Abstract
This study evaluated the ameliorative effect of Amaranthus spinosus leaf methanol extract (ASLME) against doxorubicin-induced multi-organ damage in Sprague Dawley Rats. Forty-nine (49) male Sprague Dawley rats were randomly stratified into 7 groups with 7 rats per group. Groups A and B received distilled water for 7 days. Groups C, D, and E were pretreated for 7 days with 200 mg/kg silymarin, 500 and 1000 mg/kg ASLME, respectively followed by intraperitoneal injection of 20 mg/kg doxorubicin (DOX) to groups B, C, D, and E on the 8th day. Groups F and G were orally administered 500 and 1000 mg/kg ASLME respectively for 7 days with an intraperitoneal injection of distilled water on the 8th day. After 48 h of DOX administration, blood was withdrawn by cardiac puncture, and organs were excised for biochemical and histopathological assays. Pretreatment with ASLME decreased the levels of tissues malondialdehyde and nitric oxide as well as serum tumor necrosis factor alpha (TNF-α) with a concomitant (p < 0.05) increase in the levels of serum interleukin-10 (IL-10) and tissues reduced glutathione in a dose-dependent manner compared to group B. The activities of antioxidant enzymes increased significantly (p < 0.05) in the ASLME pretreated groups as well as groups F and G when compared to group B. Administration of doxorubicin induced degenerative hepatic, nephrotic and cardiac biomarkers and histological changes in Group B, while remarkable reversal of these pathological features was observed in groups pretreated with ASLME. Our findings suggest the chemo-protective effect of ASLME against doxorubicin-induced multi-organ damage, by alleviating oxidative stress and inflammation in rats.
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Abbreviations
- ASLME:
-
Amaranthus spinosus Leaf methanol extract.
- DOX:
-
Doxorubicin
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AOA-—Conceptualization, Methodology, Software, Validation, Formal analysis, investigation, resources, data curation, visualization, project administration, and writing; SLA—Validation, Data curation, visualization project administration, writing, reviewing, and editing; OOE—Formal analysis, investigation, data curation and project administration; Odubiyi AE- Validation and visualization; AAA—data curation and visualization; TMA—Formal analysis, investigation and project administration; SOA—Formal analysis, investigation, visualization, reviewing; BID—Data curation, visualization and project administration.
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The protocol for this study was approved by the National Health Research Ethics Committee of the College of Biosciences, Federal University of Agriculture, Abeokuta, Ogun State with Approval number: FUNAAB/HREC/08/20/305.
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Akinloye OA has no conflict of interest. Sulaimon LA has no conflict of interest. Ogunbiyi OE has no conflict of interest. Odubiyi AE has no conflict of interest. Adewale AA has no conflict of interest. Toriola MA has no conflict of interest. Salami OA has no conflict of interest. Boyenle ID has no conflict of interest.
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Akinloye, O.A., Sulaimon, L.A., Ogunbiyi, O.E. et al. Amaranthus spinosus (Spiny Pigweed) methanol leaf extract alleviates oxidative and inflammation induced by doxorubicin in male sprague dawley rats. ADV TRADIT MED (ADTM) 23, 1231–1248 (2023). https://doi.org/10.1007/s13596-022-00677-9
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DOI: https://doi.org/10.1007/s13596-022-00677-9