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The Effect of Fibronectin-Immobilized Microgrooved Titanium Substrata on Cell Proliferation and Expression of Genes and Proteins in Human Gingival Fibroblasts

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

We aimed to determine the effect of fibronectin (FN)-immobilized microgrooved titanium (Ti) on human gingival fibroblast proliferation, gene expression and protein expression.

Methods:

Photolithography was used to fabricate the microgrooved Ti, and amine funtionalization (silanization) was used for FN immobilization on titanium surfaces. Cell proliferation, gene expression and protein expression were analyzed, followed by multiple regression analysis for determining the influential factors on cell proliferation.

Results:

FN-immobilized microgrooved Ti significantly enhanced the fibroblast proliferation in various timelines of culture, among which a burst of fivefold increase is induced at 96 h of culture compared to that on the control smooth Ti. We suggest a presence of the synergistic promotion effect of microgrooves and FN immobilization on fibroblast proliferation. Through a series of analyses on the expression of various genes and proteins involved in cell adhesion and proliferation, cyclin-dependent kinase 6, cyclin D1, integrin α5, oncogene c-Src, osteonectin, paxillin and talin-2 were determined as influential factors on promoting fibroblast proliferation induced by FN-immobilized microgrooved Ti.

Conclusion:

FN-immobilized microgrooved Ti can act as an effective surface for enhancing fibroblast proliferation, and can be used for promoting soft tissue response on the connective tissue attachment zone of biomaterial surfaces.

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Acknowledgements

This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, Republic of Korea (Grant No. HI16C1838).

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Pathology and Institute of Oral Biology, School of Dentistry, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea

    Eun-Cheol Kim

  2. Doctorplant Dental Clinic, 885 Gyeongin-ro, Yeongdeungpo-gu, Seoul, 07303, Republic of Korea

    Do Yun Lee

  3. Energy and Environmental Division, Korea Institute of Ceramic Engineering and Technology, 101 Soho-ro, Jinju-si, Gyeongsangnam-do, 52851, Republic of Korea

    Myung-Hyun Lee

  4. Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea

    Hong Jae Lee & Sang Cheon Lee

  5. Core Research Laboratory, Clinical Research Institute, Kyung Hee University Hospital at Gangdong, 892 Dongnam-ro, Gangdong-gu, Seoul, 05278, Republic of Korea

    Kyung-Hee Kim

  6. Department of Biomaterials and Prosthodontics, Kyung Hee University Hospital at Gangdong, Institute of Oral Biology, School of Dentistry, Kyung Hee University, 892 Dongnam-ro, Gangdong-gu, Seoul, 05278, Republic of Korea

    Richard Leesungbok, Su-Jin Ahn & Suk Won Lee

  7. Department of Prosthodontics, Dankook University Sejong Dental Hospital, 87 Doum 8-ro, Sejong, 30107, Republic of Korea

    Su-Jung Park

  8. Department of Dentistry, National Health Insurance Medical Center Ilsan Hospital, 100 Ilsan-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10444, Republic of Korea

    Joon-Ho Yoon

  9. Department of Oral and Maxillofacial Surgery, Kyung Hee University Hospital at Gangdong, Institute of Oral Biology, School of Dentistry, Kyung Hee University, 892 Dongnam-ro, Gangdong-gu, Seoul, 05278, Republic of Korea

    Yu-Jin Jee

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  1. Eun-Cheol Kim
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Correspondence to Sang Cheon Lee or Suk Won Lee.

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The authors declare that they have no conflict of interest.

Ethical statement

This study was approved by the Institutional Review Board of Kyung Hee University Hospital at Gangdong (KHNMC MD IRB 2010-011).

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Kim, EC., Lee, D.Y., Lee, MH. et al. The Effect of Fibronectin-Immobilized Microgrooved Titanium Substrata on Cell Proliferation and Expression of Genes and Proteins in Human Gingival Fibroblasts. Tissue Eng Regen Med 15, 615–627 (2018). https://doi.org/10.1007/s13770-018-0153-7

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  • DOI: https://doi.org/10.1007/s13770-018-0153-7

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