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Adipose tissue-derived stem cells in the ovariectomy-induced postmenopausal osteoporosis rat model

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

This study investigated the efficacy of adult adipose tissue-derived stem cells in restoring bone using an osteoporotic rat model. Thirty-six female Wistar rats (250–300 g, 12 weeks) were randomized into three equal groups: SHAM group (sham-operated), ovariectomy-induced (OVX) group and OVX with stem cell injection group (OVX with stem). Femur extraction and blood sampling were performed at 5, 6, 7 and 8 weeks. The proximal femoral metaphysis was scanned by micro-CT and evaluated for changes in various histomorphometric parameters. β-catenin expression was determined using H&E and immunohistochemistry. Bone metabolism was assessed using measurements of C-telopeptide of collagen type I (CTX), osteocalcin, and bone alkaline phosphatase (BALP). There was a trend for an increase in the bone mineral density (BMD), bone volume/trabecular bone volume (BV/TV) and trabecular number (Tb.N) in the OVX with stem group as compared with the OVX group, and decrease in the OVX with stem group as compared with SHAM group. The trabecular thickness (Tb.Th) and trabecular separation (Tb.Sp) of the OVX group were increased compared with those values in the SHAM and OVX with stem group. BALP levels were higher in the OVX group as compared with that in the other two groups and osteocalcin levels were highest in the SHAM group and slightly increased in the OVX with stem group as compared with the OVX group. This study may help us gain an understanding of the role of MSCs in the pathophysiology and treatment of osteoporosis.

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Authors and Affiliations

  1. Department of Neurological surgery, Soonchunhyang Unversity Bucheon Hospital, College of Medicine, Bucheon, Korea

    Je Hoon Jeong

  2. Laboratory of Stem Cell Therapy, College of Medicine, Asan Medical Center, University of Ulsan, Ulsan, Korea

    Je Hoon Jeong, JuRi Park, Eun-Sun Jin, JoongKee Min, Sang Ryong Jeon & Kyoung Hyo Choi

  3. Department of internal medicine, KyungHee University, College of Medicine, Seoul, Korea

    Eun-Sun Jin

  4. Department of Neurological surgery, Asan Medical Center, University of Ulsan, College of Medicine, Ulsan, Korea

    Sang Ryong Jeon

  5. Department of Rehabilitation Medicine, Asan Medical Center, University of Ulsan, College of Medicine, Ulsan, Korea

    Kyoung Hyo Choi

  6. Department of Physical Medicine and Rehabilitation, Kosin University, College of Medicine, Busan, Korea

    Dong Kyu Kim

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  1. Je Hoon Jeong
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  2. JuRi Park
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  3. Eun-Sun Jin
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  7. Kyoung Hyo Choi
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Correspondence to Kyoung Hyo Choi.

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Jeong, J.H., Park, J., Jin, ES. et al. Adipose tissue-derived stem cells in the ovariectomy-induced postmenopausal osteoporosis rat model. Tissue Eng Regen Med 12, 28–36 (2015). https://doi.org/10.1007/s13770-014-0001-3

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  • DOI: https://doi.org/10.1007/s13770-014-0001-3

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