheon-si, Gangwon-do, Republic of Korea

    Sang Hak Han

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Correspondence to Jung-Taek Hwang or Sang Hak Han.

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Ethical statement

This animal study was conducted in accordance with guidelines and approval of the Institutional Animal Care and Use Committees (IACUC) of Hallym University (Hallym-2014–22). The present study had been performed in accordance with the ethical standards in the 1964 Declaration of Helsinki. The present study had been carried out in accordance with relevant regulations of the US Health Insurance Portability and Accountability Act (HIPAA). Details that might disclose the identity of the subjects under study should be omitted. This study was presented as a podium in 2016 AAOS and as an e-poster in 2019 ISAKOS.

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Rat muscle fixation device (KR Design Registration 30–0854878).

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Hwang, JT., Lee, SS., Han, S.H. et al. Polydeoxyribonucleotide and Polynucleotide Improve Tendon Healing and Decrease Fatty Degeneration in a Rat Cuff Repair Model. Tissue Eng Regen Med 18, 1009–1020 (2021). https://doi.org/10.1007/s13770-021-00378-5

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