Abstract
Honey bees are important model organisms for neurobiology, because they display a large array of behaviors. To link behavior with individual gene function, quantitative polymerase chain reaction is frequently used. Comparing gene expression of different individuals requires data normalization using adequate reference genes. These should ideally be expressed stably throughout lifetime. Unfortunately, this is frequently not the case. We studied how well three commonly used reference genes are suited for this purpose and measured gene expression in the brains of honey bees differing in age and social role. Although rpl32 is used most frequently, it only remains stable in expression between newly emerged bees, nurse-aged bees, and pollen foragers but shows a peak at the age of 12 days. The genes gapdh and ef1α-f1, in contrast, are expressed stably in the brain throughout all age groups except newly emerged bees. According to stability software, gapdh was expressed most stably, followed by rpl32 and ef1α-f1.
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Acknowledgments
The authors thank Benedikt Polaczek for assistance in beekeeping. This work was supported by grants from the German Research Foundation (DFG) to RS (SCH1573/2-1, SCH1573/4-1) and WB (BL469/7-1).
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Manuscript editor: Stan Schneider
Possibilité d’utiliser trois gènes de référence communs pour une PCR quantitative en temps réel chez les abeilles
Expression génique / PCR quantitative / gène de référence / programme de stabilité / Apis mellifera
Untersuchung von drei häufig verwendeten Referenzgenen für quantitative Echtzeit-PCR in Honigbienen
Genexpression / quantitative PCR / Referenzgene / Stabilitätsprogramme / Apis mellifera
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Reim, T., Thamm, M., Rolke, D. et al. Suitability of three common reference genes for quantitative real-time PCR in honey bees. Apidologie 44, 342–350 (2013). https://doi.org/10.1007/s13592-012-0184-3
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DOI: https://doi.org/10.1007/s13592-012-0184-3