Abstract
The aim of this study is to investigate the protective effect of 70% methanolic extract of Myrtus communis L. leaves (300 mg/kg) against the kidney stone modeling created by (0.75%) ethylene glycole. Twenty-eight, 10 week’s Sprague Dawley male rats were divided into four groups (n = 7) as the control group, ethylene glycol (EG) group, M. communis (MC) group and protective (EG + MC) group. For 28 days, while 0.75% EG was given by adding it to drinking water to occur kidney stone model, MC was given by gavage to test the protective effect. 24 h’ urine samples were taken, and calcium, creatinine, uric acid, magnesium, citrate and oxalate were evaluated. On the 28th day, the rats were sacrificed, and their kidneys were removed. Kidney tissues were examined with hematoxylin eosin (HE) staining and kidney stones formed with Pizzolato’s (PZ) staining technique were shown. M. communis extract brought abnormal urine parameter values closer to the control group. Additionally, it was concluded that the tissue degeneration and number of stones decreased in the protective group. M. communis extract has a protective effect against the kidney stone modeling which created by EG.
This is a preview of subscription content, log in via an institution to check access.
taken from microscope lenses by HE staining (Ax20, Bx40). (H: hemorrhage, TD: Tubular dilatation, 
: Inflamation, black filled triangle: Vacuolization)
Similar content being viewed by others
References
Afkari R, Feizabadi MM, Ansari-Moghadam A et al (2019) Simultaneous use of oxalate-degrading bacteria and herbal extract to reduce the urinary oxalate in a rat model: a new strategy. Int Braz J Urol. https://doi.org/10.1590/S1677-5538.IBJU.2019.0167
Aleksic V, Knezevic P (2014) Antimicrobial and antioxidative activity of extracts and essential oils of Myrtus communis L. Microbiol Res 169(4):240–254
Alipour G, Dashti S, Hosseinzadeh H (2014) Review of pharmacological effects of Myrtus communis L. and its active constituents. Phyther Res 28
Amar A, Majmundar AJ, Ullah I et al (2019) Gene panel sequencing identifies a likely monogenic cause in 7% of 235 Pakistani families with nephrolithiasis. Hum Genet. https://doi.org/10.1007/s00439-019-01978-x
Atay E, Ertekin T, Yılmaz H et al (2019) Impact of prenatal exposure to bisphenol A on pregnant rats: fetal bone development and immunohistochemistry implications. Toxicol Ind Health. https://doi.org/10.1177/0748233718823146
Bawari S, Sah AN, Tewari D (2020) Anticalcifying effect of Daucus carota in experimental urolithiasis in Wistar rats. J Ayurveda Integr Med. https://doi.org/10.1016/j.jaim.2018.12.003
Bouanani S, Henchiri C, Migianu-Griffoni E et al (2010) Pharmacological and toxicological effects of Paronychia argentea in experimental calcium oxalate nephrolithiasis in rats. J Ethnopharmacol. https://doi.org/10.1016/j.jep.2010.01.056
Bouaoudia-Madi N, Boulekbache-Makhlouf L, Madani K et al (2019) Optimization of ultrasound-assisted extraction of polyphenols from myrtus communis L. Pericarp Antioxidants. https://doi.org/10.3390/antiox8070205
Chen Z, Bird VY, Ruchi R et al (2018) Development of a personalized diagnostic model for kidney stone disease tailored to acute care by integrating large clinical, demographics and laboratory data: the diagnostic acute care algorithm - kidney stones (DACA-KS). BMC Med Inform Decis Mak. https://doi.org/10.1186/s12911-018-0652-4
Ciccarelli D, Bottega S, Spanò C (2019) Study of functional and physiological response of co-occurring shrub species to the Mediterranean climate. Saudi J Biol Sci. https://doi.org/10.1016/j.sjbs.2018.08.021
Correddu F, Maldini M, Addis R et al (2019) Myrtus communis liquor byproduct as a source of bioactive compounds. Foods. https://doi.org/10.3390/foods8070237
Cruciani S, Garroni G, Ginesu GC et al (2020) Unravelling cellular mechanisms of stem cell senescence: an aid from natural bioactive molecules. Biology (basel). https://doi.org/10.3390/biology9030057
Cruciani S, Santaniello S, Fadda A et al (2019) Extracts from myrtle liqueur processing waste modulate stem cells pluripotency under stressing conditions. Biomed Res Int. https://doi.org/10.1155/2019/5641034
Dallas KB, Conti S, Liao JC et al (2017) Redefining the stone belt: precipitation is associated with increased risk of urinary stone disease. J Endourol. https://doi.org/10.1089/end.2017.0456
Dinnimath BM, Jalalpure SS, Patil UK (2017) Antiurolithiatic activity of natural constituents isolated from Aerva lanata. J Ayurveda Integr Med. https://doi.org/10.1016/j.jaim.2016.11.006
Dissayabutra T, Kalpongnukul N, Chindaphan K et al (2019) Urinary sulfated glycosaminoglycan insufficiency and chondroitin sulfate supplement in urolithiasis. PLoS ONE. https://doi.org/10.1371/journal.pone.0213180
Do Vale LMP, Ribeiro AM, Costa D et al (2020) Metabolic evaluation in urolithiasis – study of the prevalence of metabolic abnormalities in a tertiary centre. Cent Eur J Urol. https://doi.org/10.5173/ceju.2020.0051
Gambaro G, Croppi E, Coe F et al (2016) Metabolic diagnosis and medical prevention of calcium nephrolithiasis and its systemic manifestations: a consensus statement. J Nephrol. https://doi.org/10.1007/s40620-016-0329-y
Han DS, Yuk SM, Youn CS et al (2015) Primary mucinous cystadenocarcinoma of the renal pelvis misdiagnosed as ureteropelvic junction stenosis with renal pelvis stone: a case report and literature review. World J Surg Oncol. https://doi.org/10.1186/s12957-015-0739-7
Hennia A, Miguel M, Nemmiche S (2018) Antioxidant activity of Myrtus communis L. and Myrtus nivellei Batt. & Trab. extracts: a brief review. Medicines. https://doi.org/10.3390/medicines5030089
Jung SI, Kim YJ, Park HS et al (2010) Sensitivity of digital abdominal radiography for the detection of ureter stones by stone size and location. J Comput Assist Tomogr. https://doi.org/10.1097/RCT.0b013e3181ec7e07
Meng J, Xu Y, Li A et al (2018) Clinical features of 167 inpatients with autosomal dominant polycystic kidney disease at a single center in China. Med Sci Monit. https://doi.org/10.12659/MSM.910127
Mert T, Fafal T, Kivçak B, Öztürk HT (2008) Antimicrobial and cytotoxic activities of Myrtus communis L. Ankara Univ Eczac Fak Derg. https://doi.org/10.1501/eczfak_0000000501
Morel J, Mercuri AM, Mercuri AM, et al PaCE : a project for Europe of Pompeii
Ozbeyli D, Sen A, Cilingir Kaya OT et al (2020) Myrtus communis leaf extract protects against cerulein-induced acute pancreatitis in rats. J Food Biochem. https://doi.org/10.1111/jfbc.13130
Savaş Şen Z, Taşar MA, Demirel Kaya F et al (2019) Akut Cryptosporidium İshalinin Nadir Bir Komplikasyonu: İmmün Yetmezlik Bulunmayan Çocuk Olguda Olası Hepatobiliyer Tutulum. Mikrobiyol Bul. https://doi.org/10.5578/mb.68508
Shi J, Duan J, Gong H et al (2019) Exosomes from miR-20b-3p-overexpressing stromal cells ameliorate calcium oxalate deposition in rat kidney. J Cell Mol Med. https://doi.org/10.1111/jcmm.14555
Siracusa L, Napoli E, Tuttolomondo T et al (2019) A two-year bio-agronomic and chemotaxonomic evaluation of wild Sicilian Myrtle (Myrtus communis L.) berries and leaves. Chem Biodivers. https://doi.org/10.1002/cbdv.201800575
Sisay M, Gashaw T (2017) Ethnobotanical, ethnopharmacological, and phytochemical studies of Myrtus communis linn: a popular herb in unani system of medicine. J Evidence-Based Complement Altern Med 22
Sumbul S, Ahmad MA, Asif M et al (2010) Evaluation of Myrtus communis Linn. berries (common myrtle) in experimental ulcer models in rats. Hum Exp Toxicol. https://doi.org/10.1177/0960327110364154
Besufekad SY, Mekdes M, Abebech M, Delesa D, Tekalign D, Demitu K, Birtukan B (2017) The antimicrobial activity of leaf extracts of Myrtus communis. J Microb Biochem Tech 9(6):290–2
Wang X, Bhutani G, Vaughan LE et al (2020) Urinary monocyte chemoattractant protein 1 associated with calcium oxalate crystallization in patients with primary hyperoxaluria. BMC Nephrol. https://doi.org/10.1186/s12882-020-01783-z
Wells KA (2000) Nephrolithiasis with unusual initial symptoms. J Manipul Physiol Ther. https://doi.org/10.1016/S0161-4754(00)90249-5
Wróbel G, Kuder T (2019) The role of selected environmental factors and the type of work performed on the development of urolithiasis – a review paper. Int J Occup Med Environ Health 32(6):761–775
Yang A, Guo H, Fu M, Liu M (2019) Inhibitive effects of Huashi pill on formation of renal stones by modulating urine biochemical indexes and osteopontin in renal stone rat models. Med Sci Monit. https://doi.org/10.12659/MSM.916247
Yang X, Yang T, Li J et al (2019b) Metformin prevents nephrolithiasis formation by inhibiting the expression of OPN and MCP-1 in vitro and in vivo. Int J Mol Med. https://doi.org/10.3892/ijmm.2019.4084
Yilmaz S, Ülger H, Ertekin T et al (2019) Investigating the anti-tumoral effect of curcumin on the mice in which Ehrlich ascites and solid tumor is created. Iran J Basic Med Sci. https://doi.org/10.22038/ijbms.2019.33623.8019
Yılmaz H, Ertekin T, Atay E et al (2018) Antioxidant role of melatonin against nicotine’s teratogenic effects on embryonic bone development. Iran J Basic Med Sci. https://doi.org/10.22038/ijbms.2018.26705.6539
Funding
This study was produced from Halil Yılmaz’s PhD thesis “The effect of Myrtus Communis L. on Experimental Kidney Stone in Rats”. Financial source of this investigation was funded by Erciyes University Scientific Research Projects Coordination Unit (TDK-2018-8431).
Author information
Authors and Affiliations
Contributions
Conceptualization: H.Y. and N.E.; methodology: H.Y.. A.Ö. and H.Ü; validation: H.Y.. G. ¸S.K. and M.N.; formal analysis: H.Y.. A. H.Y. and G. Ş.K..; investigation: H.Y., S.Y. and Ş.A.; data curation: H.Y.. N.E. and M.T.; writing—original draft preparation: H.Y. and G.,S.K.; writing—review and editing: H.Y. and M.N. All authors have read and agreed to the published version of the manuscript.
Corresponding author
Ethics declarations
Ethical statement
The suitability of this study to the animal experiments procedure was approved by the Erciyes University Animal Experiments Local Ethics Committee decision dated 16/07/2018 and numbered 18/094 (Annex-1).
Conflict of interest
Halil Yılmaz has no conflict of interest. Nihat Ekinci has no conflict of interest. Ayşe Ömerli has no conflict of interest. Mehtap Nisari has no conflict of interest. Arzu Hanım Yay has no conflict of interest. Harun Ülger has no conflict of interest. Gökçe Şeker Karatoprak has no conflict of interest. Seher Yımaz has no conflict of interest. Şükrü Ateş has no conflict of interest. Mustafa Tastan has no conflict of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Yılmaz, H., Ekinci, N., Ömerli, A. et al. The protective effect of Myrtus communis L. against experimental kidney stone in rats. ADV TRADIT MED (ADTM) 23, 241–249 (2023). https://doi.org/10.1007/s13596-021-00620-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13596-021-00620-4