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Comparative study of recombinant human bone morphogenetic protein-2 carriers in rat subcutaneous tissues: Pilot study

  • Original Article
  • Cell Biology
  • Published:
Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

The purpose of this study was to evaluate and compare the potential of using various biomaterials as a carrier of rhBMP-2 in the subcutaneous layer of rats. 5 subcutaneous defects were made in each of the dorsal surfaces of 10 rats. Then, the rats were divided into the following groups: Control group: sham-surgery control, Experimental group 1: rhBMP-2/Bio-Oss®, Experimental group 2: rhBMP-2/Collatape®, Experimental group 3: rhBMP-2/nCS. Histological and histometrical analysis were performed after the rats were sacrificed at 2 and 4 weeks. Regardless of the type of carrier, the groups treated with rhBMP-2 showed significantly greater bone formation when compared to the control group. The amount of new bone formation was greater at 4 week than 2 week. Total bone formation (%) at 4 week was the greatest in rhBMP/Bio-Oss® (26.76±10.13) group, followed by rhBMP/Collatape® (20.38±5.27) and rhBMP/nCS (4.34±2.58). Within the limitation of this study, the results showed that Bio-Oss®, Collatape®, nanocalcium sulfate all showed new bone formation when used as a carrier of rhBMP-2 in the subcutaneous layer of rats, indicating potential use as carriers for rhBMP-2.

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Authors and Affiliations

  1. Department of Prosthodontics, Oral Science Research Center, Yonsei University College of Dentistry, 50 Yonseiro, Seodaemun-gu, Seoul, 120-752, Korea

    Sunkyu Lee, Hyunmin Choi, June-Sung Shim, Moon-Kyu Chung & Young-Bum Park

  2. Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Korea

    June-Sung Shim, Moon-Kyu Chung & Young-Bum Park

Authors
  1. Sunkyu Lee
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  2. Hyunmin Choi
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  3. June-Sung Shim
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  4. Moon-Kyu Chung
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  5. Young-Bum Park
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Corresponding authors

Correspondence to Moon-Kyu Chung or Young-Bum Park.

Additional information

Sunkyu Lee and Hyunmin Choi equally contributed to the works described in this manuscript.

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Lee, S., Choi, H., Shim, JS. et al. Comparative study of recombinant human bone morphogenetic protein-2 carriers in rat subcutaneous tissues: Pilot study. Tissue Eng Regen Med 12 (Suppl 2), 138–146 (2015). https://doi.org/10.1007/s13770-015-0437-0

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  • DOI: https://doi.org/10.1007/s13770-015-0437-0

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