Abstract
Synthetic degradable polymer scaffolds have often shown poor cell affinity and bone bioactivity. Bioactive inorganic nanoadditives with the composition of calcium silicate in amorphous phase (CSN) were incorporated within poly (lactic acid) (PLA) to develop nanocomposite bone scaffolds. Nanocomposite solutions containing PLA with 10% CSN were porous-structured by a salt-impregnation / leaching method. The nanocomposite scaffold showed higher dynamical mechanical modulus values than the pure PLA equivalent, indicating a proper usage for hard tissue scaffolds. Rat bone marrow stromal cells (rBMSCs) were cultured on the scaffolds and the cell adhesion, proliferation and osteogenic differentiation at gene and protein levels were addressed. Cells adhered better on the nanocomposite scaffold with respect to PLA equivalent, and then proliferated more during the culture periods of up to 7 days. The expression of osteogenic genes, including collagen type I, osteopontin, osteocalcin and alkaline phosphatase, were significantly stimulated on the nanocomposite scaffold, as confirmed by a real time polymerase chain reaction. Furthermore, Western blot analysis indicated higher expression of osteopontin protein of cells by the nanocomposite scaffold. Results demonstrated the bioactive nanocomposite scaffolds provided appropriate matrix conditions for rBMSCs to populate on and to undergo osteogenic processes in vitro.
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PK Yarlagadda, M Chandrasekharan, JY Shyan, Recent advances and current developments in tissue scaffolding, Biomed Mater Eng, 15, 159 (2005).
RA Perez, HW Kim, MP Ginebra, Polymeric additives to enhance the functional properties of calcium phosphate cements, J Tissue Eng, 3, 2041731412439555 (2012).
KA Athanasiou, C Zhu, DR Lanctot, et al., Fundamentals of biomechanics in tissue engineering of bone, Tissue Eng, 6, 361 (2000).
NG Sahoo, YZ Pan, L Li, et al., Nanocomposites for bone tissue regeneration, Nanomedicine (Lond), 8, 639 (2013).
C Du, FZ Cui, XD Zhu, et al., Three-dimensional nano-HAp/collagen matrix loading with osteogenic cells in organ culture, J Biomed Mater Res, 44, 407 (1999).
RZ Wang, FZ Cui, HB Lu, et al., Synthesis of nanophase hydroxyapatite-collagen composite, J Mater Sci Lett, 14, 490 (1995)
JH Song, HE Kim, HW Kim, Collagen-apatite nanocomposite membranes for guided bone regeneration, J Biomed Mater Res B Appl Biomater, 83, 248 (2007).
HW Kim, HJ Gu, HH Lee, Microspheres of collagen-apatite nanocomposites with osteogenic potential for tissue engineering, Tissue Eng, 13, 965 (2007).
JH Song, HE Kim, HW Kim, Electrospun fibrous web of collagen-apatite precipitated nanocomposite for bone regeneration, J Mater Sci Mater Med, 19, 2925 (2008).
Shin SH, Purevdorj O, Oscar C, Planell JA, Kim HW. A short review: recent advances in electrospinning for bone tissue regeneration, J Tissue Eng, 3, 2041731412443530 (2012).
HW Kim, Biomedical nanocomposites of hydroxyapatite/polycaprolactone obtained by surfactant mediation, J Biomed Mater Res A, 83, 169 (2007).
SH Jegal, JH Park, JH Kim, et al., Functional composite nanofibers of poly(lactide-co-caprolactone) containing gelatinapatite bone mimetic precipitate for bone regeneration, Acta Biomater, 7, 1609 (2011).
JE Won, A El-Fiqi, SH Jegal, et al., Gelatin-apatite bone mimetic co-precipitates incorporated within biopolymer matrix to improve mechanical and biological properties useful for hard tissue repair, J Biomater Appl, 28, 1213 (2014).
HW Kim, HH Lee, JC Knowles, Electrospinning biomedical nanocomposite fibers of hydroxyapatite/poly(lactic acid) for bone regeneration, J Biomed Mater Res A, 79, 643 (2006).
RK Singh, KD Patel, JH Lee, et al., Potential of magnetic nanofiber scaffolds with mechanical and biological properties applicable for bone regeneration, PLoS One, 9, e91584 (2014).
JH Jo, EJ Lee, DS Shin, et al., In vitro/in vivo biocompatibility and mechanical properties of bioactive glass nanofiber and poly (epsilon-caprolactone) composite materials, J Biomed Mater Res B Appl Biomater, 91, 213 (2009).
HH Lee, HS Yu, JH Jang JH, et al., Bioactivity improvement of poly(epsilon-caprolactone) membrane with the addition of nanofibrous bioactive glass, Acta Biomater, 4, 622 (2008).
HW Kim, JH Song, HE Kim, Bioactive glass nanofibercollagen nanocomposite as a novel bone regeneration matrix, J Biomed Mater Res A, 79, 698 (2006).
A El-Fiqi, JH Lee, EJ Lee, et al., Collagen hydrogels incorporated with surface-aminated mesoporous nanobioactive glass: Improvement of physicochemical stability and mechanical properties is effective for hard tissue engineering, Acta Biomater, 9, 9508 (2013).
A El-Fiqi, TH Kim, M Kim, et al., Capacity of mesoporous bioactive glass nanoparticles to deliver therapeutic molecules, Nanoscale, 4, 7475 (2012).
TA Owen, M Aronow, V Shalhoub, et al., Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix, J Cell Physiol, 143, 420 (1990).
A Oldberg, A Franzen, D Heinegard, Cloning and sequence analysis of rat bone sialoprotein (osteopontin) cDNA reveals an Arg-Gly-Asp cell-binding sequence, Proc Natl Acad Sci U S A, 83, 8819 (1986).
JB Lian, GS Stein, Concepts of osteoblast growth and differentiation: Basis for modulation of bone cell development and tissue formation, Crit Rev Oral Biol Med, 3, 269 (1992).
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Jin, GZ., Kim, HW. Nanocomposite bioactive polymeric scaffold promotes adhesion, proliferation and osteogenesis of rat bone marrow stromal cells. Tissue Eng Regen Med 11, 284–290 (2014). https://doi.org/10.1007/s13770-014-0033-8
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DOI: https://doi.org/10.1007/s13770-014-0033-8