Skip to main content
SpringerLink
Log in

Fluctuation of 20-hydroxyecdysone in individual organs of Achyranthes japonica during reproductive growth stage and its accumulation into seed

  • Short Communication
  • Published:
Journal of the Korean Society for Applied Biological Chemistry Submit manuscript

Abstract

To better understand 20-hydroxyecdysone (20E) fluctuation and accumulation in perennial plant, 20E concentration in individual organs of Achyranthes japonica during reproductive growth stage were analyzed by high performance liquid chromatography (HPLC). Concentrations of 20E in root and floral part were much higher than those in stem and leaf during reproductive growth stage and rapidly increased from flowering stage in August to seed-setting stage in October, and thereafter decreased at the stage of seed maturing in November. In contrast, the 20E concentrations in stem and leaf gradually decreased during reproductive growth. In the analysis of detailed fluctuation of 20E in floral part, the 20E concentration was highest in the seed at the early stage of seed development, compared to flower, peduncle, seed coat, and/or seed in other growth stages, and decreased during seed maturation. The accumulation of 20E in reproductive organs, especially seed and root, suggests that 20E has a defensive role for protection of especially newly developing organs against phytophagous insects.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Adler J and Grebenok R (1999) Occurrence, biosynthesis, and putative role of ecdysteroids in plants. Crit Rev Biochem Mol Biol 34, 253–264.

    Article  CAS  Google Scholar 

  • Arnault C and Sláma K (1986) Dietary effects of phytoecdysones in the leekmoth, Acrolpiopsis assectella Zell. (Lepidoptera: Acrolepiidae). J Chem Ecol 12, 1979–1986.

    Article  CAS  Google Scholar 

  • Bakrim A, Maria A, Sayah F, Lafont R and Takvorian N (2008) Ecdysteroids in spinach (Spinacia oleracea L.): Biosynthesis, transport and regulation of levels. Plant Physiol Biochem 46, 844–854.

    Article  CAS  Google Scholar 

  • Bergamasco E and Horn DHS (1985) Distribution and role of insect hormones in plants. In Endocrinology of Insects (vol. 1), Downer RGH, Laufer H (eds.), pp. 627–654, Alan R Liss, New York, USA.

    Google Scholar 

  • Blackford M and Dinan L (1997) The effects of ingested 20-hydroxyecdysone on the larvae of Aglais urticae, Inachis io, Cynthia cardui (Lepidoptera: Nymphalidae) and Tyria jacobaeae (Lepidoptera: Arctiidae). J Insect Physiol. 43, 315–327.

    Article  CAS  Google Scholar 

  • Boo KH, Kim-Cho S, Chae HB, Jin SB, Lee DS, Kim DW et al. (2002) Isolation of genes involved in ecdysteroids biosynthesis from Achyranthes japonica Nakai. J Korean Soc Agric Chem Biotechnol 44, 153–161.

    Google Scholar 

  • Boo KH, Lee D, Jeon GL, Ko SH, Cho SK, Kim JH et al. (2010) Distribution and biosynthesis of 20-hydroxyecdysone in plants of Achyranthes japonica Nakai. Biosci Biotechnol Biochem 74, 222631.

    Article  Google Scholar 

  • Chae HB, Boo KH, Jin SB, Lee DS, Kim DW, Cho MJ et al. (2001) Effects of light and some plant growth regulators on ecdysteroid contents in Polypodium vulgare L. and Achyranthes japonica Nakai. J Korean Soc Agric Chem Biotechnol 44, 162–166.

    CAS  Google Scholar 

  • Chi D, MingXue S and WenFu X (2002) Pesticidal character of phytoecdysteroids from Ajuga multiflora Bunge (Labiatae) on larvae of Cryptorrhynchus lapathi L. (Coleoptera: Curculionidae). Journal of Forestry Research 13, 177–182.

    Article  CAS  Google Scholar 

  • Dinan L (1992) The analysis of phytoecdysteroids in single (preflowering stage) specimens of fat hen, Chenopodium album. Phytochem Anal 3, 132–138.

    Article  CAS  Google Scholar 

  • Dinan L (1995) Distribution and levels of phytoecdysteroids within individual plants of species of the Chenopodiaceae. Eur J Entomol 92, 295–300.

    CAS  Google Scholar 

  • Dinan L, Savechenko T and Whiting P (2001) On the distribution of phytoecdysteroids in plants. Cell Mol Life Sci 58, 1121–1132.

    Article  CAS  Google Scholar 

  • Grebenok RJ and Adler JH (1991) Ecdysteroid distribution during development of spinach. Phytochemistry 30, 2905–2910.

    Article  CAS  Google Scholar 

  • Hahn DR and Lee MW (1991) Studies on the constituents of Achyranthes Radix (I) oleanolic acid bisdesmoside from the roots. Arch Pham Res 35, 456–460.

    Google Scholar 

  • Preston-Mafham J and Dinan L (2002) Phytoecdysteroid levels and distribution during development in Limnanthes alba Harthw. ex Benth. (Limnanthaceae). Z Naturforsch 57c, 144–152.

    Google Scholar 

  • Savchenko T, Whiting P, Vladimir Š, Underwood E, Sarker SD and Dinan L (1998) Distribution and identities of phytoecdysteroids in the genus Briza (Gramineae). Biochem Syst Ecol 26, 781–791.

    Article  CAS  Google Scholar 

  • Savchenko T, Blackford M, Sarker SD and Dinan L (2001) Phytoecdysteroids from Lamium spp: identification and distribution within plants. Biochem Syst Ecol 29, 891–900.

    Article  CAS  Google Scholar 

  • Soriano I, Riley I, Potter M and Bowers W (2004) Phytoecdysteroids: a novel defense against plant-parasitic nematodes. J Chem Ecol 30, 1885–1899.

    Article  CAS  Google Scholar 

  • Tomás J, Camps F, Claveria E, J. Coll, Melé E and Messeguer J (1992) Composition and location of phytoecdysteroids in Ajuga reptans in vivo and in vitro cultures. Phytochemistry 31, 1585–1591.

    Article  Google Scholar 

  • Zolotar R, Bykhovets A and Kovganko N (2001) Effect of certain phytoecdysteroids on larvae of colorado beetle Leptinotarsa decemlineata. Chem Nat Compd 37, 537–539.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Applied Life Science, Jeju National University, Jeju, 690-756, Republic of Korea

    Kyung Hwan Boo, Doseung Lee, Quyen Van Nguyen, Seong Beom Jin, Seungtae Kang, Cao Dang Viet, Se Pill Park, Dong-Sun Lee & Key Zung Riu

  2. Subtropical Horticulture Research Institute, Jeju National University, Jeju, 690-756, Republic of Korea

    Kyung Hwan Boo, Doseung Lee & Key Zung Riu

  3. Center for Environmental Research and Education, Hanoi National University of Education, Hanoi, Vietnam

    Quyen Van Nguyen

Authors
  1. Kyung Hwan Boo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Doseung Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Quyen Van Nguyen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Seong Beom Jin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Seungtae Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Cao Dang Viet
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Se Pill Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Dong-Sun Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Key Zung Riu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Dong-Sun Lee or Key Zung Riu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Boo, K.H., Lee, D., Van Nguyen, Q. et al. Fluctuation of 20-hydroxyecdysone in individual organs of Achyranthes japonica during reproductive growth stage and its accumulation into seed. J Korean Soc Appl Biol Chem 56, 335–338 (2013). https://doi.org/10.1007/s13765-013-3023-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13765-013-3023-y

Keywords

Search

Navigation