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Protective effect of liensinine on periodontitis through its antioxidant effect in mice

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Abstract

Liensinine is the most important functional compound in lotus seeds; its protective effect on periodontitis was determined and the mechanism of its antioxidant effect was also explained. During the experiment, we could observe that liensinine could reduce the gingival index in periodontitis-induced mice, and these effects were similar to that during the treatment of periodontitis with the metronidazole drug. After the treatment with liensinine, the serum Immunoglobulin G1 (IgG1) level was found to increase and IgG2a level was found to decrease compared to the periodontitis control mice. Liensinine and metronidazole could increase the superoxide dismutase (SOD) serum levels in the periodontitis-induced mice, and both the drugs at the same concentration (200 mg/kg) showed similar SOD activities, which was found to increase. Liensinine also increased the oxidation-related factor of catalase (CAT), glutathione peroxidase, and decreased nitric oxide, malondialdehyde, and endothelin in the serum of mice, compared to the periodontitis control mice. The serum cytokine levels of Interleukin-6, tumor necrosis factor-α, and Interferon-γ in liensinine-treated mice were lower than the control mice, but higher than the normal mice. The serum IL-4 cytokine level of liensinine-treated mice was reversed, i.e., higher than the control mice. In the periodontal tissue, the mRNA and protein expressions of inhibitor of kappa B-α, transforming growth factor beta 1, Interleukin-10, Mn-SOD, Gu/Zn-SOD, and CAT were increased by the treatment with liensinine compared to the control group mice, and nuclear factor kappa B expressions showed the opposite trend. Liensinine is a good functional compound in the treatment of periodontitis, similar to metronidazole drug. Higher concentration of liensinine had better effects.

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    Authors and Affiliations

    1. Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Chongqing Medical University, 426 Songshi North Street, Yubei District, Chongqing, 401147, People’s Republic of China

      Liang Pang, Lihua Qiu, Xiang Gao, Jiang Deng & Ying Li

    2. Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing, 401147, People’s Republic of China

      Liang Pang, Lihua Qiu, Xiang Gao, Jiang Deng & Ying Li

    3. School of Biological and Chemical Engineering, Chongqing University of Education, 9 Xuefu Main Street, Nan’an District, Chongqing, 400067, People’s Republic of China

      Kai Zhu, Xia Feng & Xin Zhao

    4. Chongqing Collaborative Innovation Center of Functional Food, Chongqing University of Education, Chongqing, 400067, People’s Republic of China

      Kai Zhu, Xia Feng & Xin Zhao

    5. School of Public Health and Management, Chongqing Medical University, Chongqing, 400016, People’s Republic of China

      Weiwei Liu

    6. Chongqing Enterprise Engineering Technology Research Center of Ba Lotus Varieties Breeding and Deep Processing, Chongqing, 400041, People’s Republic of China

      Deguang Peng & Xin Zhao

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    1. Liang Pang
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    Correspondence to Ying Li or Xin Zhao.

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    Liang Pang and Kai Zhu have contributed equally to this work.

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    Pang, L., Zhu, K., Feng, X. et al. Protective effect of liensinine on periodontitis through its antioxidant effect in mice. J Korean Soc Appl Biol Chem 58, 927–936 (2015). https://doi.org/10.1007/s13765-015-0122-y

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