Abstract
MCM3AP-AS1 regulates the cartilage repair in osteoarthritis, but how it regulates osteogenic differentiation of dental pulp stem cells (DPSCs) remains to be determined. DPSCs were isolated and induced for osteogenic differentiation. MCM3AP-AS1 expression was increased along with the osteogenic differentiation of DPSCs, whose expression was positive correlated with those of OCN, alkaline phosphatase (ALP) and RUNX2. On contrary, miR-143-3p expression was decreased along with the osteogenic differentiation and was negatively correlated with those of OCN, ALP and RUNX2. Dual-luciferase reporter gene assay showed that miR-143-3p can be negatively regulated by MCM3AP-AS1 and can regulate IGFBP5. MCM3AP-AS1 overexpression increased the expression levels of osteogenesis-specific genes, ALP activity and mineralized nodules during DPSC osteogenic differentiation, while IGFBP5 knockdown or miR-143-3p overexpression counteracted the effect of MCM3AP-AS1 overexpression in DPSCs. Therefore, this study demonstrated the role of MCM3AP-AS1/miR-143-3p/IGFBP5 axis in regulating DPSC osteogenic differentiation.
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Thanks for the grants from the Xiamen Science and Technology Planned Project of Medical Health (No. 3502Z20194079), Medical Health Research project of Youth in Fujian Province (2019-2-56), Medical Health Research project in Fujian Province (2019-2-55) and Fujian Province Educational Science “13th Five-Year Plan” 2020 Project (No. FJJKCG20-015).
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CY is the guarantor of integrity of the entire study and contributed to the experimental studies and manuscript preparation. LY: contributed to the experimental studies. XX: contributed to the manuscript preparation and statistical analysis. PL: contributed to the experimental studies. BL: contributed to the experimental studies and manuscript preparation. SL: contributed to the analysis and interpretation of data. HH: contributed to the literature research. JZ: contributed to the data acquisition and literature research. MH: contributed to the statistical analysis. SP and QW: drafted the article and revised it critically. All the authors read and approved the final manuscript.
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The extraction of mandibular third molars was performed with the permission of the Ethics Committee of Stomatological Hospital of Xiamen Medical College & Xiamen Key Laboratory of Stomatological Disease Diagnosis and Treatment, and the study is provided with informed consent of all participants.
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Yang, C., Xu, X., Lin, P. et al. Overexpression of long noncoding RNA MCM3AP-AS1 promotes osteogenic differentiation of dental pulp stem cells via miR-143-3p/IGFBP5 axis. Human Cell 35, 150–162 (2022). https://doi.org/10.1007/s13577-021-00648-3
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DOI: https://doi.org/10.1007/s13577-021-00648-3