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Inhibitory Effect of Topical Cartilage Acellular Matrix Suspension Treatment on Neovascularization in a Rabbit Corneal Model

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

The extracellular matrix (ECM) of articular cartilage has an inhibitory effect on vascularization, yet clinical utilization has been technically challenging. In this study, we aimed to fabricate a biologically functional ECM powder suspension from porcine articular cartilage that inhibits neovascularization (NV).

Methods:

The digested-cartilage acellular matrix (dg-CAM) was prepared by sequential processes of decellularization, enzymatic digestion and pulverization. Physicochemical properties of dg-CAM were compared with that of native cartilage tissue (NCT). Cellular interactions between human umbilical vein endothelial cells (HUVECs) and dg-CAM was evaluated with proliferation, migration and tube formation assays compared with that of type I collagen (COL) and bevacizumab, an anti-angiogenic drug. We then investigated the therapeutic potential of topical administration of dg-CAM suspension on the experimentally induced rabbit corneal NV model.

Results:

The dg-CAM released a significantly larger amount of soluble proteins than that of the NCT and showed an improved hydrophilic and dispersion properties. In contrast, the dg-CAM contained a large amount of collagen, glycosaminoglycans and anti-angiogenic molecules as much as the NCT. The inhibitory effect on NV of the dg-CAM was more prominent than that of COL and even comparable to that of bevacizumab in inhibiting the HUVECs. The therapeutic potential of the dg-CAM was comparable to that of bevacizumab in the rabbit corneal NV model by efficiently inhibiting neovessel formation of the injured cornea.

Conclusion:

The current study developed a dg-CAM having anti-angiogenic properties, together with water-dispersible properties suitable for topical or minimally invasive application for prevention of vessel invasion.

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Acknowledgement

This study was supported by a grant from the Korea Health Technology R&D Project (HI14C0744 and HI17C2191) through the Korea Health Industry Development Institute funded by the Ministry of Health & Welfare.

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Authors and Affiliations

  1. Department of Molecular Science and Technology, Ajou University, San 5, Wonchon-dong, Youngtong-gu, Suwon, 16499, Republic of Korea

    Hee-Woong Yun & Byoung-Hyun Min

  2. Cell Therapy Center, Ajou University School of Medicine, San 5, Wonchon-dong, Youngtong-gu, Suwon, 16499, Republic of Korea

    Hee-Woong Yun & Byoung-Hyun Min

  3. Department of Biomedical Sciences, Inha University College of Medicine, 100, Inha-ro, Michuhol-gu, Incheon, 22212, Republic of Korea

    Byung Hyune Choi

  4. Department of Orthopedic Surgery, Ajou University School of Medicine, San 5, Wonchon-dong, Youngtong-gu, Suwon, 16499, Republic of Korea

    Do Young Park & Byoung-Hyun Min

  5. Department of Orthopedic Surgery, Yanbian University Medical School, 977 Gongyuan Rd, Yanji, Yanbian, China

    Long Hao Jin

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  1. Hee-Woong Yun
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Correspondence to Byoung-Hyun Min.

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The animal study was approved by the Laboratory Animal Research Center of Ajou University Medical Center (No, 2013-0014) and performed according to the regulations of the ARVO statement for Ophthalmic and Visual research.

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Yun, HW., Choi, B.H., Park, D.Y. et al. Inhibitory Effect of Topical Cartilage Acellular Matrix Suspension Treatment on Neovascularization in a Rabbit Corneal Model. Tissue Eng Regen Med 17, 625–640 (2020). https://doi.org/10.1007/s13770-020-00275-3

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  • DOI: https://doi.org/10.1007/s13770-020-00275-3

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