Skip to main content
SpringerLink
Log in

Genetic variations of Chinese melon landraces investigated with EST-SSR markers

  • Research Report
  • Published:
Horticulture, Environment, and Biotechnology Aims and scope Submit manuscript

Abstract

SSR markers derived from expressed sequence tags (EST-SSR) are valuable tools for genetic variation assessment because they potentially exhibit functional diversity. The genetic variations of 27 melon accessions, including 21 thin-skinned melon landraces which nearly represent all types of thin-skinned melon landraces originated in China, were analyzed with EST-SSR markers. Of the 22 EST-SSR markers employed, 19 EST-SSR detected polymorphism with 13 of which had putative functions. The number of alleles detected by EST-SSR ranged from 2 to 5 with the average of 3. The PIC value for each locus varied from 0.21 to 0.68 with the mean of 0.46. Cluster analysis by UPGMA partitioned the accessions into groups of thin-skinned melon and thick-skinned melon with high bootstrap value. In the thin-skinned group, the EST-SSR markers failed to discriminate the thin-skinned vegetable melon from the other thin-skinned melon accessions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  • Andersen, J.R. and T. Lubberstedt. 2003. Functional markers in plants. Trends Plant Sci. 8:554–560.

    Article  PubMed  CAS  Google Scholar 

  • Anderson, J.A., G.A. Churchill, J.E. Autrique, S.D. Tanksley, and M.E. Sorrells. 1993. Optimizing parental selection for genetic linkage maps. Genome 36:181–186.

    Article  PubMed  CAS  Google Scholar 

  • Chabane, K., G.A. Ablett, G.M. Cordeiro, J. Valkoun, and R.J. Henry. 2005. EST versus genomic derived microsatellite markers for genotyping wild and cultivated barley. Genet. Resour. Crop Evol. 52:903–909.

    Article  CAS  Google Scholar 

  • Chabane, K., R. Varshney, A. Graner, and J. Valkoun. 2008. Generationand exploitation of EST-derived SSR markers for assaying molecular diversity in durum wheat populations. Genet. Resour. Crop Evol. 55:869–881.

    Article  CAS  Google Scholar 

  • Chiba, N., K. Suwabe, T. Nunome, and M. Hirai. 2003. Development of microsatellite markers in melon (Cucumis melo L.) and their application to major cucurbit crops. Breeding Sci. 53:21–27.

    Article  CAS  Google Scholar 

  • Cordeiro, G.M, R. Casu, C.L. McIntyre, J.M. Manners, and R.J. Henry. 2001. Microsatellite markers from sugarcane (Saccharum spp.) ESTs cross transferable to erianthus and sorghum. Plant Sci. 160:1115–1123.

    Article  PubMed  CAS  Google Scholar 

  • Danin-Poleg, Y., N. Reis, G. Tzuri, and N. Katzir. 2001. Development and characterization of microsatellite markers in Cucumis. Theor. Appl. Genet. 102:61–72.

    Article  CAS  Google Scholar 

  • Fukino, N., Y. Sakata, M. Kunihisa, and S. Matsumoto. 2007. Characterisation of novel simple sequence repeat (SSR) markers for melon (Cucumis melo L.) and their use for genotype identification. J. Hortic. Sci. Biotechnol. 82:330–334.

    CAS  Google Scholar 

  • Gonzalo, M.J., M. Oliver, J. Garcia-Mas, A.J. Monforte, R. Dolcet-Sanjuan, N. Katzir, P. Arus, and A. Monforte. 2005. Simple-sequence repeat markers used in merging linkage maps of melon (Cucumis melo L.). Theor. Appl. Genet. 110:802–811.

    Article  PubMed  CAS  Google Scholar 

  • Guo, W.Z., C.P. Cai, C.B. Wang, Z.G. Han, X.L. Song, K. Wang, X.W. Niu, C. Wang, K.Y. Lu, B. Shi, and T.Z. Zhang. 2007. A microsatellite-based, gene-rich linkage map reveals genome structure, function and evolution in gossypium. Genetics 176:527–541.

    Article  PubMed  CAS  Google Scholar 

  • Gupta, P.K., S. Rustgi, S. Sharma, R. Singh, N. Kumar, and H.S. Balyan. 2003. Transferable EST-SSR markers for the study of polymorphism and genetic diversity in bread wheat. Mol. Genet. Genomics 270:315–323.

    Article  PubMed  CAS  Google Scholar 

  • Katzir, N., Y. Danin-Poleg, G. Tzuri, Z. Karchi, U. Lavi, and P.B. Cregan. 1996. Length polymorphism and homologies of microsatellites in several Cucurbitaceae species. Theor. Appl. Genet. 93:1282–1290.

    Article  CAS  Google Scholar 

  • Kong, Q., C. Xiang, Z. Yu, C. Zhang, F. Liu, C. Peng, and X. Peng. 2007. Mining and charactering microsatellites in Cucumis melo expressed sequence tags from sequence database. Mol. Ecol. Notes 7:281–283.

    Article  CAS  Google Scholar 

  • Laurent, V., P. Devaux, T. Thiel, F. Viard, S. Mielordt, P. Touzet, and M. Quillet. 2007. Comparative effectiveness of sugar beet microsatellie markers isolated from genomic libraries and GenBank ESTs to map the sugar beet genome. Theor. Appl. Genet. 115:793–805.

    Article  PubMed  CAS  Google Scholar 

  • Luan, F., I. Delannay, and J. Staub. 2008. Chinese melon (Cucumis melo L.) diversity analyses provide strategies for germplasm curation, genetic improvement, and evidentiary support of domestication patterns. Euphytica 164:445–461.

    Article  CAS  Google Scholar 

  • Metzgar, D., J. Bytof, and C. Wills. 2000. Selection against frameshift mutations limits microsatellite expansion in coding DNA. Genome Res. 10:72–80.

    PubMed  CAS  Google Scholar 

  • Monforte, A.J., J. Garcia-Mas, P. Arus, and E. Minch. 2003. Genetic variability in melon based on microsatellite variation. Plant Breeding 122:153–157.

    Article  Google Scholar 

  • Nakata, E., J. Staub, A. Lopez-Sese, and N. Katzir. 2005. Genetic diversity of Japanese melon cultivars (Cucumis melo L.) as assessed by random amplified polymorphic DNA and simple sequence repeat markers. Genet. Resour. Crop Evol. 52:405–419.

    Article  CAS  Google Scholar 

  • Powell, W., G.C. Machray, and J. Provan. 1996. Polymorphism revealed by simple sequence repeats. Trends Plant Sci. 1:215–222.

    Google Scholar 

  • Ritschel, P.S., T.C. Lins, R.L. Tristan, G.S. Buso, J.A. Buso, and M.E. Ferreira. 2004. Development of microsatellite markers from an enriched genomic library for genetic analysis of melon (Cucumis melo L.). BMC Plant Biol. 4:9.

    Article  PubMed  Google Scholar 

  • Robinson, R.W. and D.S. Decker-Walters. 1997. Cucurbits. CAB International, New York, USA.

    Google Scholar 

  • Sensoy, S., S. Buyukalaca, and K. Abak. 2007. Evaluation of genetic diversity in Turkish melons (Cucumis melo L.) based on phenotypic characters and RAPD markers. Genet. Resour. Crop Evol. 54:1351–1365.

    Article  Google Scholar 

  • Staub, J.E., J. Bacher, and K. Poetter. 1996. Factors affecting the application of random amplified polymorphic DNAs in cucumber (Cucumis sativus L.). Hortic. Sci. 31:262–266.

    CAS  Google Scholar 

  • Szabó, Z., G. Gyulai, M. Humphreys, L. Horváth, A. Bittsánszky, R. Lágler, and L. Heszky. 2005. Genetic variation of melon (C. melo) compared to an extinct landrace from the Middle Ages (Hungary) I. rDNA, SSR and SNP analysis of 47 cultivars. Euphytica 146:87–94.

    Article  Google Scholar 

  • Varshney, R.K., A. Graner, and M.E. Sorrells. 2005. Genic microsatellite markers in plants: features and applications. Trends in Biotechnol. 23:48–55.

    Article  CAS  Google Scholar 

  • Yashiro, K., H. Iwata, Y. Akashi, K.O. Tomita, M. Kuzuya, Y. Tsumura, and K. Kato. 2005. Genetic relationship among East and South Asian melon (Cucumis melo L.) revealed by AFLP analysis. Breeding Sci. 55:197–206.

    Article  CAS  Google Scholar 

  • Yi, G.B., J.M. Lee, S. Lee, D. Choi, and B.D. Kim. 2006. Exploitation of pepper EST-SSRs and an SSR-based linkage map. Theor. Appl. Genet. 114:113–130.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Center for Vegetable Improvement; Key Laboratory of Horticultural Plant Biology, Ministry of Education; College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan, 430070, China

    Qiusheng Kong, Changping Xiang, Jin Yang & Zhongwei Yu

Authors
  1. Qiusheng Kong
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Changping Xiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jin Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhongwei Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Qiusheng Kong.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kong, Q., Xiang, C., Yang, J. et al. Genetic variations of Chinese melon landraces investigated with EST-SSR markers. Hortic. Environ. Biotechnol. 52, 163–169 (2011). https://doi.org/10.1007/s13580-011-0087-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13580-011-0087-7

Additional key words

Search

Navigation