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Chromosomal analysis of Lilium longiflorum x Asiatic hybrids using GISH (genomic in situ hybridization)

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Abstract

In Lilium, a popular horticulture crop, the main objective of crossbreeding is introgression of desirable genes and features into new cultivars. Commercial Lilium cultivars were produced primarily through 2n-gametes or 2x-gametes as parental plants or backcrossing. The primary genetic difference in 2 methods, is the presence of genomic recombination. Especially, GISH (genomic in situ hybridization) among molecular cytogenetic analysis, is the relevant technique to detect genetic information; genome composition, chromosome behavior during meiosis and recombination of hybrids as using their parental DNA as a probe. Based on previous studies, in this research, 12 LA (L. longiflorum x L. Asiatic hybrids) hybrids were analyzed by GISH (genomic in situ hybridization) along with ploidy analysis, and pollen germination tests. The LA hybrids used in this study, showed less pollen germination ability (ranged 0 - 21.74%), but germination of ‘Caesars Palace’ was significantly higher (59.09%). Interestingly, ploidy analysis showed that ‘Caesars Palace’ was tetraploid and ‘Batistero’ had one more additional chromosome from the L genome. Nine of the 12 cultivars exhibited recombination and 3 had only non-recombinant chromosomes; fewer L. longiflorum chromosomes were present in the cultivars than Asiatic chromosomes. Consequentially, it is assumed that 2n-gametes were more common methodology than 2x-gametes for producing commercial cultivars. Moreover, backcrossing tended to be performed with the Asiatic hybrid cultivar.

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Authors and Affiliations

  1. Department of Horticulture, Kyungpook National University, Daegu, 41566, Republic of Korea

    Min-Ji Kwon, Fahad Ramzan, Yun-Jae Ahn, Chang-Kil Kim & Ki-Byung Lim

  2. Institute of Agricultural Science and Technology, Kyungpook National University, Daegu, 41566, Republic of Korea

    Ki-Byung Lim

  3. Department of Life Science, Sahmyook University, Seoul, 01795, Republic of Korea

    Yoon-Jung Hwang

  4. Floriculture Research Division, National Institute of Horticultural & Herbal Science, Wanju-gun, 55465, Republic of Korea

    Yun-Im Kang

  5. Institute of Horticultural Sciences, University of Agriculture, Faisalabad, 38040, Pakistan

    Adnan Younis

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  1. Min-Ji Kwon
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  2. Fahad Ramzan
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  3. Yun-Jae Ahn
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  8. Ki-Byung Lim
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Correspondence to Ki-Byung Lim.

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Kwon, MJ., Ramzan, F., Ahn, YJ. et al. Chromosomal analysis of Lilium longiflorum x Asiatic hybrids using GISH (genomic in situ hybridization). Hortic. Environ. Biotechnol. 58, 591–600 (2017). https://doi.org/10.1007/s13580-017-0019-2

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  • DOI: https://doi.org/10.1007/s13580-017-0019-2

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