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RING-finger protein 5 promotes hepatocellular carcinoma progression and predicts poor prognosis

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Abstract

Hepatocellular carcinoma (HCC) is one of the most common cause of malignancy-related deaths. Recently, RING-finger protein 5 (RNF5), an E3 ubiquitin ligase, was revealed to be associated with the development of several human cancers. However, the clinical implication and functional role of RNF5 in HCC are poorly understood. We analysed RNF5 expression in HCC samples and observed that both the mRNA and protein levels of RNF5 were significantly increased in HCC tissues. RNF5 upregulation was markedly associated with larger tumour size, more satellite foci, and higher alpha fetoprotein (AFP) level, indicating poor prognosis in patients with HCC. Knockdown and overexpression experiments demonstrated that RNF5 promoted the proliferation, migration, and invasion of HCC cells in vitro. Moreover, RNF5 facilitated HCC growth in vivo. Our findings indicated that RNF5 was an oncogene of HCC progression and could be used as a novel prognostic biomarker and therapeutic target for patients with HCC.

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Funding

This study was supported by the National Natural Science Foundation of China (Grant numbers: 81871963, 81871961 and 81572335) and Medical and Health Key project of Xiamen (3502Z20191106).

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Authors and Affiliations

  1. School of Clinical Medicine, Fujian Medical University, Fuzhou, China

    Yi Zhang, Changmao Zhang, Song You & Xiaomin Wang

  2. Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma, Department of Hepatobiliary Surgery, Zhongshan Hospital of Xiamen University, 209 South Hubin Road, Xiamen, 361004, Fujian Province, China

    Jie Li, Yue Zhao, Yaqi Yu, Fei Fang, Ting Fang & Xiaomin Wang

  3. Department of Anus and Intestine Surgery, Beijing University of Chinese Medicine Affiliated Xiamen Hospital, Xiamen, China

    Huayang Chen

  4. Department of Neurosurgery, Zhongshan Hospital of Xiamen University, Xiamen, China

    Xiaoning Lin

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  1. Yi Zhang
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  2. Jie Li
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  3. Huayang Chen
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  4. Changmao Zhang
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  5. Song You
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  7. Xiaoning Lin
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  8. Yaqi Yu
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  9. Fei Fang
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  10. Ting Fang
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  11. Xiaomin Wang
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Corresponding author

Correspondence to Xiaomin Wang.

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The authors declared that they have no conflicts of interest to this work.

Ethical standards

The use of specimens was approved by the institutional research ethics committee of Zhongshan Hospital of Xiamen University (number: 2015–02-08). All animal experiments were approved by the ethical committee of Xiamen University and under the Guide for the Care and Use of Laboratory Animals by NIH.

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Zhang, Y., Li, J., Chen, H. et al. RING-finger protein 5 promotes hepatocellular carcinoma progression and predicts poor prognosis. Human Cell 34, 530–538 (2021). https://doi.org/10.1007/s13577-020-00460-5

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  • DOI: https://doi.org/10.1007/s13577-020-00460-5

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