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Supplementation of growth differentiation factor-5 increases proliferation and size of chondrogenic pellets of human umbilical cord-derived perivascular stem cells

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

In recent years, perivascular stem cells (PVCs) have gained increasing attention as a promising source for regenerative medicine due to their greater differentiation potential compared to multipotent mesenchymal stem cells (MSCs). It has been reported that growth differentiation factor-5 (GDF-5) is involved in regulating proliferation and chondrogenic differentiation of MSCs. In this study, we investigated the effect of GDF-5 on the proliferation and chondrogenic differentiation of PVCs isolated from human umbilical cords. The supplementation of PVC cultures with GDF-5 (100 ng/mL) significantly enhanced their proliferative rate and augmented the size of pellets in micromass pellet cultures for chondrogenic induction. Although similar expression levels of chondrogenic-related genes were observed in chondrogenic pellets treated with GDF-5 compared to the pellets without GDF-5 treatment, these results indicate that supplementation of GDF-5 is able to acquire more chondrocytes when starting with equal amount of PVCs. Our study suggests that GDF-5 is an effective agent for the enhancement of PVC proliferation, thereby achieving a higher number of chondrocytes that are applicable in therapeutic doses for cartilage regeneration.

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Authors and Affiliations

  1. Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon, Korea

    Borim An, Hye-Ryeon Heo, Sungeun Lee, Jeong-Ah Park & Seok-Ho Hong

  2. Graduate School of Biomedical Science and Engineering, Department of Biomedical Science, Hanyang University, Seoul, Korea

    Kye-Seong Kim

  3. Department of Family Medicine, Kangwon National University Hospital, School of Medicine, Kangwon National University, Chuncheon, Korea

    Jeonghee Yang

  4. Stem Cell Institute, Kangwon National University, Chuncheon, Korea

    Seok-Ho Hong

  5. Department of Family Medicine, School of Medicine, Kangwon National University, 1 Gangwondaehak-gil, Chuncheon, 200-701, Korea

    Jeonghee Yang

  6. Department of Internal Medicine, School of Medicine, Kangwon National University, 1 Gangwondaehak-gil, Chuncheon, 200-701, Korea

    Seok-Ho Hong

Authors
  1. Borim An
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  2. Hye-Ryeon Heo
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  4. Jeong-Ah Park
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  5. Kye-Seong Kim
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  6. Jeonghee Yang
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  7. Seok-Ho Hong
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Corresponding authors

Correspondence to Jeonghee Yang or Seok-Ho Hong.

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These authors equally contributed to this study.

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An, B., Heo, HR., Lee, S. et al. Supplementation of growth differentiation factor-5 increases proliferation and size of chondrogenic pellets of human umbilical cord-derived perivascular stem cells. Tissue Eng Regen Med 12, 181–187 (2015). https://doi.org/10.1007/s13770-015-0113-4

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  • DOI: https://doi.org/10.1007/s13770-015-0113-4

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