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Aqueous extract of purple Bordeaux radish, Raphanus sativus L. ameliorates ethanol-induced gastric injury in rats

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Abstract

The aim of this study was to evaluate whether the crude aqueous extract of purple Bordeaux radish (Raphanus sativus L.) (PBR) ameliorates ethanol-induced gastric injury in rats. Animals were divided into the following nine groups: normal, distilled water-treated vehicle control, groups orally administered PBR extract (250, 500 and 1,000 mg/kg, daily for 2 weeks), with or without ethanol treatment (5 ml/kg) 24 h after final administration of PBR extract, and a group treated with ethanol alone. Rats treated with PBR extract (250–1,000 mg/kg) for 2 weeks showed no significant effects on body weight and gastric mucosa. Pretreatment with PBR extract at doses of 500 and 1,000 mg/kg, but not 250 mg/kg, significantly ameliorated ethanol-induced gastric hemorrhages in rats (p < 0.05). The immunoreactivities of inducible nitric oxide (iNOS) and its byproduct nitrotyrosine in the gastric mucosa of ethanol-treated rats pretreated with 500 mg/kg PBR extract were significantly reduced, as compared with rats treated with ethanol alone (p < 0.05). Collectively, these findings suggest that pretreatment with PBR extract ameliorates ethanol-induced damage in the gastric mucosa, which is mediated partly by the anti-oxidative activity of the extract. The precise mechanism of this gastroprotective action of PBR extract should be investigated further.

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Acknowledgments

This research was supported by the Jeju Bio-industry Development Center, Jeju Technopark, Korea. The authors also would like to express their sincere thanks to undergraduate students in Laboratory of Veterinary Anatomy, College of Veterinary Medicine, Jeju National University for their technical assistance.

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Authors and Affiliations

  1. School of Medicine, Jeju National University, Jeju, 690-756, Republic of Korea

    Meejung Ahn

  2. Bio-industry Development Center, Jeju Technopark, Jeju, 690-121, Republic of Korea

    RyeoKyeong Koh & Gi Ok Kim

  3. College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju, 690-756, Republic of Korea

    Taekyun Shin

  4. Laboratory of Veterinary Anatomy, College of Veterinary Medicine, Jeju National University, Jeju, 690-756, Republic of Korea

    Taekyun Shin

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  1. Meejung Ahn
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  2. RyeoKyeong Koh
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  3. Gi Ok Kim
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  4. Taekyun Shin
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Correspondence to Gi Ok Kim or Taekyun Shin.

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Ahn, M., Koh, R., Kim, G.O. et al. Aqueous extract of purple Bordeaux radish, Raphanus sativus L. ameliorates ethanol-induced gastric injury in rats. Orient Pharm Exp Med 13, 247–252 (2013). https://doi.org/10.1007/s13596-013-0131-5

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  • DOI: https://doi.org/10.1007/s13596-013-0131-5

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