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Immobilization and biochemical characterization of choline oxidase onto bimetallic (Fe/Cu) MOF for sensitive determination of choline

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Abstract

Today, quantitative and qualitative diagnosis of choline is clinically essential. One way to deal with the limitations of traditional methods is using the bimetallic metal–organic framework (MOF)/enzyme-based biosensors. Unlike single metal, the bimetallic MOFs have important properties such as increasing MOF peroxidation like activity, increasing the stability, and maintenance of the enzyme in the MOF structure, etc. In this research, a biosensor system based on MOF‏(Fe–Cu)–ChOx is designed for colorimetric detection of choline. Our results showed that the optimum pH and temperature for the peroxidase-like activity of MOF-enzyme complex were 5.5 and 25–40‏ °C, respectively. Also, the linear area and the detection limit of this biosensor were 20–200 and 0.006 micromoles, respectively. The obtained recovery in the human serum choline assay was in the range of 95.15–103.1%, which, compared to other similar biosensors, shows the sensitivity, specificity, and reliability of the biosensor in biological samples. These results indicated that the synthesized peroxidase-mimicking MOF‏(Fe–Cu) could stabilize the choline oxidase and sense choline in the biological fluids.

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Acknowledgements

The authors express their gratitude to the Research Council of the Shahid Bahonar University of Kerman, Kerman (Iran), for financial support during the course of this project.

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The author(s) received no specific funding for this work.

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Authors and Affiliations

  1. Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran

    Niusha Pazhoh, Arastoo Badoei-dalfard & Zahra Karami

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  1. Niusha Pazhoh
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  2. Arastoo Badoei-dalfard
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  3. Zahra Karami
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Correspondence to Arastoo Badoei-dalfard.

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Pazhoh, N., Badoei-dalfard, A. & Karami, Z. Immobilization and biochemical characterization of choline oxidase onto bimetallic (Fe/Cu) MOF for sensitive determination of choline. J IRAN CHEM SOC 19, 2795–2809 (2022). https://doi.org/10.1007/s13738-022-02493-z

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  • DOI: https://doi.org/10.1007/s13738-022-02493-z

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